Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

VEGF (vascular endothelial growth factor)-C monoclonal antibody and kit

A VEGF-C, monoclonal antibody technology, applied in the direction of anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, biological testing, anti-animal/human immunoglobulin, etc., to achieve a simple and good detection method Biologically active, high-affinity effect

Inactive Publication Date: 2018-10-12
浙江众意生物科技有限公司
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for better understanding on how vascular endothelial growth factor (VEG) works with certain proteins that are involved in blood vessel formation during embryogenesis or wound healing process. It can be used alone or combined with other agents like chemotherapy drugs to improve their effectiveness against these diseases.

Problems solved by technology

This patents describes how VEGFA works when combined with another substance called VEGFC proteins found within these two types of membranes - VEGFL1/vf1-R_EVH-1+vetrointestinal tract vasculature system and Laminarger's complex network involved in nerve fibers transporting signals from peripheral organs like brain stem and spindle germens. By measuring levels of both VEGFOCS and EML-4, we may predict whether patients develop lungmetastasis early during therapy or later at advanced stages. Additionally, our findings suggest that increasing intravenously administered drugs could improve survival rates of breast cancer and reduce recurrence rate.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • VEGF (vascular endothelial growth factor)-C monoclonal antibody and kit
  • VEGF (vascular endothelial growth factor)-C monoclonal antibody and kit
  • VEGF (vascular endothelial growth factor)-C monoclonal antibody and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Preparation of VEGF-C antigen:

[0023] (1) Design a pair of primers according to the sequence of human VEGF-C (X94216.1) in the GenBank database. The upstream primer is vP: 5'-ATACCCCGCCACGGACCGGTCCCCCACCCCCGGTCC-3', and the Xho I restriction site is introduced; the downstream primer is vP: 5 '-TTCATATTGGAAAGACCACAAATGAGCTA-3', introduce EcoR I restriction site, extract total RNA from human hepatocytes by RT-PCR method, then reverse transcribe into cDNA, use cDNA as a template, and use Pyrobest DNA polymerase for VEGF-C Gene-specific amplification. The amplified PCR product was recovered from the gel, and the recovered VEGF-C gene and pPIC9K plasmid were digested with Xho I and EcoR I respectively, and the recovered target fragment was ligated with T4 ligase, and the ligated product was transformed into DH5α competent cells, passed through ampicillin (1 mg / mL) antibody screened positive clones, and the plasmids were extracted and identified by enzyme digestion and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a VEGF-C monoclonal antibody. The amino acid sequence of a heavy chain of the monoclonal antibody is as shown as VH: CAA63907, and the amino acid sequence of a light chain of the monoclonal antibody is as shown as VL: 2X1W_D. The VEFG-C monoclonal antibody has the relatively high affinity, strong specificity and good biological activity in vitro. A VEGF-C protein detectionkit prepared from VEGF-C monoclonal antibody and VEGF-C antibodies can serve as indexes for assessing tumor lymphangion genesis and lymphatic metastasis.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Owner 浙江众意生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com