Producing method of micromanipulation holding needle

A manufacturing method and a micromanipulation technique, which are applied in the field of the manufacture of micromanipulation holding needles, can solve the problems of porcine oocyte damage, inability to fix, and the holding needles are expensive, and achieve a simple and fast manufacturing method and reduce The effect of production cost

CN104099239AActive Publication Date: 2014-10-15INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2014-10-15

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Abstract

The invention discloses a producing method of a micromanipulation holding needle. The producing method comprises the steps as follows: (1) heating and then stretching a glass capillary tube, and breaking the glass capillary tube in a position where the outer diameter is in a range of 150-170 mu m to obtain a tube body connected with a tip part; (2) heating the tip part of the tube body to enable the inner diameter of the tip part to shrink to be in a range of 40-50 mu m; (3) horizontally placing the tube body treated in Step (2) and a heat source, placing the tube body above the heat source, and enabling the distance between the tip of the tube body and the heat source to be in a range of 2-3 mm in the horizontal direction; heating the tube body until the tip part of the tube body is bent downwards by 20-30 degrees; and then realizing production of the micromanipulation holding needle. The producing method is simple and rapid; according to the holding needle produced with the producing method, the producing cost of the micromanipulation holding needle can be effectively reduced; the outer diameter of the holding needle produced with the method is in the range of 150-170 mu m, so that an oocyte and an embryo of a pig can be fixed effectively.
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Description

technical field

[0001] The invention relates to a method for manufacturing a micromanipulation holding needle, which belongs to the technical field of micromanipulation tools. Background technique

[0002] Microneedles are one of the necessary tools for making transgenic animals, somatic cell nuclear transfer animals and chimeric animals. The smooth and flat operating needle can not only improve the operating efficiency, reduce the damage to oocytes and embryos, but also help the recovery of embryos. The current commercially produced holding needles have small inner and outer diameters, which cannot be effectively fixed when used in pig somatic cell nuclear transfer operations and embryo manipulations, and because of their small inner diameters, they are easy to damage pig eggs when the pressure is high. mother cell damage. In addition, commercial fixed needles are more expensive, which increases the cost of micromanipulation. However, it is necessary to purchase an expen...

Examples

Embodiment 1

[0024] Embodiment 1, the making of holding fixed needle for micromanipulation

[0025] The manufacturing method of the micromanipulation holding needle provided by the present invention comprises the following steps:

[0026] (1) stretching: such as image 3 As shown, fix the BJ-40 capillary glass tube (outer diameter 1.0mm, inner diameter 0.8mm, length 100mm) with both hands, rotate and heat the middle part on the outer flame of the alcohol lamp, feel the melting force and stretch quickly, and leave the outer flame of the alcohol lamp at the same time flame. Select a part with an outer diameter of 150-170 μm, and use a small grinding wheel to cut off one end.

[0027] Such as figure 1 and figure 2 As shown, the outer diameter of the capillary glass tube before stretching is 1.0 mm, and the inner diameter is 0.8 mm; after stretching, the outer diameter of the thinned part is 150-170 μm, and the length is 1-2 cm. If the part with an outer diameter of 150-170 μm cannot be ...

Embodiment 2

[0032] Example 2. Fixing porcine oocytes with micromanipulation needles

[0033] Utilize the holding pins and commercialized holding pins prepared by the manufacturing method provided by the present invention to respectively fix porcine oocytes for micromanipulation, and compare the experimental results:

[0034] Figure 7 Photographs of micromanipulation of porcine oocytes during immobilization using commercially available holding pins, courtesy of Figure 7 As can be seen, Figure 7 (b) and Figure 7 The needle insertion operation in (c) causes the oocyte to slide away from the holding needle, where the arrow in the figure is the direction of sliding; Figure 8 The micromanipulation provided by the present invention holds fixed needle and fixes the photo of porcine oocyte to carry out micromanipulation, by Figure 8 (b) and Figure 8 (c) It can be seen that inserting the needle from the side can make the oocyte rotate in situ, but it will not break away from the holding...