Wide-field tomographic hyperspectral microscopic imaging method and device based on spatio-temporal focusing

A microscopic imaging and hyperspectral technology, which is applied in the field of microspectral imaging and analytical chemistry, can solve the problems of signal crosstalk, unsuitable scattering tissue spectral imaging, and axial resolution reduction, and achieve low scattering signal crosstalk and low high-speed spectroscopy. Effects of Information Acquisition, High Temporal Resolution

Active Publication Date: 2020-04-24
TSINGHUA UNIV
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  • Claims
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Problems solved by technology

On the other hand, although nonlinear optical microscopy using surface excitation avoids the speed bottleneck caused by point-by-point scanning, the excited signal has serious crosstalk after being scattered by tissue, which is not suitable for scattering tissue spectral imaging.
Ordinary nonlinear optical microscopy using line-scanning method can compromise between imaging speed and suppression of scattering effects, but this method is not an ideal choice because of the lower axial resolution obtained by point-scanning method

Method used

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  • Wide-field tomographic hyperspectral microscopic imaging method and device based on spatio-temporal focusing
  • Wide-field tomographic hyperspectral microscopic imaging method and device based on spatio-temporal focusing
  • Wide-field tomographic hyperspectral microscopic imaging method and device based on spatio-temporal focusing

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Embodiment 1

[0049] Refer below image 3 , describe in detail the wide field of view tomographic hyperspectral microscopic imaging device of this embodiment, the device includes an ultrashort pulse laser source and a beam conversion system, a line scanning system based on spatiotemporal focusing, an optical microscopic system, filtering and synchronous spectral sharing focus detection system, the biological sample is placed on the sample stage 319. Wherein, the ultrashort pulse laser source and the ultrashort pulse laser source 301 in the beam conversion system adopt femtosecond lasers (such as Coherent Chameleon Discovery series), and the beam conversion system adopts a Kepler telescope system composed of a lens 302 and a cylindrical lens 303 ( is 4f system); line scanning system based on spatio-temporal focusing includes transmission grating 304, lens 305 and scanning galvanometer 307; optical microscope system includes two lenses 308, 309 and microscope objective lens 310; filtering and...

Embodiment 2

[0052] Refer below Figure 4 , the wide-field tomographic hyperspectral microscopic imaging device of this embodiment is described in detail. The difference between this embodiment and Embodiment 1 is that an adaptive optical element is added. The device in this embodiment includes an ultrashort pulse laser light source and a beam conversion system, a line scanning system based on spatiotemporal focusing, an optical microscope system, a filtering and synchronous spectral confocal detection system, and biological samples are placed on the sample stage 424; wherein, the ultrashort The ultrashort pulse laser source 401 in the pulsed laser light source and the beam conversion system uses a femtosecond laser (such as Coherent Chameleon Discovery series), and the beam conversion system uses a Kepler telescope system composed of a lens 402 and a cylindrical lens 403 (a 4f system) The line scanning system based on spatio-temporal focusing includes a transmission grating 404, five lens...

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Abstract

The present invention relates to the technical field of microscopic spectral imaging and analytical chemistry, and disclosed thereby are a spatial and temporal focusing-based wide field of view chromatography hyperspectral microscopic imaging method and device. The method comprises: utilizing an ultrashort pulse laser source to produce an ultrashort pulse laser; producing a focal line in a sample by means of using spatial and temporal focusing; collecting an excited fluorescent light and filtering out stray light by using a confocal optical slit; collecting fluorescence spectrum information to complete the acquisition of spectrum information (x, λ) of the sample; and finally, acquiring five-dimensional information (x, λ, y, z, t) of the sample by means of three-dimensional spatial scanning and delayed scanning. The device comprises an ultrashort pulse laser source and beam conversion system, a spatial and temporal focusing-based line scanning system, an optical microscopic system, and a confocal detection system which filters and synchronizes spectrums; the acquisition of spectrum information in the confocal detection system which filters and synchronizes spectrums is synchronized with a line scanning trigger signal in the line scanning system which combines spatial and temporal focusing technology. The present invention has the advantages of a wide field of view, high spatial resolution, high temporal resolution, high spectral resolution and the like.

Description

technical field [0001] The invention relates to a wide field of view tomographic hyperspectral microscopic imaging method and device based on spatiotemporal focusing, and belongs to the technical fields of microspectral imaging and analytical chemistry. Background technique [0002] Hyperspectral Microscopy (Hyperspectral Microscopy) has important applications in the field of biomedical research, especially in the fields of clinical disease diagnosis, intraoperative image navigation and other fields, which have attracted more and more attention. Using hyperspectral microscopic imaging technology to obtain spatially resolved spectral information can provide information on physiological parameters, morphology and components of biological tissues for disease diagnosis. At present, non-invasive detection of various cancers can be realized by using hyperspectral microscopic imaging technology. [0003] In essence, hyperspectral microscopic imaging technology is a technology to o...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): A61B5/00
CPCA61B5/0073
Inventor孔令杰谢浩张元龙戴琼海
OwnerTSINGHUA UNIV