Establishing method of tetrandra and poria decoction fingerprint atlas and fingerprint atlas

A technique of fingerprinting and establishing methods, which is applied in the field of traditional Chinese medicine analysis, can solve the problems of many components, single quality control, and few indicators, and achieve the effects of objective conclusion, control of preparation quality, and convenient operation

Active Publication Date: 2018-08-28
CHINA RESOURCES SANJIU MEDICAL & PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved by the present invention is to overcome that the traditional Chinese medicine compound preparation in the prior art basically only has one or two index components in content control, the quality control is single, the index is few, and it is difficult to fully reflect that there are many medicinal flavors and many components. , the defects of the quality status of the composition of Fangji Fuling Decoction with many targets, thereby providing the method for establishing the fingerprint of Fangji Poria Decoction and the fingerprint

Method used

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  • Establishing method of tetrandra and poria decoction fingerprint atlas and fingerprint atlas
  • Establishing method of tetrandra and poria decoction fingerprint atlas and fingerprint atlas
  • Establishing method of tetrandra and poria decoction fingerprint atlas and fingerprint atlas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1. Preparation of Fangji Fuling Decoction Composition

[0039] Weigh the following decoction pieces in proportion: Fangji 857g, Astragalus 857g, Guizhi 857g, Poria cocos 1714.3g, Licorice 571.4g, the above five flavors, add water and decoct twice, the first time for 2 hours, the second time for 1 hour, filter, and the filtrate Concentrate to a clear paste with a relative density of 1.20-1.25 (40° C.), add an appropriate amount of maltodextrin, mix, dry, and granulate to make 1000 g to obtain the Fangji Fuling Decoction composition.

Embodiment 2

[0040] Example 2. Establishment of Fangji Fuling Decoction Fingerprint

[0041] Instruments and reagents

[0042] Chromatography instrument: American Agilent 1100 series (G1322A online vacuum degasser, G1311A quaternary gradient pump, G1313A autosampler, G1316A column thermostat, G1315B diode array detector);

[0043] Column: Agilent ZORBAX SB C 18 5μm.4.6mm×250mm;

[0044] Reagents: Methanol and acetonitrile are chromatographically pure, and other reagents are analytically pure

[0045] Reference substance: Glycyrrhizic acid was purchased from Chengdu Master Biotechnology Co., Ltd., batch number MUST-14081812, with a purity of 98.23%.

[0046] Drugs: Composition of Fangji Fuling Decoction, batch numbers: 160401, 160402, 160403.

[0047] (1) Chromatographic conditions

[0048] The chromatographic column adopts octadecylsilane bonded silica gel chromatographic column; the flow rate is 1.0ml / min; the column temperature is 30°C; the detection instrument adopts an ultraviolet ...

Embodiment 3

[0056] Example 3. Methodological Investigation of Fingerprint of Fangji Fuling Decoction

[0057] Including stability, repeatability, and precision verification, the specific experimental results are shown below.

[0058] ① Inspection of precision

[0059] Prepare 1 part of need testing solution according to the method of this embodiment, inject 6 times continuously, measure fingerprint, overlay sees attached figure 1 . Calculate the relative standard deviation of the retention time and peak area of ​​some chromatographic peaks, see Table 1.

[0060] Table 1 precision examines part of the chromatographic peak retention time and peak area relative standard deviation

[0061]

[0062] ② Stability inspection

[0063] Prepare 1 part of the test solution according to the method of this example, inject samples at 0h, 3h, 6h, 8h, 14h, 19h, and 24h respectively, and determine the fingerprints. The overlay is shown in the accompanying drawing. Calculate the relative standard de...

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Abstract

The invention discloses an establishing method of a tetrandra and poria decoction fingerprint atlas and the fingerprint atlas, wherein the method and the fingerprint atlas belong to the field of traditional Chinese medicine analysis. The method of the invention is characterized in that high-efficiency liquid chromatography is used for establishing the fingerprint atlas, wherein the chromatogram conditions are described as follows: a chromatographic column is an octadecylsilane chemically bonded silica charomatographic column; the flow speed is 0.8ml / min-1.2ml / min; the column temperature is 20-35 DEG C; a detecting instrument is an ultraviolet detector, and the detecting wavelength of the fingerprint atlas is 237nm; a theoretical stage number is not lower than 5000 when calculated by Glycyrrhizic acid peaks; reference object solution is Glycyrrhizic acid solution; acetonitrile is used as a mobile phase A; 0.2% phosphoric acid and 0.2% triethylamine solution is used as a mobile phase B;and gradient elution is performed according to a sequence which is shown in the description. The fingerprint atlas comprehensively reflects quality information of a tetrandra and poria decoction composition, thereby realizing a purpose of more comprehensively and effectively controlling quality of the tetrandra and poria decoction composition.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine analysis, and relates to a quality standard detection method for traditional Chinese medicine compound granules, in particular to a quality control method for the composition of Fangji Fuling Decoction. Background technique [0002] Traditional Chinese medicine decoction is the mainstream of clinical medicine, but because of decoction and inconvenient to carry, the quality of the decoction prepared by decoction often varies greatly depending on the person and the decoction utensils. It is popular among consumers because it is convenient, effective, and cheap. Using modern science and technology to develop classic famous prescriptions into traditional Chinese medicine preparations that are easy to carry and take is not only the inheritance of traditional Chinese medicine, but also a better promotion of classic famous prescriptions. Clinical application. The preparation process of granul...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 刘志刚张超林丽娜陈周全谭沛马鹏岗石子仪高云佳屠鹏飞
Owner CHINA RESOURCES SANJIU MEDICAL & PHARMA
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