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Method for separating mycose from glucose

A separation method, glucose technology, which is applied in the field of separation of trehalose and glucose, can solve the problems of high cost and difficult separation, and achieve the effect of low cost, high stability, and simple and fast preparation method

Inactive Publication Date: 2013-05-22
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to address the deficiencies in the prior art, and propose a cheap and efficient method for separating trehalose and glucose to solve the problems of difficult separation and high cost in the current production process.

Method used

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  • Method for separating mycose from glucose
  • Method for separating mycose from glucose
  • Method for separating mycose from glucose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Preparation of trehalose and glucose mixture

[0031] With 20% maltose as the substrate, trehalose synthase catalyzes maltose to prepare a mixture of trehalose and glucose at 25°C and pH 7.0. The amount of trehalose synthase added is 10% of the mass of the maltose substrate. There is 30% maltose remaining in the solution; then treat the above-mentioned mixed solution with glucoamylase, the amount of glucoamylase added is 5‰ of the remaining maltose mass, at 60°C, pH is controlled at 4.0~5.0, and the rest can be dissolved after 5 hours of reaction All the miscellaneous sugars are hydrolyzed into glucose, so that the above mixed solution only contains trehalose and glucose. At this time, the reaction solution contains 75% trehalose and 25% glucose. .

[0032] (2) Preparation of nano gold

[0033] Nanogold was prepared by reducing chloroauric acid with sodium citrate. First, all glassware needs to be soaked in aqua regia to remove residual reducing substances in the g...

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Abstract

The invention belongs to the technical field of the sugar industry and discloses a method for separating mycose from glucose. According to the method, nano-gold is as a catalyst and the mixed liquid of mycose and glucose is as a substrate, wherein the nano-gold catalyzes glucose in the mixed liquid to generate gluconic acid; and then the gluconic acid is separated from the mycose through a chromatographic process. The glucose is catalyzed by the nano-gold to generate the gluconic acid, and the sugar-to-sugar separation applied to mycose and glucose is transformed into sugar-to-acid separation, so that the separation efficiency is greatly improved; besides, the method is simple and quick, wide in raw material source and low in cost; therefore, the method is an efficient new process for separating mycose from glucose.

Description

technical field [0001] The invention belongs to the technical field of sugar industry, in particular to a method for separating trehalose and glucose. Background technique [0002] Trehalose is a stable non-reducing disaccharide, easily soluble in water, and its sweetness is equivalent to 45% of that of sucrose. It is composed of two glucopyranose molecules connected by α,α-1,1-glycosidic bonds. Since the reducing groups of the two molecules of glucose are used to form bonds, and the energy level of the glycosidic bonds is relatively high, it is abnormal It is stable and should not be damaged under the action of acid-base, oxidation, high temperature and general enzymes (except a few specific trehalases). As a functional sugar, it has attracted more and more attention in food, biomedicine, agriculture, health care products, cosmetics, etc. with its unique biological protection ability, and has a broad market prospect. [0003] The trehalose preparation methods currently be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/04C07H1/06
Inventor 唐飞黄和江凌李霜李燕萍
Owner NANJING UNIV OF TECH
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