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A kind of preparation method of multiple fluorescently labeled polystyrene microspheres

A technology of polystyrene microspheres and multiple fluorescence, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of narrow emission spectrum, easy fluorescence quenching, poor photostability, etc., achieve wide and continuous excitation spectrum, no There are potential safety hazards, and the raw materials are non-toxic and harmless

Active Publication Date: 2020-05-01
湖北新纵科病毒疾病工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing fluorescent substances have certain defects in the application process: 1. Poor photostability, narrow and discontinuous emission spectrum, wide and asymmetric emission spectrum, and uncontrollable emission spectrum; 2. Fluorescence is easy to quench and has a short lifespan; 3. , A small number of fluorescent substances have certain radioactivity and toxicity, and there are potential safety hazards

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] This embodiment provides a method for preparing multiple fluorescently labeled polystyrene microspheres, comprising the following steps:

[0032] S1, Preparation of surface carboxylated polystyrene microspheres by two-step seed swelling polymerization:

[0033] S11: Weigh 0.1 g of seed microspheres and add them to 30 mL of 0.25 wt % sodium dodecyl sulfate (SDS) aqueous solution, and ultrasonically disperse for 20 min at 200 W to obtain the first swollen product;

[0034] S12: Add another 0.1 g of cyclohexane to 30 mL of 0.25 wt % sodium dodecyl sulfate (SDS) aqueous solution, and ultrasonically disperse for 20 min at 200 W to obtain a second swollen product;

[0035] S13: Mix the above-mentioned first swelling product and the second swelling product according to the ratio of 1:1.2, swell at room temperature for 4 hours, add 0.1g dibenzoyl peroxide (BPO), 20g styrene, 20g divinylbenzene, 20g Toluene, 1.0 g of acrylic acid and 80 mL of 0.25 wt % sodium dodecyl sulfate (S...

Embodiment 2

[0044] This embodiment provides a method for preparing multiple fluorescently labeled polystyrene microspheres, comprising the following steps:

[0045] S1, Preparation of surface carboxylated polystyrene microspheres by two-step seed swelling polymerization:

[0046] S11: Weigh 0.3 g of the seed microspheres and add them to 60 mL of 0.25 wt % sodium dodecyl sulfate (SDS) aqueous solution, and ultrasonically disperse for 30 min at 300 W to obtain the first swollen product;

[0047] S12: Add another 0.3 g of cyclohexane to 60 mL of 0.25 wt % sodium dodecyl sulfate (SDS) aqueous solution, and ultrasonically disperse for 30 min at 300 W to obtain a second swollen product;

[0048]S13: Mix the first swelling product and the second swelling product at a ratio of 1:1.3, swell at room temperature for 6 hours, and sequentially add 0.3 g of dibenzoyl peroxide (BPO), 30 g of styrene, 30 g of divinylbenzene, 30 g of Toluene, 1.5g acrylic acid and 80mL 0.25wt% sodium dodecyl sulfate (SDS...

Embodiment 3

[0057] This embodiment provides a method for preparing multiple fluorescently labeled polystyrene microspheres, including the following steps:

[0058] S1, surface carboxylated polystyrene microspheres were prepared by two-step seed swelling polymerization:

[0059] S11: Weigh 0.5 g of seed microspheres and add them to 90 mL of 0.25Wt% sodium dodecyl sulfate (SDS) aqueous solution, and ultrasonically disperse for 40 min under the condition of 100-500 W to obtain the first swelling;

[0060] S12: take another 0.5g of cyclohexane and add it to 90mL of 0.25Wt% sodium dodecyl sulfate (SDS) aqueous solution, and ultrasonically disperse for 40min under the condition of 400W to obtain the second swelling;

[0061] S13: Mix the first swelling product and the second swelling product at a ratio of 1:1.4, swell at room temperature for 8 hours, and sequentially add 0.5 g of dibenzoyl peroxide (BPO), 40 g of styrene, 40 g of divinylbenzene, 40 g of Toluene, 2.0g acrylic acid and 80mL 0.25...

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Abstract

The invention relates to a preparation method of multiple fluorescently-labeled polystyrene microspheres, and the method comprises the steps of preparing surface-carboxylated polystyrene microspheresby two-step seed swelling polymerization, and combining the polystyrene microspheres with quantum dots to prepare quantum dot fluorescent microspheres, coupling carboxyl groups on the surface of the quantum dot fluorescent microspheres with phycoerythrin to obtain the multiple fluorescently-labeled polystyrene microspheres. The method has the advantages of being simple and low in raw material price, the prepared polystyrene microspheres have a stable structure, by use of double fluorescent labeling of the quantum dots and the phycoerythrin, the multiple fluorescently-labeled polystyrene microspheres have the advantages of remarkable performance characteristics, high safety factors in the preparation process, and no safety hazard to the human body.

Description

technical field [0001] The invention relates to the technical field of polymer microsphere material preparation, in particular to a preparation method of multiple fluorescently labeled polystyrene microspheres. Background technique [0002] Polystyrene fluorescent microspheres are uniform microspheres dyed with fluorescent reagents. Fluorescent microspheres refer to solid particles with diameters ranging from nanometers to micrometers, loaded with fluorescent substances, and excited by external energy to fluoresce. Its shape can be any shape, and the typical shape is spherical. The carriers of fluorescent microspheres are mostly organic or inorganic polymer materials. It has a relatively stable morphological structure and luminescent behavior, and is less affected by external conditions such as solvents, heat, electricity, and magnetism than pure fluorescent compounds. As a new type of carrier material, it has been widely used in the field of biomedicine. With the in-dept...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F212/08C08F212/36C08F220/06C08L25/06C08L89/00G01N21/64
CPCC08F212/08C08F212/36C08L25/06G01N21/64C08F220/06C08L89/00
Inventor 王华林张涛张科登
Owner 湖北新纵科病毒疾病工程技术有限公司
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