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Plant probiotic flora constructed by beneficial bacterium mutant strain and application of plant probiotic flora

A technology of plant probiotics and mutant strains, which can be used in plant growth regulators, plant growth regulators, applications, etc., and can solve problems such as the stability of the flora

Active Publication Date: 2020-10-27
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the mutant strains in the prior art are prone to trade-off trade-off effects, and the construction of multiple strains faces the stability of the bacterial population.

Method used

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  • Plant probiotic flora constructed by beneficial bacterium mutant strain and application of plant probiotic flora
  • Plant probiotic flora constructed by beneficial bacterium mutant strain and application of plant probiotic flora
  • Plant probiotic flora constructed by beneficial bacterium mutant strain and application of plant probiotic flora

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Experimental program
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Effect test

Embodiment 1

[0043] The acquisition of embodiment 1 bacterial classification

[0044] 1.1 Bacillus amyloliquefaciens SQR9 wild strain

[0045] The wild strain of Bacillus amyloliquefaciens SQR9 was screened by the Laboratory of Plant Nutrition Research Group, School of Resources and Environmental Sciences, Nanjing Agricultural University. It was stored in the General Microbiology Center of China Committee for the Collection of Microbial Cultures. The preservation date is February 27, 2012, and the preservation number is It is CGMCC NO.5808, see Chinese patent 201310466680.1 for details.

[0046] 1.2 Mutant strain of Bacillus amyloliquefaciens SQR9

[0047] Based on PCR and reverse breeding marker pheS* multi-gene seamless knockout technology in Bacillus, through two rounds of homologous recombination to achieve targeted knockout of target genes and deletion of resistant fragments, the following mutant strains of Bacillus amyloliquefaciens SQR9 were obtained: 1) m3 scarless mutant: enhanc...

Embodiment 2

[0054] Embodiment 2 performance comparison of probiotic group

[0055] 2.1 Combination co-cultivation of strain SQR9 and its mutants

[0056] The strain SQR9 wild type and its six mutants with different functions (m3, m4 gene scarless mutants and m5, m1, m6, m2 scarless overexpression strains) were divided into 1 strain, 3 strains, 5 strains and 7 strains (Table 1) Combinations (a total of 64 groups) were carried out for liquid co-cultivation. The medium used was LB liquid medium, the culture condition was 180 r / m, 37 ° C for 10 h, and 3 replicates were designed for each group. The inoculum amount of the first generation is 1% (OD600=1), and the inoculum amount of the second generation is 1% of the bacterial liquid of the previous generation. The growth curve of the strain SQR9 shows that the strain enters the logarithmic growth phase after being cultivated for 2 hours, and the cell density basically reaches a stable period after being cultivated for 10 hours. Therefore, in t...

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Abstract

The invention provides a plant probiotic flora constructed by a beneficial bacterium mutant strain and application of the plant probiotic flora. The probiotic flora comprises a strain SQR9 mutant strain m4 and at least one strain SQR9 wild type or other mutant strains of the strain SQR9. The probiotic flora provided by the invention adopts probiotic flora screened by long-term co-evolution, has extremely high stability, and has a remarkable growth promoting effect on crops; and growth of plants can be obviously promoted.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to a plant probiotic group constructed from a mutant strain of beneficial bacteria and an application thereof. Background technique [0002] Bacillus amyloliquefaciens is a kind of plant beneficial bacteria widely distributed in nature, environment-friendly, and strong stress resistance. It produces good growth-promoting and The biocontrol effect has become an important development object of biological agents and biofertilizers. [0003] In the past, domestic and foreign research mainly focused on the influence of single bacteria on crop growth, and now more and more studies have shown that rhizosphere probiotics are of great significance to crop growth. Combined with modern molecular biology techniques, the mechanism of probiotics in the rhizosphere to promote growth and antagonize pathogenic microorganisms at the single-bacteria level has been preliminarily clarified. Taking antagonis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08A01N63/22A01P21/00C12R1/07
CPCA01N63/22C05F11/08C12N1/205C12R2001/07
Inventor 沈其荣刘红军李荣孟令雪李洪旭徐志辉张楠
Owner NANJING AGRICULTURAL UNIVERSITY
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