NIRS (near infra red spectrum) model construction method for predicting saccharose content in sweet corn

Abstract

The invention discloses an NIRS (near infra red spectrum) model construction method for predicting saccharose content in sweet corn. The method comprises the following steps: (1) collection of near infrared spectra of a sample: obtaining near infrared spectrum information of the sample; (2) preparation of an enzyme method sample solution; (3) determination of sample saccharose: obtaining a chemical value of the sample; and (4) NIRS modeling: carrying out scatter correction and mathematical treatment of the spectrum on a saccharose calibration model. Specific to the saccharose content in the sweet corn, the chemical value determined by an infrared spectrum analysis combined with enzyme method is adopted, by the scatter correction and mathematical treatment of the spectrum, the analytic statistics of chemometrics are carried out on a full-spectrum wave band, an NIRS model of the saccharose content is constructed, the NIRS model can be used for quickly predicting the saccharose content inthe sweet corn, and then valuable references are provided for accelerating superior seed quality resource screening and quality breeding of the sweet corn.

Description

technical field [0001] The invention relates to the field of plant breeding, in particular to the detection of sucrose content in sweet corn. Background technique [0002] Near-infrared reflectance spectroscopy (NIRS) technology radiates the sample through near-infrared beams, and collects the reflectance or transmittance of the beam response. The basic principle is that the measured organic matter contains hydrogen-bearing groups (X-H) or carbon-carbon Chemical bonds such as double bonds (C-C), these chemical bonds will undergo overtone vibration or rotation under the irradiation of near-infrared beams, and then obtain the absorption spectrum in the near-infrared region by diffuse reflection. The chemical composition of different substances in the sample contains different hydrogen-containing groups, such as starch, sucrose, glucose, fructose and water-soluble polysaccharides, etc. all have different hydrogen-containing groups. These different hydrogen-containing groups ca...

AI Technical Summary

Problems solved by technology

At present, the methods for measuring the sugar content of sweet corn mainly adopt chemical detection methods such as high performance liquid chromatography, sugar meter method, anthrone colorimetric method, 3,5-dinitrosalicylic acid method, enzymatic hydrolysis method, etc., but these The method has the disadvantages of high cost, polluting the environment, and requiring high operating techniques.

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