Specific primer and kit for detecting gene polymorphism of cardiovascular disease drugs by virtue of liquid-phase chip technique

Abstract

The invention discloses a primer group for detecting the gene polymorphism of cardiovascular disease drugs by virtue of a liquid-phase chip technique. The primer group comprises 15 groups of primers,each group of the primers include a pair of specific amplification primers for detecting the polymorphism of a target gene as well as a wide ASPE primer and a mutant ASPE primer respectively aiming atthe target gene. The invention further discloses a kit including the primer group and a use method of the kit. The primer group and the kit can be used for amplifying a target sequence through one-step PCR reaction and simultaneously detecting the polymorphism of relevant target genes of 15 common cardiovascular drugs through the combination of MagPlex microspheres, the detection flux and the cost performance are high, the medication guidance can be provided for patients with cardiovascular diseases through once detection, and laboratory basis can be provided for the clinical rational drug use; and the kit has very high sensitivity, stability and accuracy in the actual use and is well matched with a gene sequencing result, and the detection cost is greatly lowered.

Description

technical field [0001] The invention relates to the fields of biotechnology and medicine, in particular to specific primers for detecting polymorphisms of commonly used drugs for cardiovascular diseases using liquid-phase chip technology. The invention also relates to a kit comprising these specific primers and a method for using the kit. Background technique [0002] Cardiovascular disease is caused by disorders of the heart and blood vessels, including coronary heart disease (heart attack), cerebrovascular disease (stroke), hypertension (high blood pressure), peripheral vascular disease, rheumatic heart disease, congenital heart disease, cardiac failure and cardiomyopathy. Cardiovascular disease is a common disease that seriously threatens the health of human beings, especially the middle-aged and elderly people over 50 years old. At present, cardiovascular disease death accounts for the first cause of death among urban and rural residents, with 45.01% in rural areas and ...

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Problems solved by technology

[0012] In the prior art, PCR-direct sequencing method, PCR-pyrosequencing method, fluorescent quantitative PCR method, PCR-high resolution melting curve method, allele-specific PCR method, PCR-restriction fragment length polymorphism method are often used Methods and solid chip methods are used to detect polymorphisms of one or several cardiovascular drug-related genes. Most of these methods have low flexibility, high cost, low throughput, long time, expensive detection equipment, and are not suitable for clinical promotion. , the cost of reagents for one inspection per person is tens to hundreds of dollars, and the existing detection kits do not target the spectrum of Chinese genetic diseases

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