A multimodal nanoprobe for guiding precise resection of glioma
A nano-probe and multi-modal technology, applied in preparations for in vivo experiments, general/multifunctional contrast agents, drug delivery, etc., to achieve weak autofluorescence, high sensitivity, and simple operation
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Embodiment 1
[0030] Embodiment 1, the synthesis of targeted nanoprobe and control probe
[0031] Synthetic route such as figure 1 As indicated, polyethylene glycol SH-PEG-NHS (compound 1, 40mg, 0.02mmol) and polypeptide FAL (12.12mg, 0.02mmol) were dissolved in 0.3mL DMF, stirred at room temperature for 2h to obtain PEG derivative 2. 500mL 0.294mM tetrachloroauric acid solution, boiled, quickly added 5mL of 38.8mM sodium citrate aqueous solution, reflux reaction for 5min, the reaction product was purified by a dialysis bag with a molecular weight cut-off of 10000 to obtain citric acid-modified gold nanoparticles 3, particle size About 60nm. Diethylenetriaminepentaacetic dianhydride (10mg, 28μmol) and 2-mercaptoethylamine (1mg, 28μmol) were dissolved in 0.5mL DMF, stirred at room temperature for 4h to obtain compound 4. Compound 4 was added dropwise into 10 mL of gold nanoparticles 3 (0.13 μM), and reacted with stirring at room temperature overnight to obtain gold nanoparticles 5. GdCl ...
Embodiment 2
[0032] Embodiment 2, the transmission electron microscope picture of nanoprobe
[0033] Take an appropriate amount of nano-probes, drop them on the special copper grid for transmission electron microscopy, place them in a 40-degree oven for drying, and collect TEM images through transmission electron microscopy, as shown in Figure 2 shows The results show that the particle size of the nanoprobe is about 60nm, the dispersion is uniform, and the gold core is wrapped with a translucent polyethylene glycol layer.
Embodiment 3
[0034] Embodiment 3, to the mensuration of the hydrodynamic particle size distribution of nanoprobe and Zeta potential
[0035] Determination of the hydrodynamic radii of the target nanoprobe AuP-FAL and the reference probe AuP-PEG by dynamic light scattering at room temperature; equipment calibration was performed with a 2.0 mg / ml bovine serum albumin standard solution in distilled water , the sample was filtered with a 0.45μm filter head and diluted to 100g / mL with 1X PBS of pH 7.4, and the hydrodynamic radius and size distribution were calculated by a regularized formula; when the surface charge of the nanoprobe was determined, a Zeta Calibrate the equipment with a standard solution with a potential of -50mV, filter the nanoprobe drug solution with a 0.45μm filter head and dilute it to 200g / mL with 10mM NaCl solution;
[0036] Figure 3 shows It is shown that the average diameter of AuP-FAL is 61.2nm and 60.7nm, and the average zeta potential is -23.3mV.
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