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Germplasm in-vitro preservation method for mint

A technology of in vitro preservation and mint, applied in the fields of biotechnology and agricultural science, can solve the problem of less research on germplasm preservation, and achieve the effects of prolonging the germplasm preservation period, saving land, and easily restoring growth.

Inactive Publication Date: 2018-12-18
LIANYUNGANG XIUJING LANDSCAPING ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, researches on peppermint in my country mainly focus on pharmacological effects, extraction of volatile oil and non-volatile components, reproduction and cultivation, cultivation management, etc., but few studies on its germplasm preservation

Method used

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  • Germplasm in-vitro preservation method for mint

Examples

Experimental program
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Effect test

Embodiment 1

[0037] In 2012-2015, according to the technical route of the present invention, the in vitro preservation test of peppermint germplasm was carried out, and the detailed steps were as follows:

[0038] (1) Pretreatment of mint plants

[0039] In August 2012, select healthy and disease-free 1-year-old mint plants cultivated in the open field, rinse the whole plant with tap water, especially the root soil, and then immerse the plant root in a nutrient solution containing 0.2mg / L TDZ (nutrient The liquid formula is Ca(H 2 PO 4 ) 2 ·H 2 O 0.4g / L, Ca(NO 3 ) 2 0.5g / L, KNO 3 0.9g / L, urea 0.3g / L, MgSO 4 ·7H 2 O0.28g / L FeNa 2 -EDTA 0.12g / L, H 3 BO 3 0.06mg / L, MnSO 4 4H 2 O 0.03mg / L, ZnSO 4 ·7H 2 O0.06mg / L, CuSO 4 ·5H 2 O 0.06mg / L, (NH 4 ) 2 MoO 4 0.06mg / L, spermidine 3.4mg / L), 4 ℃ low temperature pretreatment for 5-8 days;

[0040] (2) Explant disinfection and inoculation

[0041] Dip the pretreated mint plants with a little detergent with a brush, and rinse th...

Embodiment 2

[0049] Use the same germplasm source and technical route as in Example 1 for germplasm preservation, where the germplasm preservation medium in step (4) is replaced by MS+sucrose 40g / L+mannitol 10g / L (the formula comes from Cihuiting "In Vitro Preservation of Peppermint Test-tube Seedlings"), the survival rate of adventitious buds and average plant height growth were counted after germplasm preservation for 3 years.

Embodiment 3

[0051] Use the same germplasm source and technical route as in Example 1 for germplasm preservation, wherein the germplasm preservation medium in step (4) is replaced by MS medium, and the survival rate of adventitious buds and the average strain are counted after 3 years of germplasm preservation High growth volume.

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Abstract

The invention discloses a germplasm in-vitro preservation method for mint, including: (1) pretreatment of mint plants; (2) explant disinfection and inoculation; (3) induced culture of adventitious buds; (4) germplasm preservation and culture; (5) rejuvenation of the germplasm; (6) seedling culture; (7) acclimatization and transplant. The method, in one preservation period, can preserve the germplasm resource of mint for 3-4 years and greatly prolongs the preservation time; the preserved germplasm has stable inheritable characters; once the germplasm is required, activity can be recovered through the rejuvenation to supply seedlings to production in large scale. The method has very high practical value in germplasm in-vitro preservation.

Description

technical field [0001] The invention belongs to the fields of biotechnology and agricultural science and technology, and relates to a method for in vitro preservation of peppermint germplasm, in particular to a method for utilizing tissue culture technology to realize long-term preservation and post-preservation rejuvenation and propagation of peppermint germplasm. Background technique [0002] Mint( Mentha haplocalyx Briq ) is a perennial herbaceous plant of the Lamiaceae Mentha genus. The whole plant has a strong cool fragrance. It is a plant resource with strong adaptability and wide distribution with a special aromatic smell. Mint is widely distributed in the temperate regions of the northern hemisphere, and it is distributed all over China, both domestically and wild. Among them, Jiangsu and Anhui have the largest cultivation output. Peppermint has dual value of medicine and food. Its dry aerial part can be used as medicine. It has the functions of dispelling wind, dis...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 朱慧玲秦楠楠张翠
Owner LIANYUNGANG XIUJING LANDSCAPING ENG CO LTD
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