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Application of water-soluble realgar solid dispersoid in preparation of bone marrow hematopoietic stem/progenitor cell erythroid differentiation inducer

A technology of solid dispersion and bone marrow hematopoiesis, applied in the field of medicine, can solve problems such as large toxic and side effects, limited drug reports, etc.

Pending Publication Date: 2018-07-31
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, EPO has a large problem of toxic and side effects in clinical application.
With the exception of EPO, reports on other drugs are very limited

Method used

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  • Application of water-soluble realgar solid dispersoid in preparation of bone marrow hematopoietic stem/progenitor cell erythroid differentiation inducer
  • Application of water-soluble realgar solid dispersoid in preparation of bone marrow hematopoietic stem/progenitor cell erythroid differentiation inducer
  • Application of water-soluble realgar solid dispersoid in preparation of bone marrow hematopoietic stem/progenitor cell erythroid differentiation inducer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0045] The water-soluble realgar solid dispersions of Examples 1-10 were prepared by the following preparation methods. Only the composition of raw materials and the preparation process parameters were adjusted between different examples. For specific adjustments, see Table 1 (mentioned in Table 1). The specific preparation method is as follows: weighing a certain weight of realgar and macromolecule (and surfactant), stirring and mixing in a container to form materials, and then adding them into an extruder. The temperature in the extruder is set at 50-200° C., and the screw speed is 5-100 r / min. The material is extruded from the die hole of the machine head, and an orange-yellow solid product is obtained after cooling at room temperature.

[0046] Raw material composition and preparation process parameters of water-soluble realgar solid dispersion of table 1

[0047]

[0048]

experiment example 1

[0049] Experimental Example 1: Water Solubility Evaluation

[0050] The water-soluble realgar solid dispersion (e-As) of embodiment 5 is taken by weighing 4 S 4 ) 1.500g and realgar raw material medicine realgar (r-As 4 S 4 ) powder 100mg were dissolved in 10mL double distilled water (ddH 2 O), get e-As 4 S 4 and r-As 4 S 4 The aqueous dispersion, the appearance of the aqueous dispersion is as figure 1 shown.

[0051] Visible e-As 4 S 4 Rapidly soluble in ddH 2 O, realgar enters the water phase in a large amount, and the dissolution rate in water increases significantly, forming a yellow uniform colloidal solution; while r-As 4 S 4 Then it precipitates at the bottom and is difficult to dissolve in water.

[0052] Other embodiments also have similar phenomena, which will not be repeated here.

experiment example 2

[0053] Experimental example 2: e-As 4 S 4 DLS Analysis of Particle Size Distribution

[0054] Weigh the e-As of 20mg embodiment 4 4 S 4 powder, add 1.25mL ddH 2 0, make the dispersion liquid of suspension, draw 1mL above-mentioned dispersion liquid and be placed in cuvette, use Nano ZS90 nano particle size analyzer to test particle diameter, the result obtained is as follows figure 2 shown.

[0055] Depend on figure 2 It can be seen that the realgar nanoparticles e-As in the dispersion 4 S 4 The average hydrated particle size is about 470nm.

[0056] For the particle size distribution results of other examples dissolved in different media, refer to Experimental Example 6 of CN106236773A.

[0057] For other physical and chemical properties of the water-soluble realgar solid dispersion, refer to Experimental Example 1 to Experimental Example 8 of CN106236773A.

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Abstract

The invention discloses application of a water-soluble realgar solid dispersoid in preparation of a bone marrow hematopoietic stem / progenitor cell erythroid differentiation inducer. The realgar soliddispersoid is prpeared from the following raw materials in parts by weight: 1 part of realgar, 1 to 20 parts of macromolecule and 0 to 5 parts of surfactant. According to the application disclosed bythe invention, the situation that the water-soluble realgar solid dispersoid can induce bone marrow hematopoietic stem cells / progenitor cells to differentiate toward erythrocytes is found, so that accumulation of erythroid cell in bone marrow cells is promoted, and the reduction of the quantity of the erythrocytes caused by the bone marrow hematopoietic stem / progenitor cell erythroid differentiation is effectively alleviated, anemia caused by hematopoietic obstacle is improved, and simultaneously, the bone marrow cells can be protected from being killed by medicines.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of water-soluble realgar solid dispersion in the preparation of bone marrow hematopoietic stem / progenitor cell erythroid differentiation inducer. Background technique [0002] Bone marrow is the hematopoietic tissue in the human body, located in the medullary cavity of long bones and all bone marrow. There are two types of bone marrow in adults: red marrow and yellow marrow. Red bone marrow produces red blood cells, platelets and various white blood cells. Platelets have a hemostatic effect, and white blood cells can kill and inhibit various pathogens, including bacteria and viruses. Bone marrow mononuclear cells are a type of cells with a single nucleus in bone marrow cells, mainly including bone marrow hematopoietic stem / progenitor cells, monocytes and lymphocytes, among which bone marrow hematopoietic stem / progenitor cells can differentiate into red blood cells and ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/36A61K9/14A61P7/06A61P35/02A61P7/00A61P19/08A61P7/02A61P7/04
CPCA61P7/00A61P7/02A61P7/04A61P7/06A61P19/08A61P35/02A61K9/146A61K33/36C12N5/0641C12N2500/05C12N2506/11C12N2501/999C12N2506/1353
Inventor 许海燕刘健王涛韩冰孟洁温涛
Owner THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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