Concentration-gradient fluorescent calibration piece for laser microarray chip scanner

A microarray chip, concentration gradient technology, applied in fluorescence/phosphorescence, instruments, scientific instruments, etc., can solve the problems of complicated operation, time-consuming and high cost of calibration chips, and achieve simple and fast production methods, low cost, and high-quality production materials. Instrument-less effect

Inactive Publication Date: 2018-08-24
成都博奥晶芯生物科技有限公司
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Problems solved by technology

Making calibration sheets with inorganic fluorescent materials is complicated, time-consuming, and costly, while making calibration sheets with organic fluorescent materials is simple, quick, and low-cost

Method used

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  • Concentration-gradient fluorescent calibration piece for laser microarray chip scanner
  • Concentration-gradient fluorescent calibration piece for laser microarray chip scanner
  • Concentration-gradient fluorescent calibration piece for laser microarray chip scanner

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Embodiment Construction

[0018] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0019] An embodiment of the present invention: preparation of 31 concentration (row) × 10 column gradient calibration sheet

[0020] A concentration gradient fluorescence calibration sheet for a laser microarray chip scanner, the calibration sheet is a glass substrate with a chemically modified surface or a glass substrate coated with a polymer film on the surface, and its size is 75.6 × 25mm. The organic fluorescent mixed solution is arranged in a dot matrix on the surface of the sheet, and the organic fluorescent mixed solution includes Alexa Fluor 488 fluorescent dye, Cy3-dCTP fluorescent dye a...

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Abstract

The invention provides a concentration-gradient fluorescent calibration piece for a laser microarray chip scanner. The concentration-gradient fluorescent calibration piece comprises a glass substrateand an organic fluorescence mixed liquor, wherein the organic fluorescence mixed liquor is uniformly distributed on the glass substrate in a lattice manner and comprises an Alexa Fluor 488 fluorescentdye, a Cy3-dCTP fluorescent dye and a diluent, the diluent is a phosphate buffer liquor, a TE buffer liquor or an aqueous liquor, and the surface of the glass substrate is chemically modified or coated with a polymer piece. The lattice of the organic fluorescence mixed liquor on the glass substrate comprises 1-8 branch lattices, each branch lattice is arranged on the glass substrate in a uniformdistribution manner, organic fluorescence mixed liquor droplets of each branch lattice are arranged in A rows* B lines, A ranges from 20 to 40, and B ranges from 5 to 20. The concentration-gradient fluorescent calibration piece has the beneficial effects that the concentration-gradient fluorescent calibration piece is simple to produce, short in production period and low in cost and has outstanding detection effect.

Description

technical field [0001] The invention relates to the technical field of biochip production equipment, in particular to a concentration gradient fluorescence calibration sheet for a laser microarray chip scanner for calibrating the linear range, sensitivity and resolution of blue and green dual laser microarray chip scanners. Background technique [0002] With the rapid development of biotechnology, the combination of electronic technology and biotechnology has produced a brother product of semiconductor chips - biochips, which will bring a profound revolution to our lives. This revolution will make an immeasurable contribution to the sustainable development of the world. my country's biochip research began between 1997 and 1998. Although it started late, technology and industry developed rapidly, achieving a breakthrough from scratch, and gradually growing. Biochips have moved from the stage of technology research and product development to In the stage of technology applicat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/645
Inventor 牟禹郭涛叶东刘晓瑜宋云鹏李若然
Owner 成都博奥晶芯生物科技有限公司
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