Culture medium for culturing lysurus mokusin and preparation method thereof

Abstract

The invention provides a culture medium for culturing lysurus mokusin, and belongs to the field of the microculture. Research shows that the lysurus mokusin has good pesticide development prospect. According to a plurality of current lysurus mokusin culture mediums for culturing fungi, the growth of mycelium is slow, a form of the mycelium is sparse and distorted, and cannot satisfy the requirements of an experiment. The invention provides the culture medium for culturing lysurus mokusin mycelium. A preparation method for the culture medium comprises the following steps: collecting raw materials of silver birch branches in two years old, fallen leaves of dahurian larch, fallen leaves of silver birch and the like, after treatment of smashing, an ultrasonic wave and the like, preparing an agar culture medium, sub-packaging in a culture dish, and enabling the lysurus mokusin mycelium to be inoculated on the culture medium for culturing. The culture medium is capable of accelerating a growth rate of the lysurus mokusin mycelium, wherein time of overgrowing in 9 mm of the culture dish is shortened from 14 days of a common fungus culture medium to 9 days. The culture medium is also suitable for isolating a tissue of a lysurus mokusin sporophore and culturing ysurus mokusin spores.

Description

technical field [0001] The invention belongs to the field of microorganism cultivation, and relates to a culture medium, in particular to a culture medium for cultivating five-row Sanwei phallus penis and a preparation method thereof. Background technique [0002] Lysurus mokusin belongs to the family Clathracrae and belongs to the genus Phallus. The head of Phallus pentaphyllis is slender, mostly pentagonal, with 4-5 lobes on the head, brick red, the initial lobes are connected to each other, and later they are separated from the top, and dark brown spore mucus is produced inside , with a fishy smell, often attracts flies and other insects. The stipe is light pink to light flesh-colored, with 4-5 grooves, spongy, and the receptacle is white. Also known as prismatic scattered tail ghost pen, five-sided scattered tail ghost pen. [0003] Studies have shown that the ethanol extract of Wulens Sanwei Phallus penis has a good antifeedant effect on gypsy moth larvae; n-butanol ...

AI Technical Summary

Problems solved by technology

Phallus pentaphyllum mycelium grows slowly on the above mediums, which cannot meet the requirements of the experiment