Whitening anti-aging composition containing extract of dendrobium stem cells and use thereof
An anti-aging and composition technology, which is applied in the field of whitening and anti-aging compositions containing stem cell extracts of dendrobium to achieve the effect of improving skin quality, improving skin elasticity and preventing facial aging
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[0035]The preparation method of the dendrobium stem cell extract of the present invention is as follows: 1) select high-quality dendrobium leaves, clean and disinfect, and cut into powder to make callus; 2) inoculate the callus on a solid medium for induction Cultivate, cut the regenerated part of the callus for subculture, transfer the cultured product to a liquid medium for expanded culture, and then transfer to a bioreactor for large-scale culture until a saturated dendrobium stem cell suspension is obtained; 3) Filter the dendrobium stem cell suspension obtained in 2) with a 1 μm filter, remove the culture medium, and harvest the retained cells; put the cells in ultra-low temperature freezing at -80°C for 2 hours, then remove and thaw for 30 minutes, and repeat the above operation 5 times, after the last thawing at room temperature, break the cells with an ultrasonic breaker, filter through a filter, collect the filtrate, and concentrate it in a rotary vacuum evaporator to ...
Embodiment 1
[0043] The whitening and anti-aging composition of this embodiment includes the following ingredients in parts by weight: 1.5 parts of Dendrobium stem cell extract, 15 parts of Cordyceps militaris extract, 10 parts of papaya extract, and 8 parts of Danshen flower / leaf / root extract.
[0044] The above-mentioned whitening and anti-aging composition is applied to a cream, and the cream includes the following ingredients in mass percentage: 5% of the whitening and anti-aging composition, 8% of jojoba oil, 3% of glyceryl stearate, and 3% of cetearyl alcohol , PEG-10 stearate 2%, sodium hyaluronate 0.15%, carbomer 0.5%, potassium sorbate 0.1%, and the balance is deionized water.
[0045] 1) Mix jojoba oil, glyceryl stearate, cetearyl alcohol, and PEG-100 stearate together and heat to 80°C for 20 minutes; 2) Sodium hyaluronate, carbomer, Mix deionized water and potassium sorbate together and heat to 80°C, keep warm for 20 minutes; 3) Add the mixture in step 2) to step 1), stir evenly...
Embodiment 2
[0047] The whitening and anti-aging composition of this embodiment includes the following ingredients in parts by weight: 1 part of Dendrobium stem cell extract, 15 parts of Cordyceps militaris extract, 5 parts of papaya extract, and 5 parts of Danshen flower / leaf / root extract.
[0048] The whitening and anti-aging composition of this embodiment is applied in a cream, wherein the mass percentage of the whitening and anti-aging composition is 6%, and the remaining ingredients and contents and the preparation method of the cream are the same as in Example 1.
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