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Porcine erythrocyte ecr1-like membrane-bound peptide, polyclonal antibody, preparation method and application

An ecr1-like, polyclonal antibody technology, applied in receptor/cell surface antigen/cell surface determinant, anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, chemical instruments and methods, etc. Solve problems such as unclear related mechanisms, effects on immune function affecting the distribution of ECR1-like membranes, and limited sample data

Active Publication Date: 2021-11-23
SHANXI AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But in general, the detection index of porcine erythrocyte immune function is single and the sample data are limited, so the relevant mechanism of porcine erythrocyte immunity theory is still unclear
[0003] The problem in the prior art is that it is not clear about the membrane-bound peptide sequence that plays a role in porcine erythrocyte ECR1-like, which affects the subsequent in-depth research on related issues such as the distribution of ECR1-like membranes and its impact on immune function

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  • Porcine erythrocyte ecr1-like membrane-bound peptide, polyclonal antibody, preparation method and application
  • Porcine erythrocyte ecr1-like membrane-bound peptide, polyclonal antibody, preparation method and application
  • Porcine erythrocyte ecr1-like membrane-bound peptide, polyclonal antibody, preparation method and application

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Embodiment Construction

[0024] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but should not be construed as a limitation of the present invention. The experimental methods in the following examples, unless otherwise specified, are conventional methods, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0025] The sources of experimental materials in the following examples are as follows:

[0026] (1) Experimental animals

[0027] Healthy Landrace pigs, healthy New Zealand white rabbits (Zhichao Farm, Beiwang Village, Taigu County), GFP-E.coli (produced by transferring a plasmid containing GFP into E.coli according to a conventional method).

[0028] Pichia pastoris strain X-33, expression plasmid pwPICZalpha, and Escherichia coli competent Trans10 cells (E.coli) were purchased from Beijing Quanshijin Biotechnology Co., Ltd.

[0029] ...

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Abstract

The invention belongs to the technical field of porcine erythrocyte ECR1-like membrane-bound peptide antibody, and in particular relates to a porcine erythrocyte ECR1-like membrane-bound peptide, polyclonal antibody, preparation method and application. The amino acid sequence of the porcine erythrocyte ECR1-like membrane-bound peptide is shown in SEQ ID NO.1, which is ISANTVILFWFTCLFVQLY, and the corresponding nucleotide sequence is shown in SEQ ID NO.2, which is ata agt gcc aac acg gta atc ​​cta ttc tgg ttt act tgt ctt ttt gtt caa ctatac. The invention successfully prepares the specific polyclonal antibody of the porcine erythrocyte ECR1-like membrane-bound peptide with immunological activity, and provides a research tool for further studying the molecular mechanism of porcine ECR1-like molecular immunology function.

Description

technical field [0001] The invention belongs to the technical field of porcine erythrocyte ECR1-like membrane-bound peptide antibody, and in particular relates to a porcine erythrocyte ECR1-like membrane-bound peptide, polyclonal antibody, preparation method and application. Background technique [0002] Early studies on immune rosettes measured the rosette rates of C3b receptors and immune complexes on the erythrocytes of Yorkshire × Changbai and Jinhua hybrid pigs, and the average values ​​were 8.14±1.25 and 4.64±0.84 respectively; the red blood cell immune function of Yunnan breed pigs, the C3bRR rate The ICR rate was 17.2±4.20, and the ICR rate was 11.2±3.27, and there was no significant difference between the two (P>0.05). Researchers conducted a comparative study on the red blood cell immune function of black-and-white dairy cows, pigs, and Tibetan dogs in Xining area. The results proved that the red blood cell membranes of the three tested animals all had C3bR, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/705C07K16/28
CPCC07K14/70525C07K16/2821
Inventor 尹伟李宏全范阔海孙娜孙耀贵
Owner SHANXI AGRI UNIV