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Preparation method of lipidosome and method for producing lipidosome adjuvant

A lipid and system technology, applied in the field of biomedicine, achieves the effects of good protein encapsulation capacity and immune response level, simple operation, and easy mass preparation

Inactive Publication Date: 2018-08-28
上海络祥基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the field of using liposomes for vaccines in China.

Method used

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  • Preparation method of lipidosome and method for producing lipidosome adjuvant
  • Preparation method of lipidosome and method for producing lipidosome adjuvant
  • Preparation method of lipidosome and method for producing lipidosome adjuvant

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Embodiment Construction

[0026]Weigh hydrogenated soybean lecithin HSPC, cholesterol CHOL, 1,2-dioleoyloxytrimethylammonium chloride DOTAP-Cl, vitamin VE, according to the ratio of mass fraction 70~90:0~30:10~20:0.5 Make mixture, 2) the mixture described in step 1 is dissolved in chloroform / methanol solvent to obtain the organic solution of lipid for subsequent use, and described chloroform / methanol solvent is 24: 72: 4-5.1 by volume ratio chloroform, methyl alcohol and supernatant 3) Weigh the water-soluble powder solid support and place it in a 250ml round bottom flask, then add the organic solution of the above-mentioned lipids, stir evenly, and place it on a rotary evaporator under the condition of constant temperature in an ice / water bath to rotate Evaporate the organic solvent, wherein the quality of the solid support is 5 times the quality of the mixture, including one or more of lactose, sucrose, mannitol, sodium chloride; 4) take off the round bottom flask, take out the solid The sample, name...

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Abstract

The invention relates to a preparation method of lipidosome and application of the lipidosome as a vaccine immunologic adjuvant. The invention provides a lipidosome preparation method. The preparationmethod comprises the following steps: (1) weighing hydrogenated soy phosphatidylcholine (HSPC), cholesterol (CHOL), 1,2-dioleoyloxytrimethylammonium chloride (DOTAP-Cl), and vitamin (VE), and mixinginto a mixture according to a weight ratio of (70 to 90): (0 to 30): (10 to 20): 0.5; (2) dissolving the mixture in step 1 in a chloroform / methanol solvent to obtain lipoid organic solution for standby use, wherein the chloroform / methanol solvent is prepared from chloroform, methanol and ultrapure water in a volume ratio of 24: 72: (4 to 5); and (3) weighing soluble powdery solid holder, placing in a 250ml round-bottom flask, adding the lipoid organic solution, uniformly stirring, placing on a rotary evaporator under a condition of icy / water bath constant-temperature, removing the organic solvent, wherein the weight of the solid holder is five times the weight of the mixture, and the solid holder is prepared from one or more of lactose, saccharose, mannitol and sodium chloride. The preparation method is simple in process, low in cost and suitable for the mass culture of lipidosome and applicable to the vaccine immunologic adjuvant.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a preparation method of liposome and its application as a vaccine immune adjuvant. Background technique [0002] Vaccination is a very effective way to prevent diseases, but some traditional vaccines can cause side effects to varying degrees. In recent years, with the continuous deepening of immunology research and the rapid development of genetic engineering technology, the research of new vaccines such as live vector vaccines, DNA vaccines and protein vaccines has made gratifying progress. These new vaccines have high purity and strong specificity, but their immunogenicity is weak, and the immune response induced by the body is not strong enough. Therefore, the use of safe and effective adjuvants to enhance the vaccine effect has become an urgent problem to be solved. [0003] The only adjuvants officially approved for use in human vaccines are aluminum adjuvants (mainly composed of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/12A61P37/04A61P31/20
CPCA61K39/39A61K39/12A61K2039/55555A61K2039/575C12N2710/20034
Inventor 张宇
Owner 上海络祥基因科技有限公司
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