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An oligopeptide that promotes the proliferation and migration of intestinal mucosal epithelial cells and its preparation method and application

A technology for epithelial cells and intestinal mucosa, applied in the field of oligopeptides that promote the proliferation and migration of intestinal mucosal epithelial cells and their preparation

Active Publication Date: 2021-07-13
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The casein glycomacropeptide has also shown the effect of alleviating the symptoms of IBD in animal experiments, but it is still under research and improvement

Method used

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  • An oligopeptide that promotes the proliferation and migration of intestinal mucosal epithelial cells and its preparation method and application
  • An oligopeptide that promotes the proliferation and migration of intestinal mucosal epithelial cells and its preparation method and application
  • An oligopeptide that promotes the proliferation and migration of intestinal mucosal epithelial cells and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 (bromelain)

[0044] (1) Wash the shellfish meat (oysters are used in the examples of the present invention), take out the meat, grind it, and add pure water to homogenize according to the weight-to-volume ratio (kg / L) of 1:2. According to the proportion of 3000U / g shellfish meat, add bromelain, adjust the pH value to 6.5, and hydrolyze at 40°C for 4 hours. Heating to 95°C for 10 minutes to inactivate the enzyme, cooling, filtering and concentrating to obtain the enzymolysis concentrated liquid of shellfish meat. The concentrated shellfish meat supernatant was treated with 0.2 μm, 100,000 and 3,000 MWCO ultrafiltration membranes to remove large proteins, low molecular weight amino acids and small molecular compounds, and obtained ultrafiltration fractions with a nominal molecular weight of 100-3 kDa, which were concentrated and freeze-dried to obtain shellfish Meat enzymatic hydrolysis ultrafiltration fraction dry powder A.

[0045] (2) The ultrafiltration d...

Embodiment 2

[0047] Embodiment 2 (papain)

[0048] (1) Wash the shellfish, take the meat, crush it, and add pure water to homogenize it according to the weight-to-volume ratio (kg / L) of 1:3. According to the proportion of 4000U / g shellfish meat, add papain, adjust the pH value to 6.5, and hydrolyze at 50°C for 4 hours. Heating to 95°C for 10 minutes to inactivate the enzyme, cooling, filtering and concentrating to obtain the enzymolysis concentrated liquid of shellfish meat. The concentrated shellfish meat serum is treated with 0.2μm, 100,000 and 5,000MWCO ultrafiltration membranes to remove large proteins, low molecular weight amino acids and small molecular compounds, and obtain ultrafiltration fractions with a nominal molecular weight of 100~5kDa, which are concentrated and freeze-dried to obtain shellfish meat Enzymatic hydrolysis ultrafiltration component dry powder A.

[0049] (2) The ultrafiltration dry powder A was dissolved into a 50mg / ml solution, separated and purified by Seph...

Embodiment 3

[0051] Example 3 (pepsin)

[0052] (1) Wash the shellfish, take the meat, crush it, and add pure water to homogenize it according to the weight-to-volume ratio (kg / L) of 1:4. According to the proportion of 1000U / g shellfish meat, add pepsin, adjust the pH value to 3.0, and hydrolyze at 40°C for 4 hours. Heating to 95°C for 10 minutes to inactivate the enzyme, cooling, filtering and concentrating to obtain the enzymolysis concentrated liquid of shellfish meat. The concentrated shellfish meat supernatant was treated with 0.2 μm, 100,000 and 10,000 MWCO ultrafiltration membranes to remove large proteins, low molecular weight amino acids and small molecular compounds, and obtained ultrafiltration fractions with a nominal molecular weight of 100-10 kDa, which were concentrated and freeze-dried to obtain shellfish Meat enzymatic hydrolysis ultrafiltration fraction dry powder A.

[0053] (2) Ultrafiltration dry powder A was dissolved into a 50 mg / ml solution, separated and purified...

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Abstract

The invention belongs to the field of functional food biotechnology and discloses an oligopeptide that promotes the proliferation and migration of intestinal mucosal epithelial cells. The amino acid sequence of the oligopeptide is: Val-Ala-Pro-Glu-Glu-His-Pro-Val One or both of ‑Leu‑Leu or Ser‑Tyr‑Glu‑Leu‑Pro‑Asp‑Gly‑Gln‑Val‑Ile‑Thr‑Ile‑Gly‑Asn‑Glu‑Arg. The peptide components and oligopeptides prepared by the present invention can significantly promote the migration of intestinal mucosal epithelial cells; and have selective effect on the proliferation of intestinal mucosal cells, and the promotion effect on normal cultured cells is not obvious, but under the culture conditions in which bacterial lipopolysaccharide exists , showing an obvious promoting effect. The oligopeptide of the present invention is obtained from oysters, has abundant sources, and adopts bio-enzyme hydrolysis technology in the preparation process, is safe and reliable, and has good application prospects in the fields of food and medicine.

Description

technical field [0001] The invention belongs to the field of functional food biotechnology, and more specifically relates to an oligopeptide for promoting the proliferation and migration of intestinal mucosal epithelial cells and its preparation method and application. Background technique [0002] Inflammatory bowel disease (Inflammatory bowel disease, IBD) is a common disease in developed countries and regions such as North America and Europe, but its incidence has been increasing in countries and regions such as Asia, Australia and Africa in recent years. With the rapid development of industrialization and urbanization in my country in recent decades, the incidence of IBD in China has been increasing year by year. The onset of IBD will not only seriously affect the normal life of patients, but also have a high risk of canceration, which has attracted widespread attention in the medical field. The therapeutic drugs for IBD mainly include chemical drugs, immunosuppressive ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K7/08C07K1/36C07K1/34C07K1/20C12P21/06C12N5/071A23L33/18A61K38/08A61K38/10A61P1/04
CPCA23V2002/00A61K38/00A23L33/18A61P1/04C07K7/06C07K7/08C12N5/0625C12N5/0679C12P21/06A23V2200/32A23V2250/55A23V2250/156A23V2250/54A23V2250/18A23V2250/70
Inventor 潘剑宇蔡冰娜陈华万鹏陈得科孙恢礼孙大儒
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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