A porous microsphere cell scaffold with dual functions of antibacterial and osteogenesis and its preparation method

A technology of porous microspheres and cell scaffolds, which is applied in pharmaceutical formulations, pharmaceutical sciences, prostheses, etc., can solve problems such as low bioavailability, achieve simple and effective preparation methods, promote adhesion and proliferation, and have significant bacteriostatic activity. Effect

Active Publication Date: 2021-06-08
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the microspheres prepared in this way also exhibit good osteogenic activity, the inactivation of growth factors during the embedding process is often inevitable and the bioavailability is low.

Method used

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  • A porous microsphere cell scaffold with dual functions of antibacterial and osteogenesis and its preparation method
  • A porous microsphere cell scaffold with dual functions of antibacterial and osteogenesis and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) 2 g of poly(L-lactide-polyethylene glycol-poly(L-lactide) triblock copolymer with polyethylene glycol block molecular weight of 2000 and poly(L-lactide) block molecular weight of 20,000 is dissolved in 20ml Dichloromethane, add 0.05g Span 80, then add 2ml deionized water, ultrasonic 3 minutes under 200W to obtain water-in-oil (W 1 / O) Emulsion A;

[0034] (2) configuration concentration is 300ml of polyvinyl alcohol aqueous solution of 10mg / ml, adds 0.1g Tween 60, then under 500rpm stirring speed, emulsion A is joined in the polyvinyl alcohol aqueous solution that has added 0.1g Tween 60, Get water-in-oil-in-water (W 1 / O / W 2 ) double emulsion, the stirring time was continued for 4h, centrifuged after dichloromethane volatilized completely, the obtained microspheres were washed with deionized water, and freeze-dried to obtain biodegradable porous microspheres I (attached figure 1 );

[0035] (3) Prepare a dopamine aqueous solution with a concentration of 2 mg / ml...

Embodiment 2

[0039] (1) 2 g of poly(L-lactide-polyethylene glycol-poly(L-lactide) triblock copolymer with polyethylene glycol block molecular weight of 6000 and poly(L-lactide) block molecular weight of 50,000 is dissolved in 20ml Add 0.10 g of Span 80 to dichloromethane, then add 2 ml of deionized water, and ultrasonicate at 200 W for 3 minutes to obtain a water-in-oil (W1 / O) emulsion B;

[0040] (2) 300ml of gelatin aqueous solution with a concentration of 30mg / ml is added, 0.05g of Tween 60 aqueous solution is added, and then at a stirring speed of 500rpm, emulsion B is added into the gelatin aqueous solution of 0.05g Tween 60 to obtain oil-in-water Pack water (W 1 / O / W 2 ) double emulsion, continuously stirred for 6 hours, and centrifuged after dichloromethane volatilized completely, the obtained microspheres were washed with deionized water, and freeze-dried to obtain biodegradable porous microspheres IV;

[0041] (3) Prepare a dopamine aqueous solution with a concentration of 2 mg / ...

Embodiment 3

[0045] (1) 1 g of polycaprolactone-polyethylene glycol-polycaprolactone triblock copolymer with polyethylene glycol block molecular weight of 4000 and polycaprolactone block molecular weight of 30000 is dissolved in 20ml methylene chloride , add 0.10g Span 80, then add 2ml deionized water, and obtain water-in-oil (W 1 / O) Emulsion C;

[0046] (2) configuration concentration is 200ml of polyvinyl alcohol aqueous solution of 100mg / ml, adds the Tween 60 of 0.10g, then under 300rpm stirring speed, emulsion A is joined in the polyvinyl alcohol aqueous solution that has added 0.10g Tween 60, Get water-in-oil-in-water (W 1 / O / W 2 ) double emulsion, continuously stirred for 4 hours, centrifuged after dichloromethane volatilized completely, the obtained microspheres were washed with deionized water, and freeze-dried to obtain biodegradable porous microspheres VII;

[0047] (3) Prepare a dopamine aqueous solution with a concentration of 1 mg / ml, and adjust the pH value of the solutio...

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Abstract

A porous microsphere cell scaffold with dual functions of antibacterial and osteogenesis and a preparation method thereof relate to the field of biomaterials. In view of the fact that the existing bone-promoting materials are generally single in functionality, and the bone tissue engineering scaffolds with dual functions of antibacterial and osteogenesis are very limited, which is not conducive to the current situation of regeneration and repair of bone defects with risk of infection. The purpose of the present invention is to regenerate bone defects Repair provides a porous microsphere cell scaffold with dual functions of antibacterial and osteogenic differentiation. The microspheres are composed of porous microspheres of biodegradable aliphatic polyester-polyethylene glycol amphiphilic block copolymers and polydopamine, silver nanoparticles and hydroxyapatite sequentially loaded on the surface. Adhesion and proliferation, its loaded silver nanoparticles provide antibacterial activity, and its loaded hydroxyapatite provides osteogenic activity, it is an injectable bone defect regenerative repair material.

Description

technical field [0001] The invention relates to the field of biological materials, and relates to a bone regeneration repair material and a preparation method thereof, in particular to a porous microsphere cell scaffold with dual functions of antibacterial and osteogenic differentiation and a preparation method thereof. Background technique [0002] In recent years, the number of bone defects caused by tumor resection, trauma, infection and congenital malformations has increased day by day. Thousands of cases occur every year, and advanced biomedical materials are urgently needed to meet the relevant treatment needs. Compared with the shortcomings of bone grafting, bone tissue engineering provides a very promising repair strategy. However, there is a potential risk of local infection in the regenerative repair of bone defects, or the regenerative repair of infected bone defects (such as bone defects in some oral environments), which may bring severe challenges to clinical tr...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): A61L27/04A61L27/12A61L27/18A61L27/54A61L27/56A61L27/58
CPCA61L27/047A61L27/12A61L27/18A61L27/54A61L27/56A61L27/58A61L2300/104A61L2300/112A61L2300/404A61L2300/412A61L2400/06A61L2430/02
Inventor蔡晴魏鹏飞袁作楹杨小平黄丹妮
OwnerBEIJING UNIV OF CHEM TECH