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Functional markers related to drought resistance in budding stage of rapeseed and its application

A technology of drought resistance and rapeseed, applied in the fields of rapeseed breeding and molecular biology, can solve the problems of long cycle, heavy workload of drought resistance identification, easy to be affected by the environment, etc., and achieve the effect of improving selection efficiency and speeding up the pace of genetic improvement.

Active Publication Date: 2020-09-25
HUBEI ENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is how to efficiently and accurately identify the drought resistance of rape at the budding stage, so as to assist the breeding of rapeseed, and then solve the problems of large workload, long period and easy environmental influence in the process of drought resistance identification in the breeding process

Method used

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  • Functional markers related to drought resistance in budding stage of rapeseed and its application
  • Functional markers related to drought resistance in budding stage of rapeseed and its application
  • Functional markers related to drought resistance in budding stage of rapeseed and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Obtainment of the functional marker BnDM-413 related to the drought resistance of rape bud stage

[0054] 1. Group construction:

[0055] The strong drought-resistant rapeseed variety Qinyou No. 8 (leaf wilting index of 0.06, known and commonly used variety) was used as the female parent, and the drought-sensitive rapeseed variety Huyou 16 (leaf wilting index of 0.94, known and commonly used variety) was used as the male parent. A drought-resistant segregating F 2:3 There are 183 strains in total in the generation family group.

[0056] 2. DNA extraction

[0057] (1) Collection of parents and F 2 From the young leaves of 183 individual plants in the population, the total DNA of the leaves was extracted by the CTAB method. The specific extraction steps are as follows:

[0058] (1) Put 0.5 g of leaves fresh or frozen at -20°C into a 1.5ml centrifuge tube, grind to a homogenate with a glass rod, add 800 μl of CTAB extract (50mmol / L Tris-HCl, pH8.0; 20mmol / L ...

Embodiment 2

[0089] Example 2 Application of Functional Marker BnDM-413 in Identifying Drought Resistance of Rapeseed Budding Stage

[0090] 1. Group construction:

[0091] The strong drought-resistant rapeseed variety Qinyou No. 8 (leaf wilting index of 0.06, known and commonly used variety) was used as the female parent, and the drought-sensitive rapeseed variety Huyou 16 (leaf wilting index of 0.94, known and commonly used variety) was used as the male parent. F 1 generation group, F 1 F 2 generation groups.

[0092] 2. DNA extraction

[0093] from F 2 100 individual plants were randomly selected from the generation population, and their DNA was extracted. The specific operation steps were the same as the DNA extraction method in Example 1.

[0094] 3. PCR amplification

[0095] Using the above-mentioned extracted DNA as a template, the primer BnDM-413-F and primer BnDM-413-R of the functional marker BnDM-413 were used for PCR amplification,

[0096] Primer BnDM-413-F is: 5'-GCC...

Embodiment 3

[0112] Example 3 Application of Functional Marker BnDM-413 in Identifying Drought Resistance of Rapeseed Budding Stage

[0113] With reference to the method of Example 2, the scope of identification was expanded. A total of analyzes detected F 2 183 individual plants of the population, the results showed that F 2 There are 76 individual plants with bands in the population, and 76 F 2:3 The average wilting index of the generation family group was 0.305, which was significantly lower than the group's average value of 0.567 (P<0.001), that is, the drought resistance was significantly improved. The above results fully demonstrate that the marker can indeed be used for prediction, identification and screening of drought resistance.

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Abstract

The invention provides a functional marker related to the drought resistance of rape in the squaring to initial flowering stage, and an application thereof, and relates to the technical fields of rapebreeding and molecular biology. Rape varieties with different drought resistances are used as parents, and are hybridized to construct an F2 generation population and an F2:3 generation family population; and the SRAP molecular marker data of the leaves of the F2 generation population and the drought resistance phenotype data of the F2:3 generation family population are associatively analyzed, the most significantly associated molecular marker is obtained through screening, and is converted into a functional marker for drought resistance-assisted breeding, and the functional marker for drought resistance-assisted breeding is named as the functional marker BnDM-413. The invention also provides the application of the functional marker BnDM-413 in the identification of the drought resistanceand breeding of rape. The functional marker BnDM-413 is screened to efficiently, quickly and accurately identify the drought resistance of the rape and significantly improve the selection efficiencyof the drought resistance.

Description

technical field [0001] The invention relates to the technical fields of rapeseed breeding and molecular biology, in particular to a functional marker related to drought resistance in budding stage of rapeseed and its application. Background technique [0002] Crops are often subjected to various biotic and abiotic stresses during their growth, among which drought is one of the most serious abiotic stresses affecting crop growth and yield. Rapeseed is an important oil crop in the world. my country's annual rapeseed planting area and total output account for about 1 / 3 of the world's total. Drought directly affects the growth of rapeseed, leading to a significant decline in the total number of pods, the number of seeds per pod, the number of seeds per plant and the yield per plant, seriously affecting the yield of rapeseed. The Yangtze River Basin is the main production area of ​​rapeseed in my country. Although the rainfall is abundant, the precipitation is unevenly distribut...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 陆光远李春生
Owner HUBEI ENG UNIV