A kind of culture medium and culture method of hydroponic propagation of Daye Jinwa

A culture medium and hydroponic technology, which is applied in the field of culture medium for hydroponic propagation, can solve problems such as shortage of golden lumbar resources, and achieve the effects of shortening rooting time, high transplant survival rate, and improving propagation ability and propagation coefficient.

Active Publication Date: 2020-03-27
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, my country's golden waist resources are in short supply, and domestic and foreign research on the hydroponic rapid propagation technology of golden waist, especially the hydroponic rapid propagation technology of Daye golden waist, can be said to be blank

Method used

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  • A kind of culture medium and culture method of hydroponic propagation of Daye Jinwa
  • A kind of culture medium and culture method of hydroponic propagation of Daye Jinwa
  • A kind of culture medium and culture method of hydroponic propagation of Daye Jinwa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The hydroponic propagation culture medium of the explant of the golden loin of big leaf of embodiment 1

[0031] In order to explore the effect of different culture medium composition on the hydroponic propagation effect of the velvety golden loin explants, we designed an experiment with three change factors as the research object, one is the type of culture medium added, and the other is the added hormone. The third is the mass concentration of the added hormone. There are 18 types of culture medium used in the experiment, all of which use tap water as a solvent.

[0032] The preparation method of the culture solution is to prepare according to the formula in Table 1, using the conventional methods in the art.

[0033] The specific components of the 18 culture solutions are shown in the table.

[0034] Table 1 Types of culture medium for hydroponic propagation of Dayejinwa explants

[0035]

Embodiment 2

[0036] Embodiment 2: the method for the hydroponic propagation of the explant of Rhizoma japonica

[0037] In order to improve the stringency of the culture results, during the culture process, 18 kinds of culture solutions were prepared in Example 1, and each culture solution was set up with 6 replicates, all of which were cultured in a petri dish, so as to observe the culture effect of a single explant.

[0038] The concrete method of the hydroponic propagation of the explant of Daye Jinwa is as follows:

[0039] (1). Preparation of various types of hydroponic breeding culture fluid described in Example 1;

[0040] (2). The selection of explants, select the plants with relatively strong growth, no pests and diseases, and suitable growth stages, and take the young and tender leaves of the plants as explants;

[0041] (3). Handling and picking of explants: hold the plant with the left hand, and cut the petiole obliquely with the right hand to obtain the desired leaves; cleani...

Embodiment 3

[0043] Embodiment 3: The influence of different culture fluids on overall culture effect

[0044] During the culture of the explants, their growth status was observed every day, and photographed and recorded. pass figure 2 From the results shown, it can be seen that after the exogenous plants (leaves) were cultured in the Y-type culture solution for a few days, the surface of the plants had changes and bacterial contamination, and the growth status was obviously poor. After culturing until the 8th day, the wilting area of ​​the leaves expanded, and the growth conditions were not good, and the results of the 6 repeated experiments were the same. However, the explants in the X-type culture medium have been growing well and kept fresh and tender, such as figure 1 , and the results of the 6 repeated experiments were the same. In the control medium, the explants did not grow, although they remained green.

[0045] According to the observation results of different culture solut...

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Abstract

The invention relates to a culture solution for hydroponic propagation of chrysosplenium macrophyllum and a water culture method. According to the invention, a chrysosplenium macrophyllum leaf is adopted as the explants, and different culture solutions are used for culture, and according to whether the explants can generate adventitious root and axillary bud and the length of time needed, a culture solution suitable for hydroponic propagation of chrysosplenium macrophyllum is selected and transplant to the outdoor. The culture method provided by the invention can achieve high transplanting survival rate, greatly promotes the propagation of chrysosplenium macrophyllum, effectively shortens the rooting time, can provide the raw material source for large-scale planting and field management ofchrysosplenium, and is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of hydroponic propagation of plants, and in particular relates to a culture solution and a cultivation method for the hydroponic propagation of Rhododendron japonicus. Background technique [0002] Chrysosplenium is a plant belonging to Saxifragaceae (Saxifragaceae). There are about 65 species in the world. They are distributed in Asia, Europe, Africa, and America, but they are mainly produced in the temperate zone of East Asia. Generally, it is divided into Subg.Gamosplenium and Subg.Chrysosplenium or Subg.Dialysplenium according to the opposite or alternate leaves: there are about 33 species of Subg.Gamosplenium in the world, and 23 species and 6 varieties are produced in China; There are about 32 species in the genus Lumosa in the world, 11 species and 2 varieties in China; there are about 51 species in East Asia, and 36 species and 8 varieties in China, both in the north and the south, and 21 of them are...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张丽刘虹覃瑞刘秤利张丹丹李媛媛庞熊翠赖奕亦
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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