Immunomagnetic composition, its preparation method, use and kit for treating cancer

A composition and magnetic technology, applied in the direction of immunoglobulin, organic active ingredients, chemical instruments and methods, etc., can solve the problems of consumables, inability to circulate into the target area, limit dose, etc.

Active Publication Date: 2022-03-18
洪明奇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is time-consuming and consumable, and the cancer cells in the patient's body are prone to mutation, which makes the reinfused immune cells lose their original effect
On the other hand, the microbeads used for proliferation can only be used for in vitro culture due to their large size, and cannot enter the target area through human blood circulation
In addition, in addition to the surface-grafted antibodies or coated active ingredients, the materials forming the carrier are usually excipients, which are not very helpful for the treatment itself, and will limit the dose when administered. Inherent flaws in similar materials

Method used

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  • Immunomagnetic composition, its preparation method, use and kit for treating cancer
  • Immunomagnetic composition, its preparation method, use and kit for treating cancer
  • Immunomagnetic composition, its preparation method, use and kit for treating cancer

Examples

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preparation example Construction

[0084] 1.2 Preparation of Examples 1-3

[0085] The immunomagnetic composition of the present invention is further prepared under the aforementioned conditions for optimal preparation of IO@FuDex. The prepared IO@FuDex was grafted with the buffer solution (0.1M, pH=6) containing different antibodies and containing sodium cyanoborohydride (5μM) at 4°C for 4-6 hours, the aldehyde group in Dex The Schiff base will be formed with the primary amine on the antibody, and after the reductive amination reaction using sodium cyanoborohydride, the grafted antibody will reach a chemically stable state. Finally, the prepared immunomagnetic composition is purified using magnetic separation equipment. Please refer to Table 3 below, which are the antibodies used in Examples 1-3 of the present invention, wherein the antibodies used in Example 1 are CD3 antibodies and CD8 antibodies, and the antibodies used in Example 2 are PD-L1 antibodies. 3 The antibodies used are PD-L1 antibody, CD3 antib...

Embodiment 2 test 。 pic 11B Embodiment 2 and IgGIO@FuDex test 。 pic 11CIO@FuDex no. 1、4、12 and 24 and 4T1 pic 。 pic 11D Embodiment 2 no. 1、4、12 and 24 and 4T1 pic 。 pic 11E1.4μg/ml(；L)、7μg/ml(；M) and 35μg/ml(；H)CD3/CD28 Embodiment 3

[0098] Please refer to Figure 11A to Figure 11F , is an analysis result diagram of the targeting ability and cell binding ability of the immunomagnetic composition of the present invention. in Figure 11A In vitro targeting ability test of Example 2 of PD-L1 antibodies with grafting concentrations of 1.4 μg / ml (low concentration; L), 7 μg / ml (medium concentration; M) and 35 μg / ml (high concentration; H) result. Figure 11B It is the test result of the in vitro targeting ability of IO@FuDex grafted with IgG in Example 2. Figure 11C It is the analysis results of IO@FuDex binding ability to 4T1 cell line at 1, 4, 12 and 24 hours. Figure 11D It is the analysis result diagram of the binding ability of Example 2 to 4T1 cell line at 1, 4, 12 and 24 hours. Figure 11E Example 3 of CD3 antibody / CD28 antibody with a grafting concentration of 1.4 μg / ml (low concentration; L), 7 μg / ml (medium concentration; M) and 35 μg / ml (high concentration; H) in vitro targeting ability Test Re...

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Abstract

The invention discloses an immunomagnetic composition and its preparation method, application and cancer treatment kit. The immunomagnetic composition comprises a core layer, a shell layer and an outer layer. The shell layer is composed of a composite, and the shell layer covers the core layer. The complex is formed by the combination of fucoidan, oxidized dextran and superparamagnetic iron oxide nanoparticles through hydrophobic force. The outer layer contains at least one antibody, and the antibody is grafted outside the shell layer to form the outer layer. Thereby, the aforementioned immunomagnetic composition can be used for preparing anticancer drugs.

Description

technical field [0001] The present invention relates to a composition and its preparation method, in particular to an immunomagnetic composition characterized by a special physical form, its preparation method, use and a kit for treating cancer. Background technique [0002] Cancer, also known as malignant tumor, is a state of abnormal proliferation of cells, and these hyperplastic cells may invade other parts of the body. It is a disease caused by an abnormal mechanism of controlling cell division and proliferation. The number of people suffering from cancer is increasing all over the world. Cancer is one of the top ten causes of death in China, and it has been the top ten cause of death for 27 consecutive years. [0003] Conventional cancer treatment methods include surgery, radiation therapy and chemotherapy. Immunotherapy is another method of treating cancer other than the above-mentioned treatment methods. It activates the patient's own immune system and uses tumor cel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/395A61K31/715A61K41/00A61K45/06A61K47/36A61K47/02A61P35/00
CPCA61K39/395A61K39/3955A61K39/39566A61K41/00A61K45/06A61K47/02A61K47/36A61K31/715A61K2039/505A61K2039/507A61K2300/00A61K31/737A61P35/00A61K47/6923C07K16/00C07K16/2818C07K16/2809C07K16/2827A61K2039/804A61P35/04A61K2039/82A61K47/6929A61K47/6939A61K47/6849A61K47/6851
Inventor 徐伟成陈三元江智圣谢佳宏林佑融蔡长海
Owner 洪明奇
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