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Application of astragaloside IV in preparation of drugs for treating and delaying intervertebral disc degeneration

A technology of astragaloside IV and degeneration, which is applied in the direction of drug combination, pharmaceutical formula, organic active ingredients, etc., can solve the problem of whether the intervertebral disc degenerative disease has a therapeutic effect, etc., and achieve obvious effect and moderate anti-apoptotic effect , the effect of low dosage

Inactive Publication Date: 2018-06-22
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Astragaloside IV has shown good biological activities in a number of pharmacological studies, such as enhancing the body's immunity, improving the body's disease resistance, anti-virus effect, anti-stress effect, as a growth-promoting agent, improving cardiopulmonary function, etc. But whether it has a therapeutic effect on degenerative disc disease is unclear

Method used

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  • Application of astragaloside IV in preparation of drugs for treating and delaying intervertebral disc degeneration
  • Application of astragaloside IV in preparation of drugs for treating and delaying intervertebral disc degeneration
  • Application of astragaloside IV in preparation of drugs for treating and delaying intervertebral disc degeneration

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Experimental program
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Embodiment 1

[0024] 1. Experimental steps:

[0025] (1) Nucleus pulposus cell culture

[0026] Fifty adult SD rats were sacrificed with an overdose of sodium pentobarbital, and the lumbar discs (L1-L6) were carefully collected under aseptic conditions. Isolate the nucleus pulposus tissue from the lumbar intervertebral disc with a dissecting microscope, and treat the tissue with 0.1% collagenase and 2 U / ml hyaluronidase at 37 °C for 4 h, and then transfer the digested tissue as an explant at 37 °C containing 10% Fetal bovine serum (FBS; Hyclone) in Dulbecco's modified Eagle's medium (DMEM; Gibco). When confluent, cells were harvested by 0.25% trypsin-EDTA (Gibco), then counted and re-seeded at the appropriate density in 10 cm dishes. During the passaging process, no significant changes in cell morphology were observed between primary cells (passage 0) and subsequent passages (passage 2), so we used secondary cells cultured in monolayers for all experiments.

[0027] (2) Drug treatment

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Abstract

The invention relates to application of astragaloside IV in the preparation of drugs for treating and delaying intervertebral disc degeneration. Astragaloside IV serving as a plant extract with good biological activity has the advantages that the drug dosage is low and the anti-apoptotic effect on nucleus pulposus cells is obvious; compared with Western medicine, astragaloside IV serving as traditional Chinese medicine is mild in anti-apoptotic effect, and an alternative treatment drug can be provided for the comprehensive treatment of intervertebral disc degeneration diseases.

Description

technical field [0001] The invention relates to the application of astragaloside IV, in particular to the application of astragaloside IV in the preparation of medicines for treating and delaying intervertebral disc degeneration. Background technique [0002] Low back pain is a common disease in orthopedics, and it is more likely to occur in people aged 40 to 80. Severe low back pain can make patients incapable of work and impose a heavy burden on society and families. More than 40% of low back pain is caused by disc degeneration. But so far, there is no clinically available drug for the treatment of intervertebral disc degeneration. [0003] Intervertebral disc degeneration can be caused by a variety of factors, such as abnormal stress, local nutritional deficiency, and congenital genetic abnormalities, among which oxidative stress is one of the main pathogenic mechanisms of intervertebral disc degeneration, and excessive oxidative stress can cause nucleus pulposus cell a...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P19/08
CPCA61K31/7048
Inventor 田乃锋王晓斌陈徳横张小磊徐华梓
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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