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Preparation method and application of coreopsis tinctoria extract containing maritimetin

A technology of extract and snow chrysanthemum, which is applied in the direction of food ingredients, applications, and food extraction containing natural extracts, to achieve the effects of stable efficacy, small dosage of drugs, and high purity

Active Publication Date: 2018-11-13
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The inventor’s previous studies have shown that the total flavonoids of Snow Chrysanthemum and its components such as mariside and nomarin have good lipid-lowering effects [Gao Fei, Research on the blood lipid-lowering effect and mechanism of total flavonoids of Snow Chrysanthemum, Master’s Degree Thesis of Soochow University, 2016], but whether snow chrysanthemum has the effect of regulating intestinal flora, and whether there are other unknown active ingredients in the body, need further in-depth study

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  • Preparation method and application of coreopsis tinctoria extract containing maritimetin

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preparation example Construction

[0024] In the preparation process of the snow chrysanthemum extract containing coniferous glucoside, the solution of the snow chrysanthemum extract can be selected from water and a solution containing an organic solvent, the pH range is preferably pH 6-12, and the temperature range is preferably 35-80° C. The reaction time range is preferably 0.5-10 h, and the purpose is to promote the dissolution of the chrysanthemum extract and the conversion of mariside. Wherein, the reagents for adjusting pH can be optional conventional acids and bases, preferably inorganic substances, such as sodium hydroxide, potassium hydroxide, sodium carbonate, sodium bicarbonate, hydrochloric acid, sulfuric acid, acetic acid and the like.

[0025] In the process of researching the preparation process of the snow chrysanthemum extract, the present invention unexpectedly found that the mariside (1) in the snow chrysanthemum extract would be degraded under neutral and weak alkaline heat preservation and ...

Embodiment 1

[0030] Example 1: Preparation of Snow Chrysanthemum Extract Containing Intriniflorin (I)

[0031] Dried snow chrysanthemum (Coreopsis tinctoria, place of origin Dabancheng, Xinjiang, batch 130501) 100g, reflux extraction with 2L of 75% ethanol twice, each time for 1h, combine the two extracts, filter, concentrate under reduced pressure to recover ethanol, add appropriate amount Diluted to 300mL with water, refined with AB-8 resin column, eluted with 6BV of water and 4BV of 75% ethanol successively after loading the sample, collected the eluted parts of 75% ethanol, concentrated, dried, and obtained the initial extract of chrysanthemum (CTE, 12.2g , batch 20160720). Using C18 reversed-phase silica gel column chromatography, mariside (1) and flavonomaside (3) were separated from CTE.

[0032] Weigh 25mg of CTE, add ethanol to 5ml; respectively take 0.4ml into EP tubes, add 1.6ml of pH6.8 phosphate buffer saline or artificial intestinal juice (prepared according to the 2015 Chin...

Embodiment 2

[0036] Example 2: Preparation of Snow Chrysanthemum Extract Containing Intriniacoside (II)

[0037] In this example, mariside obtained from the primary extract of Snow Chrysanthemum was used as a raw material to further study the conversion reaction product and its mechanism.

[0038] Weigh 25mg of mariside, add ethanol to make up to 5ml; take 0.4ml into EP tubes, add 1.6ml of pH6.8 phosphate buffer or artificial intestinal juice (prepared according to the 2015 Chinese Pharmacopoeia), vortex and mix well After incubation at 37±0.5°C for 24 hours, 0.1 ml samples were taken at the predetermined time points of 0, 2, 4, 6, 8 and 24 hours respectively, which was the mariside metabolite sample solution.

[0039] Result: see the test result figure 2 . Quantitative analysis by HPLC showed that in the sample solution of mariside metabolites, mariside (1) was quantitatively converted into seacoside (2), and the conversion was basically complete at 24 hours. In addition, 2-6 hours af...

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Abstract

The invention discloses a preparation method of a coreopsis tinctoria extract containing maritimetin and an application of the coreopsis tinctoria extract to preparation of a functional food capable of regulating intestinal flora function and metabolic diseases. The preparation method of the coreopsis tinctoria extract comprises the following steps of taking a coreopsis tinctoria extract solutioncontaining marein, regulating pH to be 5-14, performing heat preservation at 30-100 DEG C for definite time, regulating the pH to be 3-5, performing concentration, and performing drying so as to obtain the coreopsis tinctoria extract. The coreopsis tinctoria extract contains maritimetin components which are obtained through transformation from the marein. The coreopsis tinctoria extract has a certain effect of regulating intestinal flora, and can be used for related functional foods for improving metabolic diseases of high blood lipid, high blood pressure and high blood sugar.

Description

technical field [0001] The invention belongs to the field of medicines and functional foods, and relates to a preparation method and application of a snow chrysanthemum extract containing striacoside, more specifically, to a snow chrysanthemum extract containing sea chrysanthemum and its Application in the preparation and treatment of intestinal flora imbalance and metabolic diseases of hyperlipidemia, hypertension and hyperglycemia. Background technique [0002] Snow chrysanthemum is the flower of two-color coreopsis (Coreopsis tinctoria Nutt.), which is native to North America. It is "Guri Chayi". Folks in this area drink snow chrysanthemum as a special scented tea, which is believed to have the effects of lowering blood pressure, blood sugar and fat. With people's understanding and popularization of the efficacy of this medicinal material, snow chrysanthemum is known as a Xinjiang specialty with the same reputation as Tianshan snow lotus, and has been planted and promot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105
CPCA23V2002/00A23L33/105A23V2200/32A23V2200/326A23V2200/3262A23V2200/328A23V2250/21A23V2300/14A23V2300/10A23V2300/50
Inventor 李雅丽谢辉谭蓓高飞刘江云郝丽莉木胡牙提·乌拉斯汉
Owner SUZHOU UNIV
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