Concentration-gradient fluorescent calibration piece for laser microarray chip scanner

Abstract

The invention provides a concentration-gradient fluorescent calibration piece for a laser microarray chip scanner. The concentration-gradient fluorescent calibration piece comprises a glass substrateand an organic fluorescence mixed liquor, wherein the organic fluorescence mixed liquor is uniformly distributed on the glass substrate in a lattice manner and comprises an Alexa Fluor 488 fluorescentdye, a Cy3-dCTP fluorescent dye and a diluent, the diluent is a phosphate buffer liquor, a TE buffer liquor or an aqueous liquor, and the surface of the glass substrate is chemically modified or coated with a polymer piece. The lattice of the organic fluorescence mixed liquor on the glass substrate comprises 1-8 branch lattices, each branch lattice is arranged on the glass substrate in a uniformdistribution manner, organic fluorescence mixed liquor droplets of each branch lattice are arranged in A rows* B lines, A ranges from 20 to 40, and B ranges from 5 to 20. The concentration-gradient fluorescent calibration piece has the beneficial effects that the concentration-gradient fluorescent calibration piece is simple to produce, short in production period and low in cost and has outstanding detection effect.

Description

technical field [0001] The invention relates to the technical field of biochip production equipment, in particular to a concentration gradient fluorescence calibration sheet for a laser microarray chip scanner for calibrating the linear range, sensitivity and resolution of blue and green dual laser microarray chip scanners. Background technique [0002] With the rapid development of biotechnology, the combination of electronic technology and biotechnology has produced a brother product of semiconductor chips - biochips, which will bring a profound revolution to our lives. This revolution will make an immeasurable contribution to the sustainable development of the world. my country's biochip research began between 1997 and 1998. Although it started late, technology and industry developed rapidly, achieving a breakthrough from scratch, and gradually growing. Biochips have moved from the stage of technology research and product development to In the stage of technology applicat...

AI Technical Summary

Problems solved by technology

Making calibration sheets with inorganic fluorescent materials is complicated, time-consuming, and costly, while making calibration sheets with organic fluorescent materials is simple, quick, and low-cost

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