A kind of Taishan smallpox extract and its application in the preparation of antipharyngitis medicine
A technology of extract and smallpox, which is applied in the field of preparation of anti-pharyngitis drugs, can solve the problems of short shelf life, dry products are easy to be eaten by insects, and lose the effect of pharyngitis, and achieve the effect of simple preparation method
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Embodiment 1
[0048] The preparation of embodiment 1 Taishan smallpox extract
[0049] (1) Take 100g of T. chinensis fruiting bodies, pulverize them with a pulverizer, extract with deionized water at 4°C for 12 hours at a solid-liquid ratio of 1:10, then centrifuge at 8000rpm for 20 minutes, and take the supernatant.
[0050] (2) Precipitate the supernatant with 70% ammonium sulfate. After 4 hours of precipitation, centrifuge at 1000 rpm for 15 minutes. Discard the supernatant and keep the precipitate.
[0051] (3) Dissolve the precipitate with deionized water, and then dialyze with a dialysis membrane with a molecular weight of 3kD for 10 hours. During this period, the deionized water is changed twice to remove polyphenols.
[0052] (4) Add the extract obtained by dialysis to the extract with 1M pH 3.54 acetic acid-sodium acetate buffer solution at a ratio of 1:10, and precipitate the protein with the corresponding isoelectric point in the extract, and centrifuge at 12000rpm After 10 minu...
Embodiment 2
[0060] Embodiment 2 Pharyngeal Histopathological Observation
[0061] In this embodiment, SD rats were selected as the research objects, and were randomly divided into model group, blank control group, natural recovery group, positive control group and active substance P high, medium and low dose groups. The modeling method is as follows: inject about 10 8 cfu / ml of Staphylococcus aureus, the injection volume is 0.02ml each, once a day for 3 consecutive days. The administration method is as follows: on the 4th to 10th day, spray the active substance P to the pharyngeal mucosa of the rat with a throat sprayer, and the dosages are 35mg / kg, 17.5mg / kg, and 8.75mg / kg, and spray once every 2 hours , twice in the morning and afternoon, spray 4 times a day. The positive control group used amoxicillin suspension (18mg / kg). On the 11th day, the serum of the rats in each group was taken for future use, and the pharyngeal tissues of the rats were taken after the cervical spine was kille...
Embodiment 3
[0070] The mensuration of embodiment 3 biochemical indexes
[0071] Rat interleukin-1β (IL-1β), interleukin-6 (IL-6), intercellular adhesion factor (ICAM-1), C-reactive protein (CRP), tumor necrosis factor-α (TNF-α) ELISA The kit (Shanghai Lanji Biotechnology Co., Ltd.) detects the content of inflammatory factors in the rat serum prepared in Example 2, and the test results are as follows:
[0072] 1) CRP measurement results
[0073] The CRP in the serum of rats in the model group was significantly higher than that in the blank control group, with a very significant difference (P0.05).
[0074] Table 1 The effect of the water extract of Taishan Tianhua on the CRP in the serum of rats with acute bacterial pharyngitis (X ± S)
[0075]
[0076] Note: Compared with the blank control group: ◆ is P<0.05, ◆◆ is P<0.01; compared with the model group: * is P<0.05, ** is P<0.01
[0077] 2) IL-1β measurement results
[0078] Compared with the blank control group, the IL-1β in the s...
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