Unlock instant, AI-driven research and patent intelligence for your innovation.

New application of guava leaves and total flavonoids of guava leaves

A technology of guava leaves and total flavonoids, which is applied in the field of medicine and can solve the problems of no relevant literature and patents on the prevention and treatment of pancreatitis

Active Publication Date: 2021-08-27
天津市医药科学研究所
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing technology is mainly aimed at the hypoglycemic effect of total flavonoids of guava leaves, and some literatures have reported its antitumor activity, but there are no relevant literatures and patents for the prevention and treatment of pancreatitis.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • New application of guava leaves and total flavonoids of guava leaves
  • New application of guava leaves and total flavonoids of guava leaves
  • New application of guava leaves and total flavonoids of guava leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Extraction and content determination of guava leaf total flavonoids

[0017] Ethanol reflux extraction-recovery reagent under reduced pressure-concentration-filtration-petroleum ether treatment-ethyl acetate extraction and other steps for extraction. Determination of TFPGL content Use rutin as a standard substance to measure the absorbance value, take the concentration as the abscissa, and the absorbance as the ordinate, and draw a standard curve. Measure the absorbance at 500nm of the tested sample by ultraviolet spectrophotometry (rutin standard has a strong absorbance at 500nm, which is set as the measurement wavelength), and substitute it into the standard curve regression equation to calculate the total flavonoid content as 19.8%.

Embodiment 2

[0018] The acute toxicity of embodiment 2 guava leaf total flavonoids

[0019] 1. Experimental method:

[0020] Experimental Design Administration group and blank control group, 24 animals in each group, half male and half male. The administration dose of the administration group was 22.32g / kg, and the administration volume was 40ml / kg for intragastric administration. The control group was given equal volume of drinking water by intragastric administration.

[0021] The appearance, mental state, respiration, skin coat, feces and urine, eyes, ears, nose, mouth, genitals and other general conditions of the mice were observed and recorded daily, as well as other poisoning manifestations and death conditions. The body weight and food intake of the mice were measured once a week for 14 consecutive days.

[0022] 2. Experimental results:

[0023] General condition and death observation: After administration until the 14th day, all animals were in good mental and behavioral condi...

Embodiment 3

[0025] Example 3 Multi-index response surface method to optimize the assisted extraction process of total flavonoids from guava leaves

[0026] 1. Chromatographic conditions and methods:

[0027] The chromatographic column is Agilent Eclipse XDB-C18 (4.6mm×250mm, 5μm); A (methanol %), B (0.2% phosphoric acid aqueous solution %); gradient elution, 0~20.00min, 37%A; 20.01~35.00min, 70% A; volume flow rate: 1mL / min; column temperature: 35°C; detection wavelength: 360nm. Precisely draw 10 μL of the test solution, and determine hyperoside, quercetin-3-O-β-D-arabinopyranoside, quercetin-3-O-α-L-furan arabinoside in the test solution Glycoside peak area, calculate component content, combine these 3 component chromatographic peak areas to account for the percentage example of total peak area, convert the content of guava leaf total flavonoids in the test solution.

[0028] Total flavonoid content = total flavonoid content (mg / g) = sum of mass fractions of 3 kinds of flavonoid glycos...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the use of total flavonoids of guava leaves for preparing medicines for preventing and treating pancreatitis. The present invention further provides a pharmaceutical composition for preventing and treating pancreatitis, the pharmaceutical composition comprises an effective therapeutic dose of total flavonoids of guava leaves as an active ingredient, and a pharmaceutically acceptable carrier. The present invention shows through animal experiments that the total flavonoids of guava leaves can inhibit the excessive proliferation of pancreatic stellate cells by inhibiting the activation of P2X7R-mediated NLRP3 inflammasome signaling pathway, relieve the degree of pancreatic fibrosis, and have a good effect on preventing and treating pancreatitis. There is no toxic and side effect, and it can be used to prepare medicines for preventing and treating pancreatitis.

Description

technical field [0001] The invention relates to the field of medicine, in particular, the invention relates to a traditional Chinese medicine for preventing and treating pancreatitis and its total flavonoid extract. Background technique [0002] Pancreatitis can be acute (new, short-term) or chronic (persistent, long-term). [0003] Acute pancreatitis (AP) is a common disease causing significant morbidity and mortality. Organ failure and systemic inflammatory response syndrome occur in the early stage of the disease, and infection of necrotic tissue occurs in the late stage. At present, the treatment and prognosis of acute pancreatitis are still challenging, with the characteristics of high mortality, high disability rate, and high cost. Acute pancreatitis usually begins immediately after damage to the pancreas begins. The episodes are usually very mild. Mild episodes last for a short time and usually resolve completely when the pancreas returns to its normal state. Repeat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/61A61P1/18
CPCA61K36/61A61P1/18
Inventor 张桂贤刘洪斌刘大卫赵秀梅
Owner 天津市医药科学研究所
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com