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Multidimensional counter current chromatograph for circulating separating of complex sample and working method

A countercurrent chromatograph and complex sample technology, applied in the field of separation equipment, can solve the problems of a large number of instruments, poor separation effect, poor separation effect of multiple complex samples, etc., and achieve the effect of good separation effect and reduction of the number of instruments

Pending Publication Date: 2018-06-29
SHANDONG ANALYSIS & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] 1. The number of instruments used is large, and it is impossible to switch between one-dimensional separation and two-dimensional separation
[0005] 2. For samples that cannot be separated by two-dimensional countercurrent chromatography, the cyclic separation cannot be performed, and the separation effect is poor
[0006] 3. The separation effect of multiple complex samples is poor

Method used

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  • Multidimensional counter current chromatograph for circulating separating of complex sample and working method
  • Multidimensional counter current chromatograph for circulating separating of complex sample and working method
  • Multidimensional counter current chromatograph for circulating separating of complex sample and working method

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Embodiment Construction

[0048] It should be pointed out that the following detailed description is exemplary and intended to provide further explanation to the present application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

[0049]It should be noted that the terminology used here is only for describing specific implementations, and is not intended to limit the exemplary implementations according to the present application. As used herein, unless the context clearly dictates otherwise, the singular is intended to include the plural, and it should also be understood that when the terms "comprising" and / or "comprising" are used in this specification, they mean There are features, steps, operations, means, components and / or combinations thereof.

[0050] As introduced in the background technology, the existing two-dimensional countercurrent chromatography use...

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Abstract

The invention relates to a multidimensional counter current chromatograph for circulating separating of a complex sample and a working method. The multidimensional counter current chromatograph comprises a one-dimensional separating channel, a first three-way valve, a plurality of six-way valves, a two-dimensional separating channel and a circulating valve component which are connected in sequence; the circulating valve component forms a circulating separating channel; collecting pipes are arranged on the plurality of the six-way valves, and the collecting pipes of the different six-way valvesare connected into the circulating separating channel, so that different circulating peaks can be received; a plurality of complex samples can be separated and collected. According to the two-dimensional counter current chromatograph provided by the invention, rapid switching among one-dimensional separating, two-dimensional separating and circulating separating can be realized; a multi-componentcomplex sample can be separated; few instruments are used; a separating effect is good.

Description

technical field [0001] The invention relates to the technical field of separation equipment, in particular to a multi-dimensional countercurrent chromatograph and a working method for circular separation of complex samples. Background technique [0002] High-speed countercurrent chromatography is a liquid-liquid chromatography separation technology. Its stationary phase and mobile phase are both liquid without irreversible adsorption. It has the advantages of no loss of samples, no pollution, high efficiency, rapidity and large preparation. Two-dimensional countercurrent Chromatography is a new chromatographic analysis technology developed on the basis of traditional one-dimensional countercurrent chromatography. Its main principle is to connect two chromatographic columns with different separation mechanisms in series in a series manner. The separated components are further separated in the second dimension, achieving the effect of orthogonal separation. [0003] Conventio...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/42
CPCG01N30/06G01N30/42G01N2030/065
Inventor 王岱杰闫慧娇宋祥云付瑞明李丽丽
Owner SHANDONG ANALYSIS & TEST CENT