Method for extracting quercitrin and bergenin from herba ardisiae japonicae

A technology of rock cabbage and dwarf tea, which is applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems that dwarf tea has not been comprehensively developed and utilized, and can shorten the extraction time and improve the extraction efficiency. rate, and the effect of increasing added value

Active Publication Date: 2018-11-20
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Aidi tea has not been comprehensively developed and utilized, and it is of great significance to explore the simultaneous extraction of quercetin and pethenin from Aidi tea

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Crush 100 kg of Aodi tea to 100 mesh, add 300 L of diethyl ether, carry out reflux extraction at 50 ° C for 100 min, and filter to obtain filtrate I and filter residue I. Add filter residue I to 10L of Trichoderma fermentation broth, ferment at 30°C for 18 hours, then add 15L of Aspergillus niger fermentation broth to it, ferment at 30°C for 2 days, then add 300L of ethanol solution, carry out reflux extraction at 85°C for 60min, and filter to obtain 280L of filtrate II . The filtrate II was concentrated under reduced pressure at 50°C to obtain 5L concentrated liquid I, which was mixed with 5L methanol evenly, put on an AB-8 macroporous resin column, and eluted with a solution containing 30% methanol five times the volume of the column to obtain 80L elution solution. Eluent I was stripped, and the eluent I was concentrated under reduced pressure at 50°C to obtain 3L of concentrated solution II. Continue elution with six column volumes of 80% methanol solution to obtain...

Embodiment 2

[0034] Crush 500 kg of Aodi tea to 100 mesh, add 1000 L of diethyl ether, carry out reflux extraction at 50 ° C for 100 min, and filter to obtain filtrate I and filter residue I. Add filter residue I to 60L of Trichoderma fermentation broth, ferment at 30°C for 20 hours, then add 60L of Aspergillus niger fermentation broth to it, ferment at 30°C for 3 days, then add 1200L of ethanol solution, carry out reflux extraction at 85°C for 60min, and filter to obtain 1160L of filtrate II . The filtrate II was concentrated under reduced pressure at 50°C to obtain 25L of concentrated liquid I, which was mixed with 25L of methanol evenly, put on an AB-8 macroporous resin column, and eluted with a solution containing 35% methanol five times the volume of the column to obtain 400L of concentrated solution I. Eluent I was stripped, and the eluent I was concentrated under reduced pressure at 50°C to obtain 18L of concentrated solution II. Continue elution with six column volumes of 80% meth...

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Abstract

The invention provides a method for extracting quercitrin and bergenin from herba ardisiae japonicae. The method comprises specific steps as follows: the herba ardisiae japonicae is crushed, ethyl ether is added for reflux extraction, filtration is performed, and a filtrate I and filter residues I are obtained; the filter residues I are added to a trichoderma fermentation broth and an aspergillusniger fermentation broth for fermentation, ethanol is added for reflux extraction, filtration is performed, and a filtrate II is obtained; the filtrate II is concentrated under reduced pressure and then mixed with methanol, the mixture is loaded on a macroporous resin column, elution is performed with methanol, and an eluate is obtained; the eluate is concentrated under reduced pressure and left to stand overnight at 4-8 DEG C, crystallization and recrystallization are performed, and pure bergenin and quercitrin are obtained.

Description

technical field [0001] The invention belongs to the technical field of natural product chemistry, and relates to a method for separating and purifying quercetin and petracenin from tea tea. Background technique [0002] Aodicha is the dried whole plant of Zijinniu, a plant of the family Zijinbu. The whole plant is 21.5-25 cm long. Mostly with creeping rhizomes. Stems cylindrical or slightly flattened, 2-5mm in diameter, dark reddish brown on the surface, with fine longitudinal lines and protruding leaf scars, sparsely fibrous adventitious roots at the base; pedicel or dark red shrunken spherical small fruit sometimes visible at the top, producing in Hunan Province. The whole plant is used as medicine. Main ingredients: purple gold oxol Ⅰ, Ⅱ, quercetin, petracenin, letter box quinone, wintergreen terpene alcohol, acid taurine quinone and quercitrin, volatile oil, myricetin, etc. The whole plant also contains 11-O-vanilloyl petracenin, 11-O-syringoyl pyroglyllin, 11-O-gal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08C07D493/04
CPCC07D493/04C07H1/08C07H17/07
Inventor 周喜新
Owner HUNAN AGRICULTURAL UNIV
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