Method for simultaneously detecting five fat-soluble vitamins, and application thereof

A fat-soluble vitamin and tocopherol technology, applied in the field of analysis and detection, can solve the problems that the specificity and sensitivity of high performance liquid chromatography cannot meet the clinical detection requirements, cannot comprehensively and effectively evaluate the nutritional level of vitamin E, and limit the scope of application, etc. Achieve the effect of low cost, high sensitivity and short analysis time
CN108645945AInactive Publication Date: 2018-10-12北京爱普益医学检验中心有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
北京爱普益医学检验中心有限公司
Publication Date
2018-10-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for simultaneously detecting five fat-soluble vitamins, and an application thereof. The method comprises the following steps: (1) analyzing a pretreated sample by a liquid chromatograph-mass spectrometer; and (2) calculating the concentrations of the five fat-soluble vitamins in the sample to be tested according to a standard curve, wherein the fat-soluble vitaminscomprise retinol, alpha-tocopherol, beta-tocopherol, gamma-tocopherol and delta-tocopherol. A chromatographic column in the liquid chromatograph-mass spectrometer adopts gradient elution, and the mobile phase for the gradient elution is an ammonium formate-acetonitrile mixed solution. The recovery rate, the detection limit, the precision and other technical indicators of the method meet requirements, and isotope labels are used as internal standard substances, so the target compounds can be recognized in the manner of strong specificity, high sensitivity, good accuracy and short analysis time,and the method has a good reproducibility and a small error.
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Description

technical field

[0001] The invention belongs to the technical field of analysis and detection, and relates to a method and application for simultaneously detecting five kinds of fat-soluble vitamins. Background technique

[0002] Vitamin A and vitamin E are fat-soluble vitamins necessary for the human body, mainly from food, and the lack of vitamin A and vitamin E will affect the growth and development of the human body. Vitamin A is mainly retinol, which participates in the synthesis of rhodopsin, and has effects on the pleiotropic behavior of various physiological functions such as growth, reproduction, hematopoiesis and immunity of the human body (Albarhani A A, Collier F, Greaves R F, et al. D and A can be successfully measured by LC-MS / MS in cord blood diluted plasma.Clinical Biochemistry,2015,48:1105;Villamor E,Fawzi W W.Effects of vitamin A supplementation on immune responses and correlation with clinical outcomes.Clinical Microbiology Reviews ,2005,18:446.), lack of...

Claims

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