Red algae polysaccharide compound bacterium agent as well as preparation method and application thereof

Abstract

The invention relates to the field of plant protection preparations and provides a red algae polysaccharide compound bacterium agent. The red algae polysaccharide compound bacterium agent is preparedfrom red algae polysaccharide hydrolysate, compound bacterium liquid and a Hoagland's nutritional solution, wherein the compound bacterium liquid comprises bacillus, and also comprises one or two of clostridium butyricum and lactobacillus plantarum; the compound bacterium liquid contains 1.0*10<8> to 1.0*10<9>CFU / mL of the bacillus. According to the red algae polysaccharide compound bacterium agent provided by the invention, the red algae polysaccharide hydrolysate and the compound bacterium liquid are compounded to be used as a bacterium-inhibition active substance and a good bacterium-inhibition effect is obtained; the Hoagland's nutritional solution can provide a stable living environment and nutrient substances of initial reproduction for the compound bacterium liquid. The red algae polysaccharide compound bacterium agent has the good bacterium-inhibition effect on plant pathogenic bacteria and is safe and environmentally friendly; the red algae polysaccharide compound bacterium agent is simple and convenient to operate, low-cost and easily popularized and applied in a large scale.

Description

technical field

[0001] The invention relates to the field of plant protection preparations, in particular to a red algal polysaccharide compound bacterial agent and a preparation method thereof, and the application of the red algal polysaccharide compound bacterial liquid in plant protection. Background technique

[0002] Seaweed polysaccharide is a kind of multi-component mixture, which is connected by different monosaccharide groups through glycosidic bonds. It is a general term for various high-molecular carbohydrates contained in seaweed cells and cells. Most of them contain sulfate groups, and most of them have high viscosity or coagulation. ability. Seaweed polysaccharides can be divided into red algal polysaccharides, green algal polysaccharides and algal polysaccharides according to their sources.

[0003] Red algae polysaccharide is a galactan sulfate extracted from red algae. Existing studies have shown that red algae polysaccharide has biological activities such ...

Examples

preparation example Construction

[0046] The present invention provides the preparation method of the red algae polysaccharide composite bacterial agent described in the above technical scheme, comprising the following steps:

[0047] S1. Mixing the red algae polysaccharide hydrolyzate and Hoagland's nutrient solution according to the mass volume ratio to obtain the red algae polysaccharide hydrolyzate solution;

[0048] S2. Mixing the composite bacterial solution and the red algal polysaccharide hydrolyzate solution according to the volume ratio to obtain the red algal polysaccharide composite microbial agent.

[0049] Specifically, the present invention mixes the red algae polysaccharide hydrolyzate and Hoagland's nutrient solution according to the mass volume ratio of 15-35g:90-120mL to obtain the red algae polysaccharide hydrolyzate solution; The volume ratio of the nutrient solution is 0.5-2:0.5-2 and the composite bacterial solution is mixed with the red algal polysaccharide hydrolyzate solution to obtai...

Embodiment 1

[0057] Take 150g altar seaweed and extract red algae polysaccharide according to the method described in Chinese patent CN201410642608.4 to obtain 135g red algae polysaccharide. Mix 135g of red algal polysaccharide with 15L of 5% acetic acid solution, ultrasonicate at 30kHz for 24 hours, and dry at 60°C until a low molecular weight red algal polysaccharide hydrolyzate with a moisture content of less than 15% is obtained. The red algal polysaccharide hydrolyzate The degree of polymerization is 3-8.

[0058] Take 13.5 g of the red algal polysaccharide hydrolyzate prepared above and mix it with 80 mL of Hoagland's nutrient solution to obtain 80 mL of red algal polysaccharide hydrolyzate solution with a mass volume fraction of 16.875%.

[0059] Take the number of live bacteria 1.5×10 8 CFU / g of Bacillus subtilis agent 200g, 0.2×10 8 CFU / g Clostridium butyricum 120g was dissolved in 320mL Hoagland's nutrient solution, and the total number of viable bacteria was 1.08×10 8 CFU / mL ...

Embodiment 2

[0062] Take 200g altar seaweed and extract red algae polysaccharide according to the method described in Chinese patent CN201410642608.4 to obtain 181g red algae polysaccharide. Mix 181g of red algal polysaccharide with 20L of 10% acetic acid solution by mass fraction, ultrasonicate at 35kHz for 48 hours, and dry at 65°C until a low molecular weight red algal polysaccharide hydrolyzate with a moisture content of less than 12% is obtained. The red algal polysaccharide hydrolyzate The degree of polymerization is 4-10.

