Application of a kind of angelica protein in the preparation of liver injury protection medicine

A liver injury prevention drug technology, applied in the application field of angelica protein in the preparation of liver injury protection drugs, to achieve the effect of improving liver cell necrosis, reducing damage, and reducing liver MDA levels

Active Publication Date: 2020-11-10
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Despite the tremendous advances in modern medicine and the availability of various hepatoprotective compounds from some plants, there are no fully effective drugs that can provide complete protection of the organ or help the liver regenerate

Method used

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  • Application of a kind of angelica protein in the preparation of liver injury protection medicine
  • Application of a kind of angelica protein in the preparation of liver injury protection medicine
  • Application of a kind of angelica protein in the preparation of liver injury protection medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: the purification of Angelica ASPR protein

[0032] Soak the Angelica sinensis cut into small pieces in 10 times the volume of 0.05 mol / L Tris-HCl buffer solution (pH 8.0), let it stand overnight at 4°C for about 15 hours, filter the residue with 4 layers of gauze the next day, and filter the filtrate at 12,000 rpm, 4 Centrifuge at ℃ for 10 minutes, and the obtained supernatant is the crude extract of angelica protein; the crude protein solution is subjected to one-step precipitation with ammonium sulfate (0-80%), precipitated overnight and collects the precipitated protein, which is dissolved in 2 times the volume of 0.05 mol / L Tris- In HCl buffer solution (pH 8.0), the ammonium sulfate precipitated solution was loaded onto the Sephadex G-50 chromatography column fully equilibrated with the equilibrium solution (0.05 mol / L, pH 8.0 Tris-HCl buffer solution), and the The balance solution was eluted and the second eluted peak was collected, and the purity was...

Embodiment 2

[0035] Example 2: Proliferative effect of Angelica ASPR protein on L-02 cells

[0036] MTT method was used to detect the effect of Angelica ASPR protein on the survival rate of L-02 cells. Cell viability was calculated according to the following formula.

[0037] Cell survival rate (%)=(OD value of measurement group-OD value of blank group) / (OD value of control group-OD value of blank group)×100.

[0038] Experimental results:

[0039] Angelica ASPR protein can effectively promote the proliferation of L-02 cells, and has a concentration-dependent relationship, such as figure 2 As shown, when the concentration was 0.063-0.50 mg / mLL, the survival rate of L-02 cells increased with the increase of the concentration. At 0.5-0.625 mg / mLL, the survival rate of L-02 cells decreased with the increase of the concentration, and at the dose of 0.5 mg / mLL, Angelica ASPR protein could make the proliferation rate of L-02 reach 163.14%.

Embodiment 3

[0040]Embodiment 3: to CCl 4 Dose-finding for the prevention of induced acute liver injury

[0041] 1. Experimental animals and grouping

[0042] Male Kunming mice, purchased from Wu's animals, weighing 20 ± 2 g. Normal feeding for three days before the experiment. The mice were randomly divided into 5 groups, 5 mice in each group, each group and its treatment were as follows: normal group (CON) and liver injury group (CCl 4 ) were injected with 0.5 mL of normal saline for 3 consecutive days, and 2 hours after the last administration, the CON group was injected with 0.2 mL of olive oil, CCl 4 Group injected with 0.1% CCl 4 Solution 0.2 mL (20 mL / kg); Protein plus drug group [ASPR1 (0.5mg / mL) + CCl 4 , ASPR2 (1.25mg / mL) + CCl 4 , ASPR3 (2.0mg / mL + CCl 4 )] Continuous injection of 0.5 mL of low, medium and high protein solution for 3 days respectively, 2 hours after the last administration, and then injection of 0.1% CCl 4 Olive oil solution 0.2 mL (20 mg / kg).

[0043] ...

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Abstract

The invention relates to the field of natural Chinese herbal protein, in particular to application of angelica protein to preparation of a medicine for protecting against acute liver damage. The N-terminal sequence of the angelica protein is GIQKTEVEAPSTVSA. The angelica protein has a significant proliferative effect on normal liver cells L-02, and at the dosage of 0.5mg / mL, the proliferation rateof L-02 cells can reach 163.114%. The angelica protein is applied to preparation of the medicine for preventing the liver damage, can significantly improve the CAT, GST and T-AOC activity of the liver of a damaged mouse and significantly reduce the MDA level of the liver, significantly improves the liver cell necrosis of the liver of the damaged mouse, thereby significantly reduces the blood ALTand AST activity and liver indexes of the damaged mouse to the normal group levels basically, and thus significantly reduces damage of CCl4 to the mouse liver.

Description

technical field [0001] The invention relates to the field of natural traditional Chinese medicine protein, and more specifically relates to the application of an angelica protein in the preparation of a medicine for protecting liver damage. Background technique [0002] The liver is one of the most important organs in the human body, responsible for the metabolism, secretion, storage and detoxification of endogenous and exogenous substances. Currently, liver disease remains one of the major threats to public health worldwide. Chemical liver injury refers to the liver injury caused by chemical hepatotoxic substances including alcohol, chemical toxic substances in the environment and certain drugs (such as chemotherapy drugs, etc.). If it is not treated in time, it will cause irreversible damage to the liver. Severe cases will lead to liver fibrosis, cirrhosis and even liver cancer. Despite the tremendous advances in modern medicine and the discovery of a variety of hepatopr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08A61K38/10A61P1/16
CPCA61K38/00A61P1/16C07K7/08
Inventor 潘剑茹王香玲
Owner FUZHOU UNIV
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