Producing method of micromanipulation holding needle

Abstract

The invention discloses a producing method of a micromanipulation holding needle. The producing method comprises the steps as follows: (1) heating and then stretching a glass capillary tube, and breaking the glass capillary tube in a position where the outer diameter is in a range of 150-170 mu m to obtain a tube body connected with a tip part; (2) heating the tip part of the tube body to enable the inner diameter of the tip part to shrink to be in a range of 40-50 mu m; (3) horizontally placing the tube body treated in Step (2) and a heat source, placing the tube body above the heat source, and enabling the distance between the tip of the tube body and the heat source to be in a range of 2-3 mm in the horizontal direction; heating the tube body until the tip part of the tube body is bent downwards by 20-30 degrees; and then realizing production of the micromanipulation holding needle. The producing method is simple and rapid; according to the holding needle produced with the producing method, the producing cost of the micromanipulation holding needle can be effectively reduced; the outer diameter of the holding needle produced with the method is in the range of 150-170 mu m, so that an oocyte and an embryo of a pig can be fixed effectively.

Description

technical field [0001] The invention relates to a method for manufacturing a micromanipulation holding needle, which belongs to the technical field of micromanipulation tools. Background technique [0002] Microneedles are one of the necessary tools for making transgenic animals, somatic cell nuclear transfer animals and chimeric animals. The smooth and flat operating needle can not only improve the operating efficiency, reduce the damage to oocytes and embryos, but also help the recovery of embryos. The current commercially produced holding needles have small inner and outer diameters, which cannot be effectively fixed when used in pig somatic cell nuclear transfer operations and embryo manipulations, and because of their small inner diameters, they are easy to damage pig eggs when the pressure is high. mother cell damage. In addition, commercial fixed needles are more expensive, which increases the cost of micromanipulation. However, it is necessary to purchase an expen...

AI Technical Summary

Problems solved by technology

The current commercially produced holding needles have small inner and outer diameters, which cannot be effectively fixed when used in pig somatic cell nuclear transfer operations and embryo manipulations, and because of their small inner diameters, they are easy to damage pig eggs when the pressure is high. mother cell damage

In addition, commercial fixed needles are more expensive, which increases the cost of micromanipulation

However, if you use a needle puller to make a holding needle yourself, you need to purchase an expensive needle puller

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