[0063] Take 60 g of the red algal polysaccharide hydrolyzate prepared above and mix it with 200 mL of Hoagland's nutrient solution to obtain 200 mL of red algal polysaccharide hydrolyzate solution with a mass volume fraction of 30%.

[0064] Take the number of live bacteria 1.6×10 8 CFU / g of Bacillus subtilis agent 100g, 0.2×10 8 CFU / g Clostridium butyricum 100g and 0.5×10 8 CFU / g of Lactobacillus plantarum 100g was dissolved in 200mL Hoagland's nutrient solut...

technical field

[0001] The invention relates to the field of plant protection preparations, in particular to a red algal polysaccharide compound bacterial agent and a preparation method thereof, and the application of the red algal polysaccharide compound bacterial liquid in plant protection. Background technique

[0002] Seaweed polysaccharide is a kind of multi-component mixture, which is connected by different monosaccharide groups through glycosidic bonds. It is a general term for various high-molecular carbohydrates contained in seaweed cells and cells. Most of them contain sulfate groups, and most of them have high viscosity or coagulation. ability. Seaweed polysaccharides can be divided into red algal polysaccharides, green algal polysaccharides and algal polysaccharides according to their sources.

[0003] Red algae polysaccharide is a galactan sulfate extracted from red algae. Existing studies have shown that red algae polysaccharide has biological activities such ...

Examples

preparation example Construction

[0046] The present invention provides the preparation method of the red algae polysaccharide composite bacterial agent described in the above technical scheme, comprising the following steps:

[0047] S1. Mixing the red algae polysaccharide hydrolyzate and Hoagland's nutrient solution according to the mass volume ratio to obtain the red algae polysaccharide hydrolyzate solution;

[0048] S2. Mixing the composite bacterial solution and the red algal polysaccharide hydrolyzate solution according to the volume ratio to obtain the red algal polysaccharide composite microbial agent.

[0049] Specifically, the present invention mixes the red algae polysaccharide hydrolyzate and Hoagland's nutrient solution according to the mass volume ratio of 15-35g:90-120mL to obtain the red algae polysaccharide hydrolyzate solution; The volume ratio of the nutrient solution is 0.5-2:0.5-2 and the composite bacterial solution is mixed with the red algal polysaccharide hydrolyzate solution to obtai...

Embodiment 1

[0057] Take 150g altar seaweed and extract red algae polysaccharide according to the method described in Chinese patent CN201410642608.4 to obtain 135g red algae polysaccharide. Mix 135g of red algal polysaccharide with 15L of 5% acetic acid solution, ultrasonicate at 30kHz for 24 hours, and dry at 60°C until a low molecular weight red algal polysaccharide hydrolyzate with a moisture content of less than 15% is obtained. The red algal polysaccharide hydrolyzate The degree of polymerization is 3-8.

[0058] Take 13.5 g of the red algal polysaccharide hydrolyzate prepared above and mix it with 80 mL of Hoagland's nutrient solution to obtain 80 mL of red algal polysaccharide hydrolyzate solution with a mass volume fraction of 16.875%.

[0059] Take the number of live bacteria 1.5×10 8 CFU / g of Bacillus subtilis agent 200g, 0.2×10 8 CFU / g Clostridium butyricum 120g was dissolved in 320mL Hoagland's nutrient solution, and the total number of viable bacteria was 1.08×10 8 CFU / mL ...

Embodiment 2

[0062] Take 200g altar seaweed and extract red algae polysaccharide according to the method described in Chinese patent CN201410642608.4 to obtain 181g red algae polysaccharide. Mix 181g of red algal polysaccharide with 20L of 10% acetic acid solution by mass fraction, ultrasonicate at 35kHz for 48 hours, and dry at 65°C until a low molecular weight red algal polysaccharide hydrolyzate with a moisture content of less than 12% is obtained. The red algal polysaccharide hydrolyzate The degree of polymerization is 4-10.

[0063] Take 60 g of the red algal polysaccharide hydrolyzate prepared above and mix it with 200 mL of Hoagland's nutrient solution to obtain 200 mL of red algal polysaccharide hydrolyzate solution with a mass volume fraction of 30%.

[0064] Take the number of live bacteria 1.6×10 8 CFU / g of Bacillus subtilis agent 100g, 0.2×10 8 CFU / g Clostridium butyricum 100g and 0.5×10 8 CFU / g of Lactobacillus plantarum 100g was dissolved in 200mL Hoagland's nutrient solut...