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miRNA markers associated with Crohn disease and application thereof

A technology for Crohn's disease and detection of Crohn's disease, applied in the direction of DNA/RNA fragments, recombinant DNA technology, biochemical equipment and methods, etc., can solve the problems of expression level and function that have not been reported, and achieve wide clinical application Foreground, stable result, simple operation effect

Active Publication Date: 2018-11-13
ZHENJIANG NO 1 PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, miR-219a-1-3p and miR-219a-5p were significantly higher in CD4 patients with CD + The expression level and effect in T cells have not been reported

Method used

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  • miRNA markers associated with Crohn disease and application thereof
  • miRNA markers associated with Crohn disease and application thereof
  • miRNA markers associated with Crohn disease and application thereof

Examples

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Embodiment 1

[0024] Diagnosis of miR-219a-1-3p and miR-219a-5p in Crohn's disease

[0025] 1. Compound: miR-219a-1-3p and miR-219a-5p specific reverse transcription and qRT-PCR primers.

[0026] 2. Composition:

[0027] (1) miRNA reverse transcription reagent: 5×Reverse Transcription Buffer, RTase Mix, RNase-free H 2 O, primers for reverse transcription.

[0028] (2) miRNA qRT-PCR reaction reagent: 2×SYBR Green Mix (Taq enzyme, dNTP mix, PCR Buffer, SYBR GreenⅠ); ddH 2 O; ROX dye; miR-219a-1-3p and miR-219a-5p specific qRT-PCR upstream primer (miR-219a-1-3p upstream primer sequence is shown in SEQ ID NO.3, miR-219a-5p The upstream primer sequence is shown in SEQ ID NO.4) and the universal downstream primer. The method is based on specific stem-loop RT primers and forward and reverse primers, and uses SYBRGreen dye for detection, without fluorescent probes, and has the advantages of high sensitivity and high specificity.

[0029] 3. Method:

[0030] (1) Peripheral blood CD4 + T cell ...

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Abstract

The invention discloses a set of miRNA markers associated with Crohn disease and an application thereof. The markers are miR-219a-1-3p and / or miR-219a-5p, and the expression level of the miR-219a-1-3pand / or the miR-219a-5p in the Crohn disease is detected by adopting qRT-PCR to provide reference value for clinical diagnosis and targeted therapy of the Crohn disease. The markers are high in accuracy, sensitivity and specificity when used for the clinical diagnosis of the Crohn disease, has the advantages of convenience and quickness, and avoids the limitation of colonoscopy to some extent.

Description

technical field [0001] The invention belongs to the technical field of medical molecular biology, and specifically relates to a group of miRNA markers related to Crohn's disease and applications thereof. Background technique [0002] Crohn's disease (CD) is a chronic, relapsing, nonspecific inflammatory disease involving the gastrointestinal tract. The etiology and pathogenesis of CD are still unclear, but more and more studies have shown that CD4 + The inflammatory immune response mediated by excessive activation of T cells plays a crucial role in the pathogenesis of CD. In recent years, the incidence of CD in my country has been increasing year by year. The disease is often protracted, prone to relapse, and has risks of disability and cancer, seriously affecting the quality of life of patients and causing a heavy medical and economic burden to the society. Therefore, elucidating the specific pathogenesis of CD and finding new targets that can be used for clinical diagnos...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6883C12Q2600/158C12Q2600/178C12Q2561/113C12Q2563/107C12Q2545/114C12Q2521/107
Inventor 石岩姚俊许亚平周勇王涛
Owner ZHENJIANG NO 1 PEOPLES HOSPITAL
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