Nasopharynx cancer illness risk detection kit

A detection kit and disease risk technology, applied in the field of biotechnology detection, can solve the problems of lack of sensitivity and specificity, and achieve the effect of early prevention

Inactive Publication Date: 2018-09-07
GUANGDONG MEDICAL UNIV
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Problems solved by technology

Although these indicators alone or in combination provide useful information for the diagnosis of nasopharyngeal carcinoma, they lack sufficient sensitivity and specificity

Method used

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  • Nasopharynx cancer illness risk detection kit

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Experimental program
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Effect test

Embodiment

[0017] 1 Case collection and grouping

[0018] In this experiment, blood samples were collected from patients with nasopharyngeal carcinoma and healthy subjects. The blood was collected in anticoagulant tubes and stored at -80°C until DNA was extracted. According to the case-control study, 336 cases of nasopharyngeal carcinoma patients admitted to the Affiliated Hospital of Guangdong Medical University from September 2014 to October 2015 and 397 cases of nasopharyngeal carcinoma patients admitted to Guangdong Nanfang Hospital from September 2010 to December 2011 were selected as the case group , were diagnosed as nasopharyngeal carcinoma by histopathology. The control group of 895 cases was collected from September 2014 to October 2015 at the Affiliated Hospital of Guangdong Medical University, with blood collected from age- and sex-matched healthy individuals with routine physical examination. All subjects lived in Guangdong Province, China. All subjects participating in thi...

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Abstract

The invention provides a nasopharynx cancer illness risk detection kit which is used for detecting human ATF1rs11169571 locus T / C mutation. The kit comprises protease K, a buffer solution GB, a buffersolution GD, a rinsing solution PW, ultrapure water, an ATF1 upstream primer, an ATF1 downstream primer, Go Taq Greeen Master Mix 2x, RNase Free H2O, Hind III enzyme, buffer 10x, a TBE buffer solution, agarose, ethidium bromide, and bromophenol blue indicator. The ATF1 upstream primer is 5'-CGCACGATATCTAGTGACAGAGG-3', and the ATF1 downstream primer is 5'-TGCAAACCTGTAGGGTAAATGG-3'.

Description

technical field [0001] The invention relates to the field of biotechnology detection, in particular to a nasopharyngeal cancer risk detection kit and a method for testing human ATF1 rs11169571 single nucleotide polymorphism. Background technique [0002] Nasopharyngeal carcinoma (NPC) is a common malignant tumor in southern my country, and Guangdong is a high-incidence area. Nasopharyngeal carcinoma refers to the cancer of nasopharyngeal mucosal epithelium, most of which are poorly differentiated squamous cell carcinomas, with high malignancy and hidden location, especially in the pharyngeal recess and the top of the nasopharynx. The early symptoms are not obvious, so it is difficult to early stage It was found that the rate of misdiagnosis and mistreatment is high, up to 12.2%. Therefore, in the diagnosed nasopharyngeal carcinoma, its 5-year survival rate hovers around 50% to 60% for a long time. Early diagnosis of nasopharyngeal carcinoma for early treatment and improving...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 何志巍黄国良彭述堂曾柳燕许南松
Owner GUANGDONG MEDICAL UNIV
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