Indomethacin quantum dot immunochromatographic detection card based on signal amplification system and detection method
A quantum and signal amplification technology of indomethacin, which is applied in biological testing, material inspection products, etc., can solve the problems of low detection signal strength and sensitivity, inability to uniformly prepare signal substances, insufficient stability, etc., and achieve active protection. , detection time is short, and the effect of increasing antibody stability
Pending Publication Date: 2018-08-31
SHAOGUAN COLLEGE
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Problems solved by technology
The "binary system immunocompetition method" has the following disadvantages: 1. It is necessary to label the signal substance for each detection antibody separately, so that the signal substance cannot be prepared uniformly; 2. The detection antibody is in the liquid phase and has insufficient stability; 3. The detection signal intensity and less sensitive
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[0029] see figure 1 , which is the indomethacin quantum dot immunochromatographic detection card based on the signal amplification system of this embodiment, including a PVC liner, and detection antigens that are sequentially adhered to the PVC liner and partially overlapped with each other to release Pad, quantum dot probe release pad, nitrocellulose membrane (NC film) and water-absorbing pad; The detection antigen release pad comprises the detection antigen formed by coupling of OVA and indomethacin; The quantum dot probe releases The pad contains quantum dot probes labeled with anti-OVA antibody; the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is immobilized with anti-indomethacin antibody, and the quality control line is immobilized with OVA antigen.
[0030] Specifically, the preparation method of the detection antigen release pad is as follows: a glass cellulose membrane with a thickness of 0.85 mm is used to f...
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The invention relates to an indomethacin quantum dot immunochromatographic detection card based on a signal amplification system and a detection method. The detection card comprises a liner plate, a detection antigen release pad, a quantum dot probe release pad, a chromatographic membrane and a water absorbent pad; the detection antigen release pad comprises a detection antigen coupled by ovalbumin and indomethacin; the quantum dot probe release pad comprises a quantum dot probe labeled with an anti-ovalbumin antibody; the chromatographic membrane is provided with a detection line and a quality control line, the detection line fixes an anti-indomethacin antibody, and the quality control line fixes an ovalbumin antigen. The detection antigen is dissolved from the release pad into a detection system to form a ternary system immune complex with a detection antibody and a labeled antibody so as to generate a fluorescence detection signal. Under the detection and reference functions of thequality control line, whether a sample contains the indomethacin is judged according to a fluorescence signal on the detection line, the signal intensity and stability of the detection can be increased, and the detection sensitivity and the quantitative precision are improved.
Description
technical field [0001] The present invention relates to the field of food and drug safety detection, in particular to a method of applying immunochemical detection technology to the detection of illegally added chemicals in health care products and Chinese patent medicines, especially an indomethacin quantum dot immunochromatographic detection card based on a signal amplification system and detection methods. Background technique [0002] The chemical name of indomethacin is 2-methyl-1-(4-chlorobenzoyl)-5-methoxy-1H-indole-3-acetic acid, which belongs to non-steroidal anti-inflammatory drugs and has anti-inflammatory , analgesic and antipyretic effects, used for rheumatoid arthritis, adhesive spondylitis, non-inflammatory joint pain, arthritis, non-articular rheumatism, pain caused by non-articular inflammation, various neuralgia, cancer pain , post-traumatic pain and fever caused by various inflammations, etc., but it has certain side effects. It is a prescription drug in ...
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CPCG01N33/68
Inventor郭杰标黄婉琼林秀玉朱哲吴蓝洁张鹏
OwnerSHAOGUAN COLLEGE