A kind of culture medium and culture method for culturing astrocytes in vitro

Abstract

The invention discloses a culture medium and a culture method for culturing astrocytes in vitro, belonging to the field of cell culture. In the present invention, by adding epidermal growth factor, fibroblast growth factor, SAG and Purmorphamine to the astrocyte culture medium, the in vitro culture time of astrocytes can be significantly prolonged, the number of passages can be increased, the purity can be improved, and the purity can be maintained. Inherent properties of astrocytes. The inventors have found that the culture medium of the present invention can be used at alternate generations and maintain astrocytes for up to 13 passages. The purity of astrocytes cultured to passage 12 is >95%, and the original characteristics of astrocytes can be maintained.

Description

technical field [0001] The invention belongs to the field of cell culture, and in particular relates to a culture medium and a culture method for culturing astrocytes in vitro. Background technique [0002] Astrocytes are a type of glial cells that spread throughout the central nervous system and engage in multiple complex functions. They exchange substances with neurons and play an indispensable role in the growth of neurons. The main basis for identifying astrocytes is that they can express a glial fibrillary acidic protein (GFAP). In the central nervous system, Reactive astrocytes respond to various injuries and eventually form glial scars. In biology, it is often used to judge the damage site of the central nervous system. Astrocytes participate in the blood-cerebrospinal fluid barrier, have a certain regulatory effect on the blood flow of the central nervous system, and then affect the activity of neurons; astrocytes connect with synapses and play an important role in...

AI Technical Summary

Problems solved by technology

However, this method has little effect on cells that have been passaged for many times, and cells that lose division activity during the passage process cannot regain division properties by adding dBcAMP

At the same time, the cost of this culture method is relatively high, and the operation process is complicated.

[0004] In summary, the current methods for culturing astrocytes are complex and the cells grow slowly. Generally, the purity of astrocytes is 75-95% after in vitro culture until the third passage.

Operations such as changing the medium many times during the experiment not only increase the cost of the experiment, but also are not conducive to the growth of cells in a relatively stable environment, increasing the possibility of bacterial contamination

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