Application of Thonningianin A to preparation of drug for preventing or treating alcoholic cardiomyopathy
An alcoholic cardiomyopathy and drug technology, applied in drug combinations, cardiovascular system diseases, pharmaceutical formulations, etc., can solve the problems of left ventricular myocardial cell loss, mitochondrial damage, calcium homeostasis imbalance, etc. The effect of reducing myocardial damage
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[0024] 1. Materials:
[0025] Experimental animals: 50 C57BL / 6 male mice were purchased from Shanghai Slack Experimental Animal Co., Ltd., company license number: SCXK (Shanghai) 2017-0005, and were kept in the Experimental Animal Center of Zhejiang University of Traditional Chinese Medicine in an SPF environment.
[0026] Reagent: Thonningianin A (TA); Lieber-DeCarli alcohol liquid model feed (product code TP-4030B, Nantong Trophy Feed Technology Co., Ltd.); Lieber-DeCarli control liquid feed (product code TP-4030BC, Nantong Trophy Feed Technology Co., Ltd.); choline, vitamins (Nantong Trofe Feed Technology Co., Ltd.); absolute ethanol (CAS-NO: 64-17-5, lichrosolv); creatine kinase isoenzyme (Lot: N28018610, CUSABIO) .
[0027] 2. Method:
[0028] After the C57BL / 6 mice were adapted to feeding in the SPF level animal laboratory, the 8-week-old C57BL / 6 mice were randomly divided into 5 groups, 10 in each group, respectively, the control liquid feed group (PF group), the alco...
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