Method of removing inorganic salt from phenylalanine fermented liquid

A technology of phenylalanine and fermented liquid, applied in the field of separation and purification, can solve problems such as environmental pollution, affecting product quality, and large water consumption, and achieve the effects of simple process control, shortened production cycle, and low loss rate

Inactive Publication Date: 2018-12-14
SHENGSHIYAOLAN SHENZHEN TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the yield of phenylalanine in this method is unstable, the crude product has many impurities and deep color, which affects the quality of subsequent products, and the entire ion exchange process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Step S1: 200L of phenylalanine fermentation broth was ultrafiltered through polyvinyl chloride membrane to obtain phenylalanine ultrafiltrate. The specific process conditions are: the operating pressure of ultrafiltration is 0.3Mpa. The conductivity of the phenylalanine ultrafiltrate is 34329us / cm, the content of phenylalanine is 12.56%, the content of ammonium salt is 8.8g / L, the content of phosphate is 4.2g / L, and the content of calcium salt is 0.2g / L , the magnesium salt content is 2g / L.

[0045] Step S2: adjusting the pH of the phenylalanine ultrafiltrate to 5.0.

[0046] Step S3: Take 20L of phenylalanine ultrafiltrate as a desalination solution and inject it into the fresh water chamber of the first electrodialysis device, and the initial solution of the concentrated water chamber is 20L of 0.05% NaSO 4 Solution, the initial solution of the pole water chamber is 10L 2% NaSO 4 solution. Start the concentrated water pump, fresh water pump and polar wa...

Embodiment 2

[0050] Example 2: Step S1: 200L of phenylalanine fermentation broth was ultrafiltered through a polyvinylidene fluoride membrane to obtain phenylalanine ultrafiltrate. The specific process conditions are: the operating pressure of ultrafiltration is 0.3Mpa. The conductivity of the phenylalanine ultrafiltrate is 34329us / cm, the content of phenylalanine is 12.56%, the content of ammonium salt is 8.8g / L, the content of phosphate is 4.2g / L, and the content of calcium salt is 0.2g / L , the magnesium salt content is 2g / L.

[0051] Step S2: adjusting the pH of the phenylalanine ultrafiltrate to 6.0.

[0052] Step S3: Take 20L of phenylalanine ultrafiltrate as a desalination solution and inject it into the fresh water chamber of the first electrodialysis device, and the initial solution of the concentrated water chamber is 20L of 0.03% NaSO 4 Solution, the initial solution of the pole water chamber is 10L 2% NaSO 4 solution. Start the concentrated water pump, fresh water pump and p...

Embodiment 3

[0056] Example 3: Step S1: 200L of phenylalanine fermentation broth was ultrafiltered through polyvinylidene fluoride membrane to obtain phenylalanine ultrafiltrate. The specific process conditions are: the operating pressure of ultrafiltration is 0.3Mpa. The conductivity of the phenylalanine ultrafiltrate is 34329us / cm, the content of phenylalanine is 12.56%, the content of ammonium salt is 8.8g / L, the content of phosphate is 4.2g / L, and the content of calcium salt is 0.2g / L , the magnesium salt content is 2g / L.

[0057] Step S2: adjusting the pH of the phenylalanine ultrafiltrate to 7.0.

[0058] Step S3: Take 20L of phenylalanine ultrafiltrate as a desalination solution and inject it into the fresh water chamber of the first electrodialysis device, and the initial solution of the concentrated water chamber is 20L of 0.08% NaSO 4 Solution, the initial solution of the pole water chamber is 10L 5% NaSO 4 solution. Start the concentrated water pump, fresh water pump and pol...

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Abstract

The invention relates to a method of removing inorganic salt from phenylalanine fermented liquid. The method includes: performing ultrafiltration to a phenylalanine fermented liquid through an ultrafiltration membrane, regulating pH of a phenylalanine ultrafiltered clear liquid, injecting the phenylalanine ultrafiltered clear liquid into a first electrodialysis device for desalting the liquid; when the electrical conductivity of the phenylalanine ultrafiltered clear liquid is 5000-6000 [mu]s/cm, injecting the clear liquid into a second electrodialysis device, and crystallizing the second batchof the phenylalanine electrodialyzed concentrated liquid; separating the crystal from the mother liquid and drying the crystal to prepare phenylalanine crystal. The method is simple in control and isfree of addition of chemical substances and generation of solid residues during treatment, wherein the generated waste liquid can be recycled for the production of fermentation, so that the method isfree of environmental pollution. The method is suitable for being promoted and applied.

Description

technical field [0001] The invention relates to the technical field of separation and purification, in particular to a method for removing inorganic salts in phenylalanine fermentation broth by electrodialysis. Background technique [0002] Phenylalanine is an α-amino acid, which is one of the essential amino acids for the human body. It is an aromatic amino acid. Most of it is oxidized into tyrosine by phenylalanine hydroxylase in the body and synthesized together with tyrosine. Important neurotransmitters and hormones, involved in the body's glucose metabolism and fat metabolism. In recent years, the research and development of phenylalanine has attracted extensive attention from the pharmaceutical, biochemical, food and other industries. It is an important component of compound amino acid infusion; used in medicine, it is the intermediate of amino acid anticancer drugs such as phentermine and formic acid sarcoma, and is also the raw material for the production of adrenal...

Claims

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Application Information

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IPC IPC(8): B01D61/42C07C229/36C07C227/42
CPCB01D61/422C07C227/42C07C229/36
Inventor 龚淼
Owner SHENGSHIYAOLAN SHENZHEN TECH CO LTD
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