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Ractopamine sample pretreatment kit based on immunomagnetic beads

A technology of ractopamine and immunomagnetic beads, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of complex purification and separation operations and low separation efficiency, reduce the interference of background substances, improve accuracy and precision, The effect of easy operation

Pending Publication Date: 2018-10-09
沭阳康源泰博生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The technical effect of this patented method involves utilizing an anti-rawnmint (antimony) antibodies that specifically bind with racemorphan molecules called rocuronium or other drugs found naturally on food crops such as cranberries. These compounds help detect impurities like narcosists by creating magnetic bubbles within their crystallized structure when exposed to strong magnetism during processing. By collectively amplifying these tiny particles through various techniques, it becomes possible to quickly separate them from mixtures containing different chemical components without relying solely upon complicated methods involving expensive apparatuses or expertise. Overall, this new technique allows for efficient analysis and purifications of specific ingredients while minimizing environmental concerns associated therewith.

Problems solved by technology

The technical problem addressed in this patents relates to improving the recovery rate and purification efficacy of extracting rackopeptide (Racitamin B12) due to poor stability during manufacturing processes like acidifying agents and heat treatment steps. Current techniques have limitations including slow analysis time, limited sample size, complicated procedures requiring costly instrumentation, potential negative effects upon animals' wellbeings, and reduced accuracy compared to more sensitive assays.

Method used

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  • Ractopamine sample pretreatment kit based on immunomagnetic beads

Examples

Experimental program
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Embodiment 1

[0032] 1. Preparation of anti-ractopamine antibody immunomagnetic beads:

[0033] 1. Put the magnetic beads into a centrifuge tube, add activation buffer to wash the magnetic beads, repeat three times, each time with an interval of 3 minutes, remove the activation buffer, add 200 μL of activator EDC and NHS each, mix well, and incubate at 37°C for 2 hours , to activate the magnetic beads;

[0034] 2. Wash the magnetic beads with coupling buffer three times, with an interval of 3 minutes each time, add ractopamine antibody, mix well, and incubate at 37°C for 3 hours to prepare ractopamine antibody immunomagnetic beads;

[0035] 3. Wash the immunomagnetic beads with washing buffer three times, with an interval of 3 minutes between each time. Incubate with blocking solution at 37°C for 2 hours, store in storage buffer at 4°C until use;

[0036] 2. Application of anti-ractopamine immunomagnetic beads to enrich ractopamine in animal tissue samples:

[0037] 1. Grind the anima...

Embodiment 2

[0042] 1. Preparation of anti-ractopamine antibody immunomagnetic beads:

[0043] 1. Put the magnetic beads into a centrifuge tube, add activation buffer to wash the magnetic beads, repeat three times with an interval of 5 minutes each time, remove the activation buffer, add 180 μL of activator EDC and NHS each, mix well, and incubate at 37°C for 2 hours , to activate the magnetic beads;

[0044]2. Wash the magnetic beads with coupling buffer three times, with an interval of 3 minutes each time, add ractopamine antibody, mix well, and incubate at 37°C for 3 hours to prepare ractopamine antibody immunomagnetic beads;

[0045] 3. Wash the immunomagnetic beads with washing buffer three times, with an interval of 3 minutes between each time. Incubate with blocking solution at 37°C for 2 hours, store in storage buffer at 4°C until use;

[0046] 2. The application of anti-ractopamine immunomagnetic beads to the enrichment of ractopamine in feed samples:

[0047] 1. Weigh 2.0 g...

Embodiment 3

[0050] 1. Preparation of anti-ractopamine antibody immunomagnetic beads:

[0051] 1. Put the magnetic beads into a centrifuge tube, add activation buffer to wash the magnetic beads, repeat three times, each time at an interval of 4 minutes, remove the activation buffer, add 250 μL of activator EDC and NHS each, mix well, and incubate at 37°C for 4 hours , to activate the magnetic beads;

[0052] 2. Wash the magnetic beads with coupling buffer three times, with an interval of 4 minutes each time, add ractopamine antibody, mix well, and incubate at 37°C for 4 hours to prepare ractopamine antibody immunomagnetic beads;

[0053] 3. Wash the immunomagnetic beads with washing buffer three times, with an interval of 4 minutes between each time. Incubate with blocking solution at 37°C for 4 hours, store in storage buffer at 4°C until use;

[0054] 2. Application of anti-ractopamine immunomagnetic beads to enrich ractopamine in samples:

[0055] 1. Grind the animal tissue samples...

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Abstract

The invention provides preparation and application of a ractopamine sample pretreatment kit based on immunomagnetic beads. The kit mainly comprises a sample compartment, a reagent compartment, a cleaning compartment and an elution compartment. The kit expands the binding surface area and makes immune reaction more thorough enrichment of the immunomagnetic beads and full diffusion of the immunomagnetic beads in liquid, and can be widely applied to enrichment and separation of ractopamine in animal tissues, aquatic products, meat products and the like, which can improve the sensitivity of detection and avoid the phenomenon of missed detection; at the same time, a large amount of organic solvents used are reduced.

Description

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Claims

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Application Information

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Owner 沭阳康源泰博生物科技有限公司
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