Test paper strip for detecting tetrahydrocannabinolate as well as preparation method and application method thereof
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A technology of THC and test strips, which is applied in measuring devices, instruments, fluorescence/phosphorescence, etc., can solve the problems of inability to realize rapid detection of large batches of samples, complicated operation, and long time consumption, and achieve the elimination of non-specificity Fluorescent interference, simple operation, simple storage effect
Inactive Publication Date: 2018-11-13
郑州左安检测科技有限公司
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[0005] The purpose of the present invention is to overcome the existing methods for detecting THC that are complex in operation, time-consuming, and unable to realize the rapid detection of large batches of samples, and provide a method that is simple in operation, high in sensitivity, Test strips for detecting tetrahydrocannabinolic acid with fast detection speed and low cost and their preparation method and application method, so as to realize rapid detection and on-site monitoring of large quantities of tetrahydrocannabinolic acid samples
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[0041] The preparation method of the test strip for detecting THC mainly comprises the following steps:
[0042] 1) Label THC antibody with fluorescent microspheres and spray it on the conjugate release pad to prepare conjugate release pad sprayed with THC antibody labeled with fluorescent microspheres;
[0043] 2) Spray the hapten-carrier protein conjugate and goat anti-mouse secondary antibody on the reaction membrane as the test line (T) and quality control line (C) to prepare the THC-coated hapten- The detection line of the carrier protein conjugate and the reaction membrane of the quality control line coated with goat anti-mouse secondary antibody;
[0044] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the bottom plate into a test strip.
[0045] The following step-by-step detailed description:
[0046] 1. Preparation of fluorescent microsphere markers:
[0047]Preparation of ...
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Abstract
The invention discloses a test paper strip for detecting tetrahydrocannabinolate as well as a preparation method and an application method thereof. The test paper strip comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorbing pad and a substrate in sequence, wherein the conjugate release pad is coated with an antibody marked by time resolved fluorescence microspheres; the reaction membrane is coated with a detection line of hapten-carrier protein conjugate and a quality control line of a goat-anti-mouse second antibody. Due to adoption of the time resolved fluorescence marked antibody and an immunochromatography technique, rapid immunoassay of tetrahydrocannabinolate can be achieved. The invention further provides a method for detecting tetrahydrocannabinolate in a sample by using the test paper strip. The test paper strip has the advantages of being high in sensitivity, accurate in quantification, rapid in detection, convenient to operate, economical, and practical, and rapid detection and on-site detection on large scales of tetrahydrocannabinolate samples can be achieved.
Description
technical field [0001] The invention relates to the detection of tetrahydrocannabinolic acid, in particular to a test strip for quantitatively detecting tetrahydrocannabinolic acid by using time-resolved fluorescent microsphere immunochromatography technology and a preparation method and an application method thereof. Background technique [0002] Marijuana, which exists in cannabis leaves, has the functions of relieving cough, analgesia, and sleeping. It can be sucked, taken orally, and chewed, and it can produce a sense of spiritual pleasure and habit after taking it. If drug addicts inhale large doses of marijuana, they will have hallucinations, delusions, and paranoid states, accompanied by disordered thinking, self-awareness disorders, and dual personality. Long-term use may cause insomnia, loss of appetite, irritability, irritability, vomiting, trembling, poor comprehension and memory, decreased immunity, and easy to get various diseases, which will make the body weak...
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