Kit for determining content of thyroglobulin antibodies and testing method of kit

Abstract

The invention discloses a kit for determining the content of thyroglobulin antibodies (TGAb) in human serum by virtue of a magnetic particle chemiluminiscence method. The kit comprises a calibration product, a quality control product, a resistance reagent, a magnetic particle reagent and a luminescent substrate, wherein the resistance reagent contains a fluorescein isothiocyanate labeled thyroglobulin coating antigen and an alkaline phosphatase labeled thyroglobulin coating antigen; and the magnetic particle reagent is a conjugate of a magnetic particle and a goat-anti FITC. According to the kit, a chemiluminiscence technique is combined with immune magnetic particles, an approximately homogeneous reaction system is provided, and a one-step method reaction mode is adopted, so that the detection sensitivity and the precision are greatly improved, the detection range is expanded, the reaction time is greatly shortened, the time period from the starting of sample feeding to the acquiringof a detection result is less than 35 minutes and is obviously shorter than similar kits; and multiple samples can be simultaneously determinate on a full-automatic chemiluminescence device, so that the high-flux rapid determination of the thyroglobulin antibodies is realized, the accuracy is high, the specificity is strong, and the precision and the detection efficiency are greatly improved.

Description

technical field [0001] The invention relates to the technical field of biochemistry, in particular to a kit for measuring the content of thyroglobulin antibody (TGAb) in a human body by using a magnetic particle chemiluminescence method. Background technique [0002] Thyroglobulin (TG) is a glycoprotein in thyroid follicular columnar cells with a molecular weight of 660KD and plays an important role in the synthesis and storage of thyroid hormones. In the body, thyroglobulin contains T3 and T4 in the form of colloid, which is stored in the follicle cavity. Under the action of enzymes, T3 and T4 are released into the cytoplasm and enter the blood circulation. At the same time, a small amount of TG is also released into the blood, and normal people can also detect TG with a concentration of 3-40ug / L. However, when the thyroid gland is damaged due to pathological reasons, a large amount of TG is released into the blood, and TG acts as an autoantigen to induce the production of...

AI Technical Summary

Problems solved by technology

The steps of radioimmunoassay are cumbersome, the reagents are expensive, and supporting equipment is required and there is radioactive contamination

Enzyme-linked immunosorbent assay has long detection time, complicated operation, poor repeatability, and is not suitable for emergency and clinical patients in time for diagnosis.

Latex-enhanced immunoturbidimetric method is simple and fast to operate, but has low sensitivity and poor reproducibility at low values

Images