Treatment method for breast cancer axillary lymph nodes dissection tissue specimen
A tissue specimen and processing method technology, which is applied in the preparation, sampling, and measurement devices of test samples, can solve the problems of insufficient sampling, affecting the patient's treatment plan, prognosis and outcome, and long operation time, so as to increase the throughput. permeability, improve work efficiency and material quality, and enhance the effect of permeability
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Embodiment 1
[0019] Embodiment 1: different compositions of ointment fixative
[0020] The core functional components of the palmitate fixative solution provided by this application are mainly formaldehyde, lecithin, absolute ethanol and acetone, and in different implementation environments, deoxycholic acid, methanol, PBS solution and any One or more, the specific composition selection method can refer to Table 1.
[0021] Table 1 different compositions of ointment fixative
[0022]
[0023]
[0024] We tested the core components of the ointment fixative (i.e. No. 1 in Table 1) and supplementary components respectively, and the above 16 situations can be obtained. The same components were tested in parallel with the same samples at the same concentration, and the results showed that: The soft lipid fixative of the core component has a good fixation effect on adipose tissue, organ tissue (such as thyroid tissue, breast tissue, lung tissue) or colon tissue, and the lymph nodes are we...
Embodiment 2
[0027] Embodiment 2: the same composition different concentrations of ointment fixed solution
[0028] The present embodiment provides the composition and concentration of the palmitate fixative solution of the whole composition (i.e. including all core constituents and all supplementary constituents), and several representative groups are listed as shown in Table 2:
[0029] Table 2 different concentrations of palmitate fixative
[0030]
[0031] The pretreated surgically resected omentum specimens were treated with the omentum fixative solution corresponding to the serial numbers in Table 2 for 4 hours, and then the lymph nodes were collected, dehydrated, embedded, sectioned, stained, immunohistochemical, and observed under a microscope. The results showed that: The above-mentioned concentrations can dissolve the sample to varying degrees, the fat is soft and liquefied, the lymph nodes are effectively exposed, the cell morphology of the lymph nodes is preserved intact, an...
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