Microbial strain used for treating heavy metal contaminated soil as well as screening method and application thereof

A technology of polluted soil and screening method, which is applied in the field of microbiology, can solve problems such as threats to the production and life of nearby residents, unsatisfactory repair effects, and pollution from chromium slag piles, and achieve low cost, strong tolerance, and good results

Active Publication Date: 2018-11-23
苏州逸凡特环境修复有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In 2011, a chromium slag pollution incident occurred in Qujing, Yunnan, China. The investigation found that the groundwater in the "Longtan" area on the southeast side of the Yunnan Luliang Chemical Industry Co., Ltd. plant may have been severely polluted, and the chromium at the groundwater outlet in this area exceeded the standard by 242 times. The chromium in the water in the field exceeds the standard by 126 times, and the water in the nearby river section is used for agricultural irrigation, which seriously threatens the production and life of nearby residents
At present, most of the restoration technologies that have received attention are in the stage of laboratory experiments, and the actual restoration effect is not ideal.

Method used

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  • Microbial strain used for treating heavy metal contaminated soil as well as screening method and application thereof
  • Microbial strain used for treating heavy metal contaminated soil as well as screening method and application thereof
  • Microbial strain used for treating heavy metal contaminated soil as well as screening method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0028] The present invention is used for the screening of the microorganism bacterial strain that heavy metal polluted soil processes adopts liquid phase enrichment method, comprises the following steps:

[0029] (1) Weigh 5g of contaminated soil samples (the soil is taken from Shangfangshan, Suzhou City, and finally contains Cd 2+ 25mg / kg, Cr 6+ 50mg / kg), join in the liquid culture medium that beef extract peptone is housed, shake at 30 ℃, 160rpm then, obtain soil mixture;

[0030] (2) the soil mixture is transferred to the centrifuge tube, and the supernatant after the centrifugation is taken as the source of cadmium and chromium passivation microorganisms;

[0031] (3) Take 5mL of the acclimatized suspension and apply it evenly on Cd by dilution coating method. 2+ and Cr 6+ Concentrations were 25mg / L and 50mg / L respectively on solid medium plates, placed in a 37°C constant temperature incubator and cultured upside down for 48 hours, and selected dominant single colonies ...

Embodiment 2

[0040] The microbial strains screened by the present invention are inoculated in the beef extract peptone liquid medium (the inoculum size is 2wt%) sterilized at 121°C for 30min, cultivated (160rpm, cultivated at 30°C for 12h) to the logarithmic growth phase, and the bacterial liquid is obtained .

[0041] The bacterial solution can be added to the contaminated soil containing heavy metals cadmium and chromium, mixed evenly, cultivated for about 15 days, using the BCR continuous extraction method to extract various forms of cadmium in the soil; and wash the soil with water to determine the amount that can be absorbed and utilized by plants. Exchangeable cadmium, detected by ICP-AES.

[0042] Or freeze-dry the bacterial liquid with a vacuum freeze dryer and add it to the contaminated soil containing heavy metal cadmium, mix it evenly, cultivate it for about 15 days, use the BCR continuous extraction method to extract various forms of cadmium in the soil; wash the soil with wate...

Embodiment 3

[0045] Add different concentrations of Cd to beef extract peptone broth 2+ and Cr 6+ (Cd of 10, 20, 40, 60mg / L 2+ , corresponding to Cr containing 20, 40, 60, 100mg / L 6+ ), sterilized at 121°C for 30min; then inoculated with the microbial strains screened by the present invention, cultivated at 30°C and 160rpm, and took samples at different times to measure the OD value; at the same time, the concentrations were 10, 20, 40, 60mg / L and 20, 40mg / L respectively , 60, 100mg / L of Cd 2+ and Cr 6+ At 0.5, 4, 8, and 12 hours, the strain enters the logarithmic growth phase, and the highest OD values ​​that can be achieved are 6.1, 5.5, 3.2, and 2.1, respectively. The data show that the strain has a strong resistance to Cd 2+ and Cr 6+ Has a strong tolerance.

[0046] The above inoculation of the microbial strains screened by the present invention is to get 2wt% fresh bacterial liquid, or to pick a single colony on the beef extract peptone solid medium and insert it into the mediu...

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Abstract

The invention discloses a microbial strain used for treating heavy metal contaminated soil as well as a screening method and application thereof. A screened microbial strain is bacillus subtilis withthe collection number of CCTCC NO:M 2017834. An application method of the microbial strain comprises the following steps: inoculating the microbial strain in a culture medium for culturing to a logarithmic growth phase to obtain a bacteria solution; adding the bacteria solution into the heavy metal contaminated soil, and uniformly mixing to finish the treatment of the heavy metal contaminated soil; or adding the bacterial solution which is freeze-dried in vacuum into the soil, and mixing uniformly to finish the treatment of the heavy metal contaminated soil; fixing cadmium and chromium in thesoil under the action of inactivation in order to lower the bio-availability of the cadmium and the chromium, and lower the absorbing effect of plants on the cadmium and the chromium in order to fulfill the aim of repairing soil.

Description

technical field [0001] The invention relates to the field of microorganisms, and relates to a microbial strain for remediating heavy metal-contaminated soil, a screening method and application thereof. Background technique [0002] Heavy metals refer to metals with a specific gravity greater than 5. Currently, there are about 45 types, such as copper, lead, zinc, iron, cobalt, nickel, manganese, cadmium, mercury, tungsten, molybdenum, gold, silver, etc. A small amount of heavy metals such as copper, manganese and zinc are elements necessary for life activities, but most heavy metals such as cadmium, mercury, lead, chromium and other elements are not necessary for organisms, and excessive heavy metal elements are toxic to the human body . Heavy metals are widely distributed in the atmosphere, lithosphere and hydrosphere. Under natural conditions, their concentrations generally do not reach the level of harming the environment and human beings. However, with the development ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02B09C1/10C12R1/07
CPCB09C1/10C12N1/02C12N1/20C12N1/205C12R2001/07
Inventor 蔡慧王佳斌贺志刚程刚
Owner 苏州逸凡特环境修复有限公司
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