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Preparation method and application of magnetically loaded ionic liquid microsphere immobilized cells

A technology of immobilized cells and ionic liquids, which is applied in the field of preparation of immobilized cells with magnetically loaded ionic liquid microspheres, can solve the problems of expensive ionic liquids, high cost of use, and low cell reuse rate, and achieve rapid on-line separation And continuous fermentation, good biocompatibility, good effect of reusability

Active Publication Date: 2018-12-07
JIAXING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, as a biocatalytic medium, ionic liquids are expensive, use a large amount, and are difficult to recycle, resulting in high cost of use, which has always been an urgent problem to be solved in the field of biocatalysis.
[0008] During the biotransformation process of free microbial cells, there are disadvantages such as low cell reuse rate, large amount of bacteria required, easy to be contaminated by inverted tanks, and sensitive to the culture environment.

Method used

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  • Preparation method and application of magnetically loaded ionic liquid microsphere immobilized cells
  • Preparation method and application of magnetically loaded ionic liquid microsphere immobilized cells
  • Preparation method and application of magnetically loaded ionic liquid microsphere immobilized cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] A method for preparing magnetically loaded ionic liquid microspheres to immobilize cells, the specific steps are as follows:

[0065] (1) Preparation of chitosan (CS) coated ferric oxide (Fe) by co-precipitation method 3 o 4 ) nanoparticles Fe 3 o 4 / CS:

[0066] Prepare sodium acetate solution in a beaker, add chitosan, acetic acid, and ultrasonically dissolve to obtain solution I; take another beaker of sodium acetate solution, add Fe 2+ and Fe 3+ FeSO with a molar ratio of 1:2 4 and FeCl 3 , ultrasonically dissolved to obtain solution II; after mixing solution I and solution II, mixed solution III was obtained, and the mixed solution III was transferred to a constant pressure dropping funnel, and then the dropping funnel was placed on a three-necked flask containing NaOH solution , under the protection of nitrogen, slowly drop the mixed solution into the lye, after the drop, react at 55°C for 1 hour, wash with a large amount of distilled water until neutral, fil...

Embodiment 2

[0096] Fe 3 o 4 , Fe 3 o 4 / CS and magnetic load [BMIM]PF 6 Microspheres (microspheres of unfixed bacterial cells, the preparation method is shown in Example 1) electron microscope analysis: adopt scanning electron microscope (SEM) and transmission electron microscope (TEM) to analyze and observe Fe 3 o 4 and Fe 3 o 4 / CS Morphology.

[0097] Depend on figure 2 a It can be seen that Fe 3 o 4 The nanoparticles are uniformly dispersed, small spherical particles in a discrete and disordered state, the particle size is uniform, the average particle size is about 20nm, and the dispersion is good; figure 2 b It can be seen that the prepared Fe 3 o 4 / CS nanoparticles, the dispersion is slightly poor, the particle size is about 30nm, resulting in Fe 3 o 4 The reason for the slightly poor dispersion of / CS nanoparticles may be that the nanoparticles have a large specific surface area, high surface energy, and the magnetostatic interaction between nanoparticles causes a...

Embodiment 3

[0101] Infrared (FT-IR) analysis: using an infrared spectrometer to analyze chitosan, Fe 3 o 4 , Fe 3 o 4 / CS, [BMIM]PF 6 、Magnetic load[BMIM]PF 6 Microsphere (preparation method sees embodiment 1) carries out infrared characterization, and the result is as follows Figure 5 shown.

[0102] Infrared spectrum a, at 3402cm -1 The broad absorption peak is the vibration absorption peak of hydroxyl-OH, 2925cm -1 is-CH 2 - Stretching vibration absorption peak of middle-C-H; 1625cm -1 Yes-NH 2 Bending vibration absorption peak; 1082cm -1 is the C-O-C stretching vibration peak.

[0103] Infrared spectrum c, Fe 3 o 4 The characteristic peak is 585cm -1 ; Infrared spectrum b shows that after using the coprecipitation method to prepare Fe 3 o 4 / CS at 585cm -1 There are also absorption peaks, and compared with the CS infrared spectrum a, the prepared sample has the characteristic absorption peaks of chitosan, as can be seen from the above analysis, Fe 3 o 4 The particl...

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Abstract

The invention discloses a preparation method and application of magnetically loaded ionic liquid microsphere immobilized cells. The preparation method comprises the following steps of: mixing sodium alginate, a Fe3O4 / CS magnetic material and 1-butyl-3-methylimidazolium hexafluorophosphate to obtain a mixed solution; mixing the mixed solution with cell suspension to obtain injectable suspension; and immobilizing the injectable suspension by using CaCl2 as a crosslinking agent to obtain the magnetically loaded ionic liquid microsphere immobilized cells. As the immobilized cells prepared by the method have the magnetism, the separation and continuous fermentation of immobilized cells are facilitated, and ionic liquid loaded on a magnetic material greatly improves the catalytic activity of theimmobilized cells.

Description

technical field [0001] The invention relates to the technical field of biologically immobilized cells, in particular to a preparation method and application of magnetically loaded ionic liquid microspheres immobilized cells. Background technique [0002] Ionic liquids (ILs) refer to organic molten salts composed of anions and cations that are liquid at room temperature. As a new type of green medium, ionic liquids are forced by the strong desire of the international community for green and clean production, environmental protection, and circular economy, as well as the scientific exploration value and huge application potential of ionic liquids themselves. [0003] The reports on the application of ionic liquids in biocatalysis are increasing year by year, showing a good momentum of development. The biotransformation process of ionic liquids as co-solvents combined with many enzymes and substrates has become an important research topic. [0004] Literature (Li Yang, Cao Hon...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/14
CPCC12N11/10C12N11/14
Inventor 曹红黄晓林金宏杰
Owner JIAXING UNIV
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