A method for establishing a rat model for experimentally monitoring the process of malignant transformation of hepatocytes
A technology of transformation process and rat model, which is applied in the field of medical research, development and utilization, and can solve problems such as unclear relationships
Active Publication Date: 2020-06-12
AFFILIATED HOSPITAL OF NANTONG UNIV +1
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But at present, the relationship between HMGB3 and HCC is not yet clear
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[0044] The present invention will be further described below in conjunction with the accompanying drawings.
[0045] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, and are not intended to limit the present invention.
[0046] A method for establishing a rat model for experimentally monitoring the process of malignant transformation of hepatocytes, comprising the following steps:
[0047] (1) Animal grouping: select 40 Sprague-Durer rats, optionally, male Sprague-Durer rats of clean grade, 4-6 weeks old, and weighing 100-120 g, They were raised in a clean room with a 12-hour day and night cycle and a humidity of 55%. Ten rats were randomly selected as healthy control groups, and ...
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The invention discloses application of high mobility group box-3 (HMGB3) in monitoring a hepatic cell vicious transformation process, comprising the following steps: animal grouping, model preparation, preparation of paraffin section of rat liver tissue, H&E staining of rat liver, detection and calculation of mRNA expression level of the HMGB family in the rat liver cancer formation dynamic process by a Trizol method, and detection of protein expression level of the HMGB3 in the rat liver cancer formation process by an immunohistochemical technology. According to the invention, a rat liver cancer model is built, the hepatic cell vicious transformation is induced by 2-acetaminofluorene; discovered through RT-qPCR, by analyzing the mRNA level of the HMGB family in different stages of the liver cancer formation, the HMGB3 expression dynamically increases in the liver vicious transformation process; the immunohistochemical technology verifies that the expression of the HMGB3 protein levelin the liver cancer formation process is significantly increased; thus the HMGB3 can be applied to the dynamic monitoring of the hepatic cell vicious transformation process.
Description
technical field [0001] The invention relates to the technical field of medical research, development and utilization, in particular to a method for establishing a rat model for experimentally monitoring the malignant transformation process of liver cells. Background technique [0002] Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in the world. The occurrence and development of HCC is a complex process of multi-etiology, multi-gene, multi-step and multi-factor coordination, involving the activation of oncogenes and the inactivation of tumor suppressor genes. and the reactivation of some oncogenes in the embryonic period and other multiple signaling pathways and gene regulation. At present, the serological diagnostic marker of liver cancer is mainly AFP, but its effect is not satisfactory, which directly affects its clinical treatment. Therefore, finding specific and effective early markers is of great significance for the diagnosis and treatment o...
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IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/57438G01N33/68
Inventor 郑文杰董志珍姚敏姚登福方淼王理
Owner AFFILIATED HOSPITAL OF NANTONG UNIV