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Injectable composite gel and preparation method and application thereof

A technology of composite gel and mixed gel, which is applied in the fields of medical formula, medical science, prosthesis, etc., can solve the problems of large damage to the body, easily infected materials, and inability to adapt to the damaged part well, so as to reduce the inflammatory response and broaden the Application prospects, the effect of promoting migration and aggregation

Inactive Publication Date: 2018-11-06
SOUTHWEST JIAOTONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Another object of the present invention is to provide an application of an injectable composite gel, which can solve the shortcomings of the implanted material in tissue engineering, such as large damage to the body, easy infection, and the material cannot be well adapted to the damaged site, and it can promote heart health. Angiogenesis and Necrotic Tissue Repair

Method used

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  • Injectable composite gel and preparation method and application thereof
  • Injectable composite gel and preparation method and application thereof
  • Injectable composite gel and preparation method and application thereof

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preparation example Construction

[0025] For the preparation method of an injectable composite gel provided by the embodiment of the present invention, please refer to figure 1 , which includes:

[0026] S1. Prepare a chitosan solution with a mass fraction of 2%-2.5%, a gelatin solution with a mass fraction of 0.5%-2%, and a β-sodium glycerophosphate solution with a mass fraction of 50%-60%.

[0027] Further, the reason why the embodiments of the present invention limit the mass fractions of chitosan solution, gelatin solution and sodium β-glycerophosphate solution is to ensure the structure and performance characteristics of the subsequently prepared injectable composite gel.

[0028] Further, the embodiment of the present invention chooses chitosan as the raw material for preparing injectable composite gel because chitosan is the only natural cationic polysaccharide found so far, which has good biocompatibility, biodegradability, Antibacterial and other properties.

[0029] S2. Sterilize the chitosan solut...

Embodiment 1

[0044] This embodiment provides a method for preparing an injectable gel, which includes:

[0045] First, chitosan was dissolved in 0.2% glacial acetic acid solution to obtain a chitosan solution with a mass fraction of 2.2%; gelatin was dissolved in deionized water to obtain a gelatin solution with a mass fraction of 1%; The solution was dissolved in deionized water to obtain a 56% sodium β-glycerophosphate solution.

[0046] The chitosan solution and gelatin solution obtained above were sterilized under high temperature and high pressure at 121° C. for 15 minutes, and the β-sodium glycerophosphate solution was filtered with a 0.22 μm filter.

[0047] Further, after mixing the chitosan solution and gelatin solution in a ratio of 10:1, slowly add β-sodium glycerophosphate solution dropwise under ice bath conditions according to the volume ratio of chitosan solution and sodium β-glycerophosphate solution at a volume ratio of 5:1. , to obtain chitosan-based hydrogel (ie, the fi...

Embodiment 2

[0051] This embodiment provides a method for preparing an injectable gel, which includes:

[0052] First, chitosan was dissolved in 0.2% glacial acetic acid solution to obtain a chitosan solution with a mass fraction of 2.2%; gelatin was dissolved in deionized water to obtain a gelatin solution with a mass fraction of 1%; The solution was dissolved in deionized water to obtain a 56% sodium β-glycerophosphate solution.

[0053] The chitosan solution and gelatin solution obtained above were sterilized under high temperature and high pressure at 121° C. for 15 minutes, and the β-sodium glycerophosphate solution was filtered with a 0.22 μm filter.

[0054] Further, after mixing the chitosan solution and gelatin solution in a ratio of 10:1, slowly add β-sodium glycerophosphate solution dropwise under ice bath conditions according to the volume ratio of chitosan solution and sodium β-glycerophosphate solution at a volume ratio of 7:1. , to obtain chitosan-based hydrogel (ie, the fi...

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Abstract

An injectable composite gel and a preparation method and an application thereof mainly relate to the field of tissue engineering. The preparation method comprises mixing a chitosan solution, a gelatinsolution and a sodium Beta-glycerophosphate solution according to the certain ratio and sequence and adding a certain amount of growth factors and seed cells to make the prepared gel have the performance of repairing multiple kinds of tissues. The injectable composite gel prepared by the method not only has a certain factor sustained release effect but also can compound various cells to provide cell support for tissue repair, thereby better adapting to the injury part, reducing inflammation, promoting migration and aggregation of the cells and differentiation of the seed cells and promoting the formation of new blood vessels and the repair of necrotic tissues. Therefore, the injectable composite gel, the preparation method and the application have broad application prospects.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to an injectable composite gel and its preparation method and application. Background technique [0002] In the process of tissue engineering vascularization, scaffold materials, growth factors and seed cells are indispensable components. Among them, scaffold materials can not only provide cells with a suitable three-dimensional environment for survival, but also promote tissue engineering. of vascularization. As the most basic cell in the composition of blood vessels, EC is an important seed cell in the study of vascularization in tissue engineering. Although there have been many studies based on vascularization of endothelial cells, the acquisition of endothelial cells must be done at the expense of autologous blood vessels. The damage will also increase when the amount of cells obtained is large; however, MSCs are widely sourced and easy to obtain, and MSCs can be induced to di...

Claims

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Application Information

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IPC IPC(8): A61L27/50A61L27/52A61L27/20A61L27/22A61L27/12A61L27/00A61L27/38A61L27/54
CPCA61L27/00A61L27/12A61L27/20A61L27/222A61L27/3834A61L27/3882A61L27/50A61L27/507A61L27/52A61L27/54A61L2300/414A61L2300/602A61L2400/06A61L2430/22C08L5/08
Inventor 赵安莎王亚男苏红贺丹杨苹黄楠
Owner SOUTHWEST JIAOTONG UNIV
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