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Fermentation culture medium for high-yield production of fatty acids through Thraustochytrids, and applications thereof

A technology of fermentation medium and thraustochytrium, which is applied in the field of microorganisms, can solve the problems of unsatisfactory fatty acid production, achieve the effects of improving raw material utilization efficiency, facilitating large-scale fermentation production, and significantly increasing yield

Inactive Publication Date: 2018-08-21
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, thraustochytrium is fermented and cultured on medium containing glucose, and the yield of fatty acid is not ideal.

Method used

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  • Fermentation culture medium for high-yield production of fatty acids through Thraustochytrids, and applications thereof
  • Fermentation culture medium for high-yield production of fatty acids through Thraustochytrids, and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] A thraustochytrium high-yield fatty acid fermentation medium (abbreviated as MZ) is made by the following method: weigh 60g of glycerol, 1.5g of peptone, 1g of yeast extract, 0.25g of potassium dihydrogen phosphate, and 33g of artificial sea salt. Add ultrapure water to 1000ml, stir evenly, sterilize at 115°C for 21 minutes, cool to room temperature, and obtain.

Embodiment 2

[0023] A thraustochytrium high-yield fatty acid fermentation medium is prepared by the following method: Weigh 45g of glycerin, 2g of peptone, 0.5g of yeast extract, 0.3g of potassium dihydrogen phosphate, and 25g of sea salt, and add ultrapure water to 1000ml, stir well, sterilize at 115°C for 21 minutes, cool to room temperature, ready to serve.

Embodiment 3

[0025] A thraustochytrium high-yield fatty acid fermentation medium is made by the following method: Weigh 65g of glycerin, 1g of peptone, 1.5g of yeast extract, 0.2g of potassium dihydrogen phosphate, and 40g of sea salt, add ultrapure water to 1000ml, stir well, sterilize at 115°C for 21 minutes, cool to room temperature, ready to serve.

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Abstract

The invention discloses a fermentation culture medium for high-yield production of fatty acids through Thraustochytrids, and applications thereof. The preparation method of the fermentation culture medium comprises: weighing 45-65 g of glycerol, 1-2 g of peptone, 0.5-1.5 g of a yeast extract, 0.2-0.3 g of potassium dihydrogen phosphate and 25-40 g of artificial sea salt according to a certain ratio, adding ultrapure water to achieve a volume of 1000 ml, uniformly stirring, sterilizing for 21 min at a temperature of 115 DEG C, and cooling to a room temperature to obtain the product. According to the present invention, the production cost is saved by using the glycerol; compared to the original culture medium, the fermentation culture medium of the present invention has the significantly-increased total fatty acid yield, the significantly-increased saturated fatty acid yield and the significantly-increased DHA content, and improves the utilization rate of the raw material; and the fermentation culture medium has characteristics of simple fermentation condition, convenient operation, low production cost, easy large-scale fermentation and energy saving.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a thraustochytrium high-yield fatty acid fermentation medium and application thereof. Background technique [0002] Thraustochytrids are heterotrophic fungi-like marine protists that are widely distributed. Because the cells of Thraustochytrium can accumulate a large amount of fatty acids, which can reach more than 50% of the biomass content, including saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids. Among them, saturated fatty acids and monounsaturated fatty acids are the main raw materials for biodiesel synthesis. As the main component of cell membrane phospholipids, polyunsaturated fatty acids are an indispensable nutrient for human health, especially DHA (docosahexenoic acid). DHA can assist the normal development of brain cells and retina, while DHA also has the effect of preventing cardiovascular disease, hyperlipidemia and anti-inflammatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
CPCC12P7/6427
Inventor 汪光义赵月王秋珍张赛叶会科姚力芬
Owner TIANJIN UNIV
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