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Treatment method for breast cancer axillary lymph nodes dissection tissue specimen

A tissue specimen and processing method technology, which is applied in the preparation, sampling, and measurement devices of test samples, can solve the problems of insufficient sampling, affecting the patient's treatment plan, prognosis and outcome, and long operation time, so as to increase the throughput. permeability, improve work efficiency and material quality, and enhance the effect of permeability

Inactive Publication Date: 2018-11-30
SHAOXING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For radical tumor resection specimens such as gastric cancer, colon cancer, and breast cancer, thorough lymph node sampling is crucial for tumor diagnosis, staging, formulation of treatment plans, and prognosis, but conventional fixative solutions and existing specimen fixation methods cannot meet the requirements. Improving the sampling speed and detection rate of lymph nodes while fast fixing allows pathologists to search for lymph nodes in the long-term isolated adipose tissue. This method has many disadvantages such as insufficient sampling and long operation time. Ultimately affect the patient's treatment plan, prognosis and outcome

Method used

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  • Treatment method for breast cancer axillary lymph nodes dissection tissue specimen
  • Treatment method for breast cancer axillary lymph nodes dissection tissue specimen
  • Treatment method for breast cancer axillary lymph nodes dissection tissue specimen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: different compositions of ointment fixative

[0020] The core functional components of the palmitate fixative solution provided by this application are mainly formaldehyde, lecithin, absolute ethanol and acetone, and in different implementation environments, deoxycholic acid, methanol, PBS solution and any One or more, the specific composition selection method can refer to Table 1.

[0021] Table 1 different compositions of ointment fixative

[0022]

[0023]

[0024] We tested the core components of the ointment fixative (i.e. No. 1 in Table 1) and supplementary components respectively, and the above 16 situations can be obtained. The same components were tested in parallel with the same samples at the same concentration, and the results showed that: The soft lipid fixative of the core component has a good fixation effect on adipose tissue, organ tissue (such as thyroid tissue, breast tissue, lung tissue) or colon tissue, and the lymph nodes are we...

Embodiment 2

[0027] Embodiment 2: the same composition different concentrations of ointment fixed solution

[0028] The present embodiment provides the composition and concentration of the palmitate fixative solution of the whole composition (i.e. including all core constituents and all supplementary constituents), and several representative groups are listed as shown in Table 2:

[0029] Table 2 different concentrations of palmitate fixative

[0030]

[0031] The pretreated surgically resected omentum specimens were treated with the omentum fixative solution corresponding to the serial numbers in Table 2 for 4 hours, and then the lymph nodes were collected, dehydrated, embedded, sectioned, stained, immunohistochemical, and observed under a microscope. The results showed that: The above-mentioned concentrations can dissolve the sample to varying degrees, the fat is soft and liquefied, the lymph nodes are effectively exposed, the cell morphology of the lymph nodes is preserved intact, an...

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Abstract

The application relates to a treatment method for a breast cancer axillary lymph nodes dissection tissue specimen and belongs to the technical field of specimen preparation for detection. The method includes: selecting a to-be-treated in-vitro breast cancer lymph nodes dissection tissue specimen, performing adipocyte permeability enhancement treatment and then performing fat softening treatment ina stationary liquid at room temperature for 2-6 h, cutting a lymph node, performing regular dehydration, and embedding, slicing and staining the sample to complete the treatment. The stationary liquid includes formaldehyde, lecithin, anhydrous ethanol and acetone according to the concentrations of 50-150 ml / L of the formaldehyde, 30-70 g / L of the lecithin, 200-300 ml / L of the anhydrous ethanol and 200-300 ml / L of the acetone. The treatment method is applied to a breast cancer lymph nodes dissection tissue specimen, and not only can effectively soften and dissolve fat tissue and guarantee timely and complete immobilization of the lymph nodes but also can effectively increase detection number of lymph nodes, make lymphatic metastasis positive rate accurate, and increase work efficiency of sampling operators.

Description

technical field [0001] The application relates to a method for processing breast cancer axillary lymph node dissection tissue samples, and belongs to the technical field of test sample preparation. Background technique [0002] Existing fresh isolated specimens are usually soaked in formalin fixative solution, mainly because formaldehyde can preserve the morphological and structural characteristics of tissues for a long time and has good economic utility at the same time. Although this type of specimen processing method can well fix the shape of the tissue, it also hardens the specimen containing adipose tissue, which greatly increases the difficulty in obtaining materials in pathology departments, especially lymph nodes. For radical tumor resection specimens such as gastric cancer, colon cancer, and breast cancer, thorough lymph node sampling is crucial for tumor diagnosis, staging, formulation of treatment plans, and prognosis, but conventional fixative solutions and exist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N1/28
CPCG01N1/28G01N1/30G01N2001/2873G01N2001/305
Inventor 陈冠颖沈增丽张小娟周春燕张剑孙爱静
Owner SHAOXING UNIVERSITY
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