Preparation and application of indole derivative

A technology of indole derivatives and methanol, applied in the field of microbial herbicides, can solve problems such as endangering human health, environmental pollution, weed resistance, and endangering the safety of agricultural products, and achieve low product cost, good herbicidal activity, and short cycle Effect

Active Publication Date: 2018-07-20
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for a long time, the application of chemical pesticides has caused a series of problems such as environmental pollution and increased wee

Method used

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  • Preparation and application of indole derivative
  • Preparation and application of indole derivative
  • Preparation and application of indole derivative

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example 1: Indole derivatives of chlamydosporin type are shown in formula (1) (the Arabic numerals in the structure are the standard positions of carbon atoms).

[0023]

Example Embodiment

[0024] Example 2: Fermentation production, separation and purification of chlamydosporin indole derivatives:

[0025] 1) Fermentation culture

[0026] Strain culture: According to the conventional culture method of microorganisms, select a small amount of strains stored in agar-malt extract medium Fusarium chlamydosporum QJP1, inoculated on the surface of the PDA plate, cultured at 28°C for 3 days, as a strain for large-scale fermentation culture, ready for use.

[0027] Cut an appropriate amount of bacteria on the surface of the PDA plate, inoculate it into a sterilized Erlenmeyer flask containing rice culture medium, and let it stand for 30 days at room temperature. Sterilize by adding ethyl acetate and set aside.

[0028] The rice culture medium is 100 g / bottle of rice, 0.6 g / bottle of peptone, and 100 mL / bottle of artificial sea water.

[0029] 2) Separation and purification of compounds

[0030] The above-mentioned rice culture medium was ultrasonically extracted 3 times with eth...

Example Embodiment

[0033] Example 3: Herbicidal activity test

[0034] The agar powder mixing method established by Luo Xiaoyong et al. [Journal of Qingdao Agricultural University, 2007; 24(4): 267-270] is used to quickly determine the herbicidal activity of compounds, with high sensitivity, simple operation, short determination time, and good repeatability. , The characteristics of large-throughput and extensive screening, and easy to observe the growth status of indicating plants. Luo Xiaoyong et al. used this method to quickly determine the herbicidal activity of 40 garden plant leaves. Zhang Lijuan [Plant Protection, 2016; 42:63-66.] and Su Fansheng [Applied Chemistry, 2014;31:290-295.] Using barnyardgrass as the test weed, the corn stalk was quickly determined by the agar mixture method And the herbicidal activity of imidazole compounds. Zhang Yun[Journal of Microbiology, 2015;55(3):292-298.] and Shuai Li[Journal of Agricultural and Food Chemistry, 2014; 62(14):8997-9001.] Taking amaranthus ...

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Abstract

The invention discloses preparation and application of a chlamydosporin type indole derivative. The derivative has a structure shown as a formula (I), has better herbicidal activity, has 95.8 percentof growth inhibition rate on [Echinochloa crusgalli (L.) Beauv.] radicles at a concentration of 5 mug/mL and also has 81.8 percent of growth inhibition rate on amaranthus retroflexus coleoptiles as aconcentration of 10 mug/mL. Therefore, the derivative can be taken as a leading compound with an herbicidal effect or a new pesticide ingredient. The derivative shown as the formula (I) is preserved in the China Microbial Culture Collection Management Center on December 9, 2016, with the preservation number of CGMCC No. 13198 and is prepared by fermentation, culture, extraction and separation, andthe chemical structure of the derivative is identified by nuclear magnetic resonance and mass spectrometry and other technologies.

Description

technical field [0001] The invention relates to a microbial herbicide, in particular to the preparation and application of a chlamydosporin-like indole derivative. Background technique [0002] Weeds have always been a major problem that plagues agricultural production, affects crop yields, or disrupts ecological balance. barnyard grass [ Echinochloa crusgalli (L.) Beauv .] is the most important weed in paddy fields and one of the 18 kinds of malignant weeds in the world. It is widely distributed and highly competitive. It mainly relies on herbicides for control in production. Amaranthus retroflexus ( Amaranthus retroflexus ) is a common weed in farmland, orchard, roadside and wasteland, and often pollutes crop seeds. If it is not effectively controlled, the yields of corn, soybeans, spring wheat, rapeseed and vegetables will be significantly damaged. [0003] In recent years, chemically synthesized pesticides have been widely used to control weeds in crops. But fo...

Claims

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Application Information

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IPC IPC(8): C07D209/18A01N43/38A01P13/00C12P17/10C12R1/77
CPCA01N43/38C07D209/18C12P17/10
Inventor 肖琳杜丰玉周远明王兆富
Owner QINGDAO AGRI UNIV
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