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Application of Bacillus megaterium bm22 and its spore liquid preparation in the control of cyclamen blackroot disease

A kind of Bacillus megaterium, technology of liquid preparation

Active Publication Date: 2020-08-18
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the disease is mainly controlled by chemicals, but the biological control that is environmentally friendly, safe for humans and animals, and sustainable use has not involved

Method used

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  • Application of Bacillus megaterium bm22 and its spore liquid preparation in the control of cyclamen blackroot disease
  • Application of Bacillus megaterium bm22 and its spore liquid preparation in the control of cyclamen blackroot disease
  • Application of Bacillus megaterium bm22 and its spore liquid preparation in the control of cyclamen blackroot disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Isolation and Identification of Bacillus megaterium BM22

[0028] 1.1 Isolation, purification and preservation of endophytic bacteria

[0029] Healthy leaves of Eucommia ulmoides were collected from the black spot occurrence area of ​​Eucommia ulmoides in Dayi, Sichuan. Rinse the collected samples with sterile water, weigh 1.0g of tissue, soak in 70% alcohol for 1-2min, then disinfect with 1-3% sodium hypochlorite solution for 3-5min, wash several times with sterile water, absorb Apply 100 μL of washing liquid for the last time to NA plate (3g beef extract, 10g peptone, 5g sodium chloride, 15-20g agar powder, 1000mL distilled water, pH 7.0, after mixing and dispensing, autoclave at 121°C for 30min), culture in the dark at 27°C 24h, used as a control for surface disinfection, to check whether the surface disinfection is thorough.

[0030] Cut the surface-sterilized bark tissue into pieces, put them into a sterile mortar, add sterilized quartz sand and 10mL st...

Embodiment 2

[0046] The preparation of embodiment 2 bacillus megaterium BM22 spore liquid agent

[0047] 1.1 Preparation of spore liquid preparation

[0048] The Bacillus megaterium BM22 obtained according to Example 1 is made into a liquid preparation through seed culture and fermentation culture, specifically through the following methods:

[0049] The conventional method is to prepare beef extract peptone slant medium (beef extract 5g, peptone 10g, NaCl 3.5g, agar 18g, water 1000mL), inoculate Bacillus megaterium, and cultivate to make first-class seeds.

[0050] Nutritious succulent culture solution (beef extract 7g, peptone 10g, NaCl 5g, magnolia officinalis root bark soaking solution 1000mL) was autoclaved, and under oxygen control, bottled according to the proportion of 100mL liquid in a 300mL triangular flask, Inoculate Bacillus megaterium slant seeds in the state, inoculate 2 slant seeds per bottle, vibrate and cultivate, and make Bacillus megaterium liquid seeds. Wherein, 10 g ...

Embodiment 3

[0053] Embodiment 3 root irrigation application disease prevention and growth promotion experiment

[0054] According to the degree of disease, in spring, dilute the BM22 spore liquid preparation to different concentrations to irrigate the soil 100mL / plant once each time, and count the results after 30 days. For control effect and growth promotion see (Table 3-6). For mild occurrence, the economic concentration is 1000 times the solution, for moderate occurrence, the economic concentration is 800 times the solution, and for severe occurrence, the economic concentration is 50 times to the stock solution.

[0055] Table 3 BM22 spore liquid preparation biocontrol cyclamen blackroot disease

[0056]

[0057] Table 4 BM22 strains promote the growth of cyclamen (1-year-old seedlings, mild occurrence area)

[0058]

[0059] Table 5 BM22 strains promote the growth of cyclamen (1-year-old seedlings, moderate occurrence area)

[0060]

[0061]

[0062] Table 6 BM22 strain...

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Abstract

The invention discloses the application of Bacillus megaterium BM22 and its spore liquid preparation in the prevention and treatment of cyclamen blackroot disease. The Bacillus megaterium BM22 has been preserved, and the preservation unit is: China Ordinary Microorganism Culture Preservation Management Center, preservation number It is CGMCC No.15070. The bacillus megaterium BM22 spore liquid preparation is obtained from the bacillus megaterium BM22 as the strain, and the liquid preparation is obtained through seed cultivation and fermentation culture. The bacillus megaterium BM2 of the present invention has spores with strong stress resistance, is suitable for commercial production, and has dual functions of disease prevention and growth promotion.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to the application of bacillus megaterium BM22 and its spore liquid preparation in the prevention and treatment of cyclamen blackroot disease. Background technique [0002] Cyclamen persicum Mill, also known as radish crabapple, rabbit ear flower, rabbit flower, Yipinguan, bonfire flower, and petal lotus, is a perennial herb of Primulaceae and cyclamen. The leaves grow from the top of the tuber, and the heart Shaped, ovate or reniform, the leaves have fine serrations, the leaf surface is green, with white or gray halo spots, the back of the leaves is green or dark red, the petiole is long, reddish brown. Cyclamen likes warmth and is afraid of heat. It grows best in a cool environment and fertile sandy loam rich in humus. It is more cold-resistant and can withstand low temperatures of 0°C without being frozen. Autumn to spring of the second year is its growing season. It is se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/11
CPCA01N63/00C12N1/20C12N1/205C12R2001/11
Inventor 朱涵明月朱天辉
Owner SICHUAN AGRI UNIV
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