Quantum dot immunochromatography detection card and detection method for detecting ibuprofen through double-antibody sandwich competitive immunoassay
A double-antibody sandwich and immunochromatography technology, which is used in measurement devices, analytical materials, material excitation analysis, etc., can solve the problems of inability to uniformly prepare signal substances, insufficient stability, low detection signal strength and sensitivity, and achieve activity protection. Beneficial effect, good colloidal properties and kinetic properties, and the effect of short detection time
Active Publication Date: 2018-08-14
SHAOGUAN COLLEGE
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Problems solved by technology
The "binary system immunocompetition method" has the following disadvantages: 1. It is necessary to label the signal substance for each detection antibody separately, so that the signal substance cannot be prepared uniformly; 2. The detection antibody is in the liquid phase and has insufficient stability; 3. The detection signal intensity and less sensitive
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[0029] see figure 1 , which is the quantum dot immunochromatography detection card for the detection of ibuprofen by the double-antibody sandwich immunocompetitive method of this embodiment, including a PVC liner, and detection cards that are sequentially adhered to the PVC liner and partially overlapped between adjacent ones. Antigen release pad, quantum dot probe release pad, nitrocellulose membrane (NC membrane) and water absorption pad; The detection antigen release pad comprises the detection antigen formed by OVA and ibuprofen coupling; The quantum dot probe releases The pad contains quantum dot probes labeled with anti-OVA antibody; the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is immobilized with anti-ibuprofen antibody, and the quality control line is immobilized with OVA antigen.
[0030] Specifically, the preparation method of the detection antigen release pad is as follows: a glass cellulose membrane wi...
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The invention relates to a quantum dot immunochromatography detection card and a detection method for detecting ibuprofen through double-antibody sandwich competitive immunoassay. The detection card comprises a lining plate, a detection antigen release pad, a quantum dot probe release pad, a chromatography membrane and a water absorption pad. The detection antigen release pad comprises a detectionantigen formed by coupling ovalbumin and the ibuprofen; the quantum dot probe release pad comprises a quantum dot probe labelled with an anti-ovalbumin antibody; the chromatography membrane is provided with a detection line and a quality control line; the detection line fixes the anti-ibuprofen antibody; the quality control line fixes the ovalbumin antigen. The detection antigen is dissolved in adetection system from the release pad; the detection antigen, the detection antibody and a labelled antibody form a double-antibody sandwich immune complex for producing a fluorescence detection signal. Under the detection reference action of the quality control line, whether the ibuprofen is contained in a sample or not is judged according to the fluorescence signal on the detection line, so that the signal strength and the stability of detection are improved, and the detection sensitivity and the quantitative precision are improved.
Description
technical field [0001] The invention relates to the field of food and drug safety detection, in particular to a method of applying immunochemical detection technology to the detection of illegally added chemicals in health care products and Chinese patent medicines, especially the quantum dot immunochromatography for the detection of ibuprofen by the double-antibody sandwich immunocompetition method Test card and test method. Background technique [0002] The chemical name of ibuprofen is 2-methyl-4-(2-methylpropyl)phenylacetic acid, which belongs to non-steroidal anti-inflammatory drugs, has anti-inflammatory, analgesic and antipyretic effects, and is used for rheumatoid arthritis , Adhesive spondylitis, non-inflammatory joint pain, arthritis, non-articular rheumatism, pain caused by non-articular inflammation, various neuralgia, cancer pain, post-traumatic pain and fever caused by various inflammations, etc., but There are certain side effects, which are clinically prescr...
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Login to View More IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor郭杰标章仕杰江雪颜张鹏钟林涛黄钻元朱哲吴蓝洁
OwnerSHAOGUAN COLLEGE