Cadmium sulfide quantum dot biosynthesis method, prepared cadmium sulfide quantum dots and application of cadmium sulfide quantum dots

A biosynthesis, quantum dot technology, applied in chemical instruments and methods, luminescent materials, fermentation, etc., can solve the problems of inability to reasonably control the synthesis size of quantum dots, poor quality of quantum dots, irregular shape, etc., to avoid bacteria. The effect of swarm pollution, good quantum yield and easy operation

Active Publication Date: 2018-11-23
THE FIFTH AFFILIATED HOSPITAL SUN YAT SEN UNIV
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Problems solved by technology

However, in the literature, the reaction cannot be terminated at any time, and ultrapure water dialysis is required to obtain quantum dots. During the reaction, the functions of smCSE mainly include two aspects: synthesizing cadmium sulfide and mediating the growth of quantum dots. Under water dialysis, the growth of quantum dots cannot be terminated, and the solution will eventually become a fluorescence-quenched cadmium su

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  • Cadmium sulfide quantum dot biosynthesis method, prepared cadmium sulfide quantum dots and application of cadmium sulfide quantum dots
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  • Cadmium sulfide quantum dot biosynthesis method, prepared cadmium sulfide quantum dots and application of cadmium sulfide quantum dots

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Embodiment 1

[0057] Embodiment 1 of the present invention is: a biosynthesis method of cadmium sulfide quantum dots, including experimental group 1 and control group 1, wherein the operation of experimental group 1 includes the following steps: 1) using L-cys as the S source, and using CdAc As a Cd source, cadmium sulfide quantum dots were synthesized using smCSE as a biocatalyst and trypsin as a reaction terminator. Specifically, take a certain amount of ultrapure water, add 1mol / L Tris buffer solution (pH=8.0), 0.1mol / L L-cys (purchased from Sigma vetec, analytically pure) solution, 0.01mol / L In the CdAc (purchased from Sigma Aldrich, analytically pure) solution and the smCSE protein, the amount of the certain amount of ultrapure water was calculated before the experiment to avoid excessive initial concentration and too fast reaction. The final concentration of smCSE in the reaction system is 0.1mg / ml, the final concentration of Tris is 50mmol / L, the final concentration of L-Cys is 10mmo...

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Abstract

The invention discloses a cadmium sulfide quantum dot biosynthesis method, prepared cadmium sulfide quantum dots and application of the cadmium sulfide quantum dots. The biosynthesis method comprisesthe following steps of by taking sulfur-containing amino acid as an S source, water-soluble cadmium salt as a Cd source, smCSE as a biocatalyst and protease as a reaction terminator, performing synthesis to prepare the cadmium sulfide quantum dots, and applying the cadmium sulfide quantum dots prepared in the step to the field of cell labeling. Compared with the prior art, the synthesis method provided by the invention is environmentally friendly and is strong in controllability; and the cadmium sulfide quantum dots prepared by the method is regular in shape, high in yield and good in stability and can be used for cell labeling or chemical detection.

Description

technical field [0001] The invention relates to the field of nanomaterial preparation, in particular to a biosynthesis method of cadmium sulfide quantum dots, the prepared cadmium sulfide quantum dots and applications thereof. Background technique [0002] Quantum Dots (QDs) are semiconductor nanocrystals, which have some unique fluorescent properties due to the size effect and quantum confinement effect of nanomaterials. [1-3] , such as controllable fluorescence emission wavelength, narrow and symmetrical emission peak, wide excitation wavelength range, high quantum efficiency, and good photostability, etc. Therefore, quantum dots have attracted extensive attention as an ideal fluorescent reporter group for immunoassays. However, the current synthesis method mainly adopts the traditional chemical synthesis method, which is not green and environmentally friendly. [0003] Chinese invention patent CN102965406B discloses a biosynthesis method of cadmium sulfide quantum dots. ...

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Application Information

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IPC IPC(8): C12P3/00C09K11/56
CPCC09K11/565C12P3/00
Inventor 陈守登王宇桐黄钊霞
Owner THE FIFTH AFFILIATED HOSPITAL SUN YAT SEN UNIV
